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Home / Equine Research Bank / Front Microbiol / Antimicrobial resistance and genetic diversity of Klebsiella...
Frontiers in microbiology2024; 14; 1334555; doi: 10.3389/fmicb.2023.1334555

Antimicrobial resistance and genetic diversity of Klebsiella pneumoniae strains from different clinical sources in horses.

Abstract: Klebsiella pneumoniae is a major cause of infections and reproductive disorders among horses, ranked in recent French studies as the sixth most frequently isolated bacterial pathogen in equine clinical samples. The proportion of multidrug-resistant (MDR) K. pneumoniae is therefore significant in a context where MDR K. pneumