FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Archiv fur die gesamte Virusforschung1969; 28(1); 26-33; doi: 10.1007/BF01250842

A plaque assay of equine arteritis virus in BHK-21 cells.

Abstract: No abstract available
Get Full Text
Publication Date: 1969-01-01 PubMed ID: 4988871DOI: 10.1007/BF01250842Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article deals with studying the growth of equine arteritis virus (EAV) in different cells: BHK-21, primary rabbit kidney and primary horse testicle cells, and the impact of DEAE-Dextran on EAV’s plaque formation.

Experiments with Different Types of Cells

  • The researchers used three types of cells to conduct their study regarding equine arteritis virus (EAV), including BHK-21, primary rabbit kidney and primary horse testicle cells.
  • The Bucyrus strain of EAV was utilized for these experiments, which was grown in the mentioned cells.
  • The team observed distinct plaques formation in BHK-21 cells from the viral yields (the amount of virus) secured from the different types of cells.

Linear Relationship: Virus Concentration and Plaque Count

  • The researchers discovered a linear relationship between the virus concentration and the plaque count.
  • This implies that as the concentration of the virus increased, the number of plaques also increased proportionally, suggesting a directly proportional correlation.

Role of DEAE-Dextran

  • DEAE-Dextran is a type of dextran, a complex branched polysaccharide (type of carbohydrate), remedied with diethylaminoethyl groups, and used for various laboratory purposes.
  • The research team added 50 μg/ml DEAE-Dextran to the diluent (a liquid to make a solution less concentrated) of EAV, which resulted in the formation of the maximum number of plaques.
  • Thus, they concluded that the presence of DEAE-Dextran in the diluent enhances the plaque formation capacity of the EAV strain.

Infection Sensitivity Comparison

  • The team also compared the end-point titers of infectivity among the three types of cells.
  • End-point titers refer to the highest dilutions of a substance at which a specific reaction or effect can still be measured.
  • Despite the earlier observation of distinct plaques in BHK-21 cells, the study concluded that BHK-21 cells were as sensitive to EAV infection as rabbit kidney or horse testicle cells, indicating no significant difference in terms of their vulnerability to EAV.

Cite This Article

APA
Hyllseth B. (1969). A plaque assay of equine arteritis virus in BHK-21 cells. Arch Gesamte Virusforsch, 28(1), 26-33. https://doi.org/10.1007/BF01250842

Publication

Archiv fur die gesamte Virusforschung
ISSN: 0003-9012
NlmUniqueID: 7506868
Country: Austria
Language: English
Volume: 28
Issue: 1
Pages: 26-33

Researcher Affiliations

Hyllseth, B

    MeSH Terms

    • Animals
    • Cell Line
    • Cricetinae
    • Culture Media
    • Culture Techniques
    • Cytopathogenic Effect, Viral
    • Dextrans / pharmacology
    • Horses
    • Kidney
    • Male
    • Rabbits
    • Testis
    • Virus Cultivation
    • Viruses, Unclassified / drug effects
    • Viruses, Unclassified / growth & development
    • Viruses, Unclassified / pathogenicity

    References

    This article includes 16 references
    1. Nature. 1964 Sep 26;203:1355-7
      pubmed: 14207308
    2. Cornell Vet. 1957 Jan;47(1):52-68
      pubmed: 13397179
    3. Proc Soc Exp Biol Med. 1967 Aug-Sep;125(4):1092-8
      pubmed: 4167132
    4. Virology. 1961 Jul;14:316-28
      pubmed: 13766052
    5. Cornell Vet. 1968 Oct;48(4):497-524
      pubmed: 4971878
    6. J Virol. 1968 Jun;2(6):643-4
      pubmed: 4301509
    7. Cornell Vet. 1962 Apr;52:200-5
      pubmed: 14007366
    8. J Virol. 1967 Feb;1(1):145-51
      pubmed: 4990035
    9. Science. 1968 Jan 26;159(3813):390-6
      pubmed: 4169262
    10. Cornell Vet. 1957 Jan;47(1):3-41
      pubmed: 13397177
    11. Pathol Microbiol (Basel). 1965;28(6):939-49
      pubmed: 4956350
    12. J Virol. 1968 Sep;2(9):937-43
      pubmed: 4302190
    13. J Exp Med. 1954 Feb;99(2):167-82
      pubmed: 13130792
    14. Arch Gesamte Virusforsch. 1966;19(2):123-9
      pubmed: 4293706
    15. Virology. 1968 Sep;36(1):155-7
      pubmed: 4299388
    16. Virology. 1961 Jun;14:234-9
      pubmed: 13756521

    Citations

    This article has been cited 10 times.
    1. Balasuriya UB, Zhang J, Go YY, MacLachlan NJ. Experiences with infectious cDNA clones of equine arteritis virus: lessons learned and insights gained. Virology 2014 Aug;462-463:388-403.
      doi: 10.1016/j.virol.2014.04.029pubmed: 24913633google scholar: lookup
    2. Balasuriya UB, Go YY, MacLachlan NJ. Equine arteritis virus. Vet Microbiol 2013 Nov 29;167(1-2):93-122.
      doi: 10.1016/j.vetmic.2013.06.015pubmed: 23891306google scholar: lookup
    3. Veit M, Kabatek A, Tielesch C, Hermann A. Characterization of equine arteritis virus particles and demonstration of their hemolytic activity. Arch Virol 2008;153(2):351-6.
      doi: 10.1007/s00705-007-1094-ypubmed: 18219439google scholar: lookup
    4. van Berlo MF, Horzinek MC, van der Zeijst BA. Equine arteritis virus-infected cells contain six polyadenylated virus-specific RNAs. Virology 1982 Apr 30;118(2):345-52.
      doi: 10.1016/0042-6822(82)90354-3pubmed: 6283728google scholar: lookup
    5. Hyllseth B, Pettersson U. Neutralization of equine arteritis virus: enhancing effect of guinea pig serum. Arch Gesamte Virusforsch 1970;32(4):337-47.
      doi: 10.1007/BF01250061pubmed: 4993579google scholar: lookup
    6. Hyllseth B. Buoyant density studies on equine arteritis virus. Arch Gesamte Virusforsch 1970;30(2):97-104.
      doi: 10.1007/BF01250176pubmed: 4989678google scholar: lookup
    7. Hyllseth B. Structural proteins of equine arteritis virus. Arch Gesamte Virusforsch 1973;40(3):177-88.
      doi: 10.1007/BF01242536pubmed: 4633581google scholar: lookup
    8. Diderholm H, Hyllseth B, Dinter Z. Inhibition of hog cholera virus by acriflavine. A property shared with bovine virus diarrhoea virus. Brief report. Arch Gesamte Virusforsch 1973;42(3):300-2.
      doi: 10.1007/BF01265656pubmed: 4356773google scholar: lookup
    9. Magnusson P, Hyllseth B, Marusyk H. Morphological studies on equine arteritis virus. Arch Gesamte Virusforsch 1970;30(2):105-12.
      doi: 10.1007/BF01250177pubmed: 4195609google scholar: lookup
    10. Plagemann PG, Moennig V. Lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Adv Virus Res 1992;41:99-192.
      doi: 10.1016/s0065-3527(08)60036-6pubmed: 1315480google scholar: lookup
    Subscribe to our newsletter
    Join 99,727 horse owners like you who receive our email updates on horse health and nutrition.