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Home / Equine Research Bank / Proc Natl Acad Sci U S A / A DNA-binding protein in the serum of certain mammalian spec...
Proceedings of the National Academy of Sciences of the United States of America1972; 69(11); 3327-3330; doi: 10.1073/pnas.69.11.3327

A DNA-binding protein in the serum of certain mammalian species.

Abstract: Various mammalian species contain an anionic serum protein that reacts specifically with native DNA. It is considerably less reactive with single-strand DNA and does not react with monodeoxyribonucleotides, homopolyribonucleotides, or duplexes of homopolyribonucleotides. Synthetic dA.dT was an effective inhibitor of the reaction with native DNA, while Micrococcus luteus DNA and dG.dC were not inhibitory. This protein was encountered in the course of studies on DNA antibodies. Although it reacted with red cells coated with DNA and gave agar precipitation bands, it was clearly distinct from DNA antibodies. It was found in the serum of all animals of a given species, migrated as an alpha-beta globulin, and did not crossreact with gammaglobulins. It reacted with DNA in solution to give precipitation curves that were strongly influenced by changes in ionic strength. The protein was isolated from canine serum by precipitation with DNA and purified to homogeneity, as judged by immunochemical and electrophoretic criteria.A similar protein was found in mink, equine, and other sera, but not in human sera. Previous studies on DNA antibodies in certain experimental animals may have given false positive results due to this protein.
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Publication Date: 1972-11-01 PubMed ID: 4628875PubMed Central: PMC389764DOI: 10.1073/pnas.69.11.3327Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on the discovery and study of an anionic serum protein found in various mammalian species that reacts specifically with native DNA. It was found that this protein does not exist in humans and its presence could impact previous studies on DNA antibodies in certain experimental animals due to potential false positive results.

Primary Findings

  • The study found that many mammalian species contain a specific anionic serum protein that interacts with native DNA. This protein was significantly less reactive with single-strand DNA and did not react with other substances such as monodeoxyribonucleotides, homopolyribonucleotides, or duplexes of homopolyribonucleotides.
  • Synthetic dA.dT contributes to the inhibition of the reaction with native DNA, whilst Micrococcus luteus DNA and dG.dC did not seem to hinder the process.

Distinguishing Features of The Serum Protein

  • This protein was identified during DNA antibody studies. Despite reacting with DNA-coated red blood cells and creating agar precipitation bands, it was expressly distinct from DNA antibodies.
  • Notably, the protein was found in the serum of every animal of a given species examined. It migrated as an alpha-beta globulin and showed no cross-reactivity with gammaglobulins.

Protein Behavior

  • The protein exhibited a strong interaction with DNA in solution to yield precipitation curves, with the results showing considerable modification as the ionic strength was altered.

Isolation and Examination of The Serum Protein

  • Through the use of DNA precipitation, the protein was isolated from canine serum and subsequently purified to homogeneity, as confirmed by immunochemical and electrophoretic criteria.
  • Interestingly, similar proteins were found in other animal serums such as mink and equine,but not in humans. This potentially suggests that studies that have previously been carried out on DNA antibodies using non-human subjects may have given false positive results due to the presence of this protein.

Cite This Article

APA
Thoburn R, Hurvitz AI, Kunkel HG. (1972). A DNA-binding protein in the serum of certain mammalian species. Proc Natl Acad Sci U S A, 69(11), 3327-3330. https://doi.org/10.1073/pnas.69.11.3327

Publication

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
NlmUniqueID: 7505876
Country: United States
Language: English
Volume: 69
Issue: 11
Pages: 3327-3330

Researcher Affiliations

Thoburn, R
    Hurvitz, A I
      Kunkel, H G

        MeSH Terms

        • Animals
        • Blood Proteins / isolation & purification
        • Blood Proteins / metabolism
        • Cats
        • Cattle
        • DNA / metabolism
        • DNA, Bacterial / metabolism
        • DNA, Single-Stranded / metabolism
        • Dogs
        • Electrophoresis, Polyacrylamide Gel
        • Escherichia coli
        • Haplorhini
        • Hemagglutination Inhibition Tests
        • Hemagglutination Tests
        • Horses
        • Humans
        • Immunodiffusion
        • Male
        • Mice
        • Mink
        • Protein Binding
        • Rabbits
        • Salmon
        • Sheep
        • Species Specificity
        • Spermatozoa
        • Thymus Gland

