An unusual pattern of invertase activity development in the thermophilic fungus Thermomyces lanuginosus.
Abstract: In the thermophilic fungus Thermomyces lanuginosus, invertase displays an unusual pattern of development: the induced activity begins to diminish even before any substantial quantity of sucrose has been utilized or an appreciable amount of biomass has been produced. Despite this pattern of invertase activity, neither the growth rate nor the final mycelial yield is affected adversely. T. lanuginosus invertase is a thiol protein and the enzyme is active when specific sulfhydryl group(s) is in the reduced state. Measurements of reduced coenzyme and glutathione pools in sucrose-growth mycelia excluded oxidative stress as the primary reason for the observed decline in invertase activity. Rather, this unusual pattern of invertase is considered to be due to its localization in the hyphal tips. At the early stage of growth, the number of hyphal tips per unit mass of mycelium is maximum, whereas at later times their numbers do not increase in proportion to the biomass. As a result invertase activity shows an apparent inverse relationship with biomass. The enzyme activity disappears when the inducing carbon source is consumed and growth is completed.
Publication Date: 1999-09-04 PubMed ID: 10475745DOI: 10.1111/j.1574-6968.1999.tb13711.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper investigates the unusual pattern of development of invertase activity in the thermophilic fungus Thermomyces lanuginosus. The pattern sees the initiated activity diminish before much sucrose is used or substantial biomass is produced, which doesn’t affect the growth or final yield of the mycelium.
About Thermomyces lanuginosus
- The research focuses on Thermomyces lanuginosus, a type of thermophilic fungus. Thermophilic organisms are those that thrive at relatively high temperatures, between 41 and 122 degrees Celsius.
Invertase Development Pattern
- The development of invertase activity in this fungus shows an aberrant pattern. Unlike what might be expected, the activity begins to minimize even before significant sucrose usage or considerable biomass generation has occurred.
- Interestingly, this peculiar pattern of activity does not negatively affect the growth rate or the ultimate mycelial yield of the fungus.
T. lanuginosus Invertase
- The invertase in T. lanuginosus is a thiol protein. It is active when certain sulfhydryl group(s) are in the reduced state.
- The researchers ruled out oxidative stress as the main reason behind the observed decline in invertase activity by measuring the reduced coenzyme and glutathione pools in sucrose-growth mycelia.
Localization of Invertase and its Effects
- Instead, the unique pattern of invertase activity is considered to be due to its localization in the hyphal tips.
- At the early growth stage, the number of hyphal tips per unit mass of mycelium is at its peak, while at later times, their number does not proportionally increase with the biomass. This results in an apparent inverse relationship between invertase activity and biomass.
- The enzyme activity disappears when the inducing carbon source (sucrose) is consumed and growth is completed.
Cite This Article
APA
Chaudhuri A, Bharadwaj G, Maheshwari R.
(1999).
An unusual pattern of invertase activity development in the thermophilic fungus Thermomyces lanuginosus.
FEMS Microbiol Lett, 177(1), 39-45.
https://doi.org/10.1111/j.1574-6968.1999.tb13711.x Publication
Researcher Affiliations
- Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India.
MeSH Terms
- Animals
- Biomass
- Feces / microbiology
- Glutathione / metabolism
- Glutathione Disulfide / metabolism
- Glycoside Hydrolases / metabolism
- Horses
- Mitosporic Fungi / enzymology
- Mitosporic Fungi / growth & development
- Mitosporic Fungi / isolation & purification
- NADP / metabolism
- Sulfhydryl Compounds / metabolism
- Trehalase / metabolism
- beta-Fructofuranosidase
Citations
This article has been cited 7 times.- Galvão DFA, Pessoni RAB, Elsztein C, Moreira KA, Morais MA, de Cássia Leone Figueiredo-Ribeiro R, Gaspar M, Morais MMC, Fialho MB, Braga MR. A comparative study between Fusarium solani and Neocosmospora vasinfecta revealed differential profile of fructooligosaccharide production. Folia Microbiol (Praha) 2022 Dec;67(6):873-889.
- Pessoni RA, Tersarotto CC, Mateus CA, Zerlin JK, Simões K, de Cássia L Figueiredo-Ribeiro R, Braga MR. Fructose affecting morphology and inducing β-fructofuranosidases in Penicillium janczewskii. Springerplus 2015;4:487.
- Liao X, Fang W, Lin L, Lu HL, St Leger RJ. Metarhizium robertsii produces an extracellular invertase (MrINV) that plays a pivotal role in rhizospheric interactions and root colonization. PLoS One 2013;8(10):e78118.
- Alegre AC, de Lourdes Teixeira de Moraes Polizeli M, Terenzi HF, Jorge JA, Guimarães LH. Production of thermostable invertases by Aspergillus caespitosus under submerged or solid state fermentation using agroindustrial residues as carbon source. Braz J Microbiol 2009 Jul;40(3):612-22.
- Giraldo MA, da Silva TM, Salvato F, Terenzi HF, Jorge JA, Guimarães LH. Thermostable invertases from Paecylomyces variotii produced under submerged and solid-state fermentation using agroindustrial residues. World J Microbiol Biotechnol 2012 Feb;28(2):463-72.
- Vargas WA, Mandawe JC, Kenerley CM. Plant-derived sucrose is a key element in the symbiotic association between Trichoderma virens and maize plants. Plant Physiol 2009 Oct;151(2):792-808.
- Maheshwari R, Bharadwaj G, Bhat MK. Thermophilic fungi: their physiology and enzymes. Microbiol Mol Biol Rev 2000 Sep;64(3):461-88.
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