Stem cells and cloning : advances and applications.
Publisher:
Dove Medical Press
Frequency: Irregular
Country: New Zealand
Language: English
Start Year:2008 -
ISSN:
1178-6957 (Electronic)
1178-6957 (Linking)
1178-6957 (Linking)
Impact Factor
2.9
2022
| NLM ID: | 101535817 |
| (OCoLC): | 335291958 |
| LCCN: | 2011237250 |
Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses. Cell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem cells (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) as nuclear donors in horse cloning, and we compared the in vitro and in vivo embryo development with respect to fibroblasts. Methods: Zona-free nuclear transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF group, n=4527). Embryos produced by artificial insemination...