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Reproduction in domestic animals = Zuchthygiene2024; 59(7); e14663; doi: 10.1111/rda.14663

A prospective study of the proteome of equine pre-implantation embryo.

Abstract: The present study was conducted to investigate the global proteome of 8-day-old equine blastocysts. Follicular dynamics of eight adult mares were monitored by ultrasonography and inseminated 24 h after the detection of a preovulatory follicle. Four expanded blastocysts were recovered, pooled, and subjected to protein extraction and mass spectrometry. Protein identification was conducted based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, and PepExplorer). Enrichment analysis was performed using g:Profiler, Panther, and String platforms. After the elimination of identification redundancies among search tools (at three levels, based on identifiers, peptides, and cross-database mapping), 1977 proteins were reliably identified in the samples of equine embryos. Proteomic analysis unveiled robust metabolic activity in the 8-day equine embryo, highlighted by an abundance of proteins engaged in key metabolic pathways like the TCA cycle, ATP biosynthesis, and glycolysis. The prevalence of chaperones among highly abundant proteins suggests that regulation of protein folding, and degradation is a key process during embryo development. These findings pave the way for developing new strategies to improve equine embryo media and optimize in vitro fertilization techniques.
© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.
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Publication Date: 2024-07-11 PubMed ID: 38990011DOI: 10.1111/rda.14663Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on exploring the protein composition of 8-day-old equine embryos, with an aim to understand the metabolic activities during early development. The insights from this examination can pave the way for more effective strategies to improve in vitro fertilization techniques for equines.

Study Procedure

  • The researchers monitored the follicular dynamics – the growth and development of ovarian follicles – of eight adult mares using ultrasonography.
  • After detecting a preovulatory follicle – a mature egg-releasing follicle – the mares were inseminated within 24 hours.
  • Expanded blastocysts – an advanced stage embryo – were then recovered, pooled together, and subjected to protein extraction and mass spectrometry. Mass spectrometry is a technique for measuring the mass and concentration of atoms and molecules.
  • The proteins in these samples were then identified using four different database searches.

Data Analysis

  • The team performed enrichment analysis – a process to identify significantly overused or underused data categories – using three different platforms (g:Profiler, Panther, and String).
  • The researchers then eliminated redundant identifications at three levels, based on identifiers, peptides, and cross-database mapping.
  • A total of 1977 proteins were efficiently identified in the samples of equine embryos.

Findings

  • The proteomic analysis, a study of the protein content, revealed strong metabolic activity in the 8-day old equine embryo.
  • The embryos featured a variety of proteins involved in key metabolic pathways like the TCA (Tricarboxylic Acid) cycle, ATP (Adenosine Triphosphate) biosynthesis and glycolysis.
  • The abundance of chaperones among the proteins indicate that processes like protein folding and degradation are vital during embryo development.

Implications

  • The discoveries underline the importance of recognizing and understanding the protein composition of equine embryos, which can contribute to the development of new strategies to optimize in vitro fertilization techniques, as well as improve the composition of the media in which these embryos are cultured in the lab.

Cite This Article

APA
Teles Filho ACA, Sanchez DJD, Viana AGA, Sheheryar S, Guerreiro DD, Bustamante-Filho IC, Martins AMA, Sousa MV, Ricart CAO, Fontes W, Moura AA. (2024). A prospective study of the proteome of equine pre-implantation embryo. Reprod Domest Anim, 59(7), e14663. https://doi.org/10.1111/rda.14663

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 59
Issue: 7
Pages: e14663

Researcher Affiliations

Teles Filho, Antônio Carlos de A
  • Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
Sanchez, Deisy J D
  • Laboratório de Biotecnologia, Universidad Cooperativa de Colombia, Bucaramanga, Colombia.
Viana, Arabela G A
  • Department of Biology, Federal University of Viçosa, Viçosa, Brazil.
Sheheryar, Sheheryar
  • Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
Guerreiro, Denise D
  • Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
Bustamante-Filho, Ivan C
  • Laboratório de Biotecnologia, Universidade Do Vale Do Taquari, Lajeado, Brazil.
Martins, Aline M A
  • Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
Sousa, Marcelo V
  • Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
Ricart, Carlos A O
  • Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
Fontes, Wagner
  • Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
Moura, Arlindo A
  • Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.

MeSH Terms

  • Animals
  • Horses / embryology
  • Proteome
  • Female
  • Blastocyst / metabolism
  • Embryonic Development
  • Prospective Studies
  • Proteomics
  • Fertilization in Vitro / veterinary

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