Antigenic and structural conservation of herpesvirus DNA-binding proteins.
Abstract: Previously, we have shown a common antigen of several herpesviruses (pseudorabies virus, equine abortion virus and bovine mammillitis virus) to be antigenically related to the major DNA-binding proteins of herpes simplex virus types 1 and 2. In this study we have purified the cross-reacting polypeptide from cells infected with pseudorabies virus, equine abortion virus and bovine mammillitis virus and shown the cross-reacting protein to be a major DNA-binding protein for each virus. Tryptic peptide analysis of the cross-reacting DNA-binding proteins of all five viruses has shown structural similarities. The proteins thus were shown to share common antigenic sites, to have similar biological properties and to have a highly conserved amino acid sequence. This unexpected similarity between proteins from diverse herpes viruses suggests an essential and fundamental role of the major DNA-binding protein in herpes virus replication.
Publication Date: 1981-10-01 PubMed ID: 6171613DOI: 10.1099/0022-1317-56-2-409Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article discusses the discovery of shared structural and antigenic features among major DNA-binding proteins of different types of herpesviruses, suggesting their critical role in viral replication.
Objective of the Research
- The main goal of this study was to explore the structural and antigenic properties of the major DNA-binding proteins in several herpesviruses including pseudorabies virus, equine abortion virus, bovine mammillitis virus, and herpes simplex virus types 1 and 2.
Procedure and Findings
- The researchers first purified a common antigen, previously discovered to be a crucial DNA-binding protein for each of these viruses.
- Through tryptic peptide analysis of the cross-reacting DNA-binding proteins from all five virus types, they showed that these proteins share similar biological properties, common antigenic sites, and a highly conserved amino acid sequence.
- The surprising similarity among the proteins from these diverse herpesviruses suggests an essential and fundamental role of the major DNA-binding protein in the process of viral replication.
Significance of the Research
- The discovery of the shared features among the DNA-binding proteins of different herpesviruses increases our understanding of the virus biology and replication mechanism.
- This depth of understanding could potentially lead to the development of more targeted treatment and prevention strategies against various types of herpesvirus infections.
- The identification of these common aspects also creates opportunities for comparative studies among various herpesviruses, which could generate new insights into their structure and function.
Cite This Article
APA
Littler E, Yeo J, Killington RA, Purifoy DJ, Powell KL.
(1981).
Antigenic and structural conservation of herpesvirus DNA-binding proteins.
J Gen Virol, 56(Pt 2), 409-419.
https://doi.org/10.1099/0022-1317-56-2-409 Publication
Researcher Affiliations
MeSH Terms
- Amino Acid Sequence
- Carrier Proteins / analysis
- Carrier Proteins / immunology
- Cross Reactions
- DNA-Binding Proteins
- Epitopes
- Herpesviridae / analysis
- Herpesviridae / immunology
- Herpesvirus 1, Equid / analysis
- Herpesvirus 1, Suid / analysis
- Peptides / analysis
- Simplexvirus / analysis
- Viral Proteins / analysis
- Viral Proteins / immunology
Grant Funding
- AI 15034 / NIAID NIH HHS
Citations
This article has been cited 7 times.- Bell CW, Whalley JM. Herpesvirus ICP18.5 and DNA-binding protein genes are conserved in equine herpesvirus-1.. Virus Genes 1993 Sep;7(3):219-28.
- Weller SK, Lee KJ, Sabourin DJ, Schaffer PA. Genetic analysis of temperature-sensitive mutants which define the gene for the major herpes simplex virus type 1 DNA-binding protein.. J Virol 1983 Jan;45(1):354-66.
- Ruyechan WT, Weir AC. Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein.. J Virol 1984 Dec;52(3):727-33.
- Balachandran N, Oba DE, Hutt-Fletcher LM. Antigenic cross-reactions among herpes simplex virus types 1 and 2, Epstein-Barr virus, and cytomegalovirus.. J Virol 1987 Apr;61(4):1125-35.
- Roberts CR, Weir AC, Hay J, Straus SE, Ruyechan WT. DNA-binding proteins present in varicella-zoster virus-infected cells.. J Virol 1985 Jul;55(1):45-53.
- Kinchington PR, Inchauspe G, Subak-Sharpe JH, Robey F, Hay J, Ruyechan WT. Identification and characterization of a varicella-zoster virus DNA-binding protein by using antisera directed against a predicted synthetic oligopeptide.. J Virol 1988 Mar;62(3):802-9.
- Borchers K, Ozel M, Pauli G, Gelderblom HR, Ludwig H. Conserved epitopes of simian herpesvirus SA 8 and bovine herpesvirus type 2.. Arch Virol 1990;111(1-2):1-14.
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