        References

        This article includes 20 references
        1. Tan EM, Schur PH, Carr RI, Kunkel HG. Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus.. J Clin Invest 1966 Nov;45(11):1732-40.
          pubmed: 4959277doi: 10.1172/JCI105479google scholar: lookup
        2. Plescia OJ, Braun W. Nucleic acids as antigens.. Adv Immunol 1967;6:231-52.
          pubmed: 4860247doi: 10.1016/s0065-2776(08)60523-4google scholar: lookup
        3. Lambert PH, Dixon FJ. Pathogenesis of the glomerulonephritis of NZB/W mice.. J Exp Med 1968 Mar 1;127(3):507-22.
          pubmed: 4169964doi: 10.1084/jem.127.3.507google scholar: lookup
        4. Kleinsmith LJ, Allfrey VG. Nuclear phosphoproteins. I. Isolation and characterization of a phosphoprotein fraction from calf thymus nuclei.. Biochim Biophys Acta 1969 Feb 4;175(1):123-35.
          pubmed: 5765997
        5. Steinberg AD, Pincus T, Talal N. DNA-binding assay for detection of anti-DNA antibodies in NZB-NZW F1 mice.. J Immunol 1969 Mar;102(3):788-90.
          pubmed: 5304991
        6. Barnett EV, Williams RC Jr, Kenyon AJ, Henson JE. 'Nuclear' antigens and antinuclear antibodies in mink sera.. Immunology 1969 Feb;16(2):241-53.
          pubmed: 4181511
        7. Englund PT, Kelly RB, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXXI. Binding of deoxyribonucleic acid to deoxyribonucleic acid polymerase.. J Biol Chem 1969 Jun 10;244(11):3045-52.
          pubmed: 4890764
        8. Koffler D, Carr RI, Agnello V, Fiezi T, Kunkel HG. Antibodies to polynucleotides: distribution in human serums.. Science 1969 Dec 26;166(3913):1648-9.
          pubmed: 5360590doi: 10.1126/science.166.3913.1648google scholar: lookup
        9. Alberts BM. Function of gene 32-protein, a new protein essential for the genetic recombination and replication of T4 bacteriophage DNA.. Fed Proc 1970 May-Jun;29(3):1154-63.
          pubmed: 4910266
        10. Teng CT, Teng CS, Allfrey VG. Species-specific interactions between nuclear phosphoproteins and DNA.. Biochem Biophys Res Commun 1970 Nov 9;41(3):690-6.
          pubmed: 5479295doi: 10.1016/0006-291x(70)90068-9google scholar: lookup
        11. Agnello V, Winchester RJ, Kunkel HG. Precipitin reactions of the C1q component of complement with aggregated gamma-globulin and immune complexes in gel diffusion.. Immunology 1970 Dec;19(6):909-19.
          pubmed: 4098878
        12. Thoburn R, Koffler D, Kunkel HG. Distribution of antibodies to native DNA, single-stranded DNA, and double-stranded RNA in mouse serums.. Proc Soc Exp Biol Med 1971 Mar;136(3):711-4.
          pubmed: 5555360doi: 10.3181/00379727-136-35347google scholar: lookup
        13. Hillyer GV. Deoxyribonucleic acid (DNA) and antibodies to DNA in the serum of hamsters and man infected with schistosomes.. Proc Soc Exp Biol Med 1971 Mar;136(3):880-3.
          pubmed: 4995331doi: 10.3181/00379727-136-35386google scholar: lookup
        14. Salas J, Green H. Proteins binding to DNA and their relation to growth in cultured mammalian cells.. Nat New Biol 1971 Feb 10;229(6):165-9.
          pubmed: 5280102doi: 10.1038/newbio229165a0google scholar: lookup
        15. Koffler D, Carr R, Agnello V, Thoburn R, Kunkel HG. Antibodies to polynucleotides in human sera: antigenic specificity and relation to disease.. J Exp Med 1971 Jul 1;134(1):294-312.
          pubmed: 4997587doi: 10.1084/jem.134.1.294google scholar: lookup
        16. LeStourgeon WM, Rusch HP. Nuclear acidic protein changes during differentiation in Physarum polycephalum.. Science 1971 Dec 17;174(4015):1233-6.
          pubmed: 5167331doi: 10.1126/science.174.4015.1233google scholar: lookup
        17. KUNKEL HG, TISELIUS A. Electrophoresis of proteins on filter paper.. J Gen Physiol 1951 Sep;35(1):89-118.
          pubmed: 14873923doi: 10.1085/jgp.35.1.89google scholar: lookup
        18. SELIGMANN M, MILGROM F. [Demonstration by complement fixation the reaction between desoxyribonucleic acid and serum of patients with disseminated lupus erythematosus].. C R Hebd Seances Acad Sci 1957 Oct 21;245(17):1472-5.
          pubmed: 13473214
        19. ROBBINS WC, HOLMAN HR, DEICHER H, KUNKEL HG. Complement fixation with cell nuclei and DNA in lupus erythematosus.. Proc Soc Exp Biol Med 1957 Dec;96(3):575-9.
          pubmed: 13505796doi: 10.3181/00379727-96-23545google scholar: lookup
        20. DAVIS BJ. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.. Ann N Y Acad Sci 1964 Dec 28;121:404-27.
          pubmed: 14240539doi: 10.1111/j.1749-6632.1964.tb14213.xgoogle scholar: lookup

        Citations

        This article has been cited 11 times.
        1. Cheow LF, Han J. Continuous signal enhancement for sensitive aptamer affinity probe electrophoresis assay using electrokinetic concentration.. Anal Chem 2011 Sep 15;83(18):7086-93.
          doi: 10.1021/ac201307dpubmed: 21809885google scholar: lookup
        2. Bohnhorst JO, Hanssen I, Moen T. Immune-mediated fever in the dog. Occurrence of antinuclear antibodies, rheumatoid factor, tumor necrosis factor and interleukin-6 in serum.. Acta Vet Scand 2002;43(3):165-71.
          doi: 10.1186/1751-0147-43-165pubmed: 12564546google scholar: lookup
        3. Ovrebo Bohnhorst J, Hanssen I, Moen T. Antinuclear antibodies (ANA) in Gordon setters with symmetrical lupoid onychodystrophy and black hair follicular dysplasia.. Acta Vet Scand 2001;42(3):323-9.
          doi: 10.1186/1751-0147-42-323pubmed: 11887392google scholar: lookup
        4. Hansson H, Karlsson-Parra A. Canine antinuclear antibodies: comparison of immunofluorescence staining patterns and precipitin reactivity.. Acta Vet Scand 1999;40(3):205-12.
          doi: 10.1186/BF03547018pubmed: 10605137google scholar: lookup
        5. Monestier M, Novick KE, Karam ET, Chabanne L, Monier JC, Rigal D. Autoantibodies to histone, DNA and nucleosome antigens in canine systemic lupus erythematosus.. Clin Exp Immunol 1995 Jan;99(1):37-41.
          doi: 10.1111/j.1365-2249.1995.tb03469.xpubmed: 7529150google scholar: lookup
        6. Bearn AG, Dixon FJ, Benacerraf B. Henry G. Kunkel 1916-1983. An appreciation of the man and his scientific contributions & a bibliography of his research papers.. J Exp Med 1985 May 1;161(5):869-95.
          doi: 10.1084/jem.161.5.869pubmed: 3886830google scholar: lookup
        7. Powell RJ, Jones DR. Can man's best friend provide clues to the aetiology of systemic lupus erythematosus?. Ann Rheum Dis 1992 Jul;51(7):833-4.
          doi: 10.1136/ard.51.7.833pubmed: 1632656google scholar: lookup
        8. Steinman CR, Deesomchok U, Spiera H. Detection of anti-DNA antibody using synthetic antigens. Characterization and clinical significance of binding of poly (deoxyadenylate-deoxythymidylate) by serum.. J Clin Invest 1976 May;57(5):1330-41.
          doi: 10.1172/JCI108401pubmed: 1262474google scholar: lookup
        9. Russell AS, Percy JS, Cho HJ. Poly IC therapy in aleutian disease of mink.. Can J Comp Med 1975 Jul;39(3):240-9.
          pubmed: 1095164
        10. Roberts DB, Andrews PW. Drosophila chromatin: an immunological study.. Nucleic Acids Res 1975 Aug;2(8):1291-303.
          doi: 10.1093/nar/2.8.1291pubmed: 809756google scholar: lookup
        11. Reimer G, Maibach M, Leonhardi G. DNA-binding proteins in the sera of patients with malignant melanoma.. Arch Dermatol Res 1979 May 4;264(3):265-73.
          doi: 10.1007/BF00412653pubmed: 223503google scholar: lookup
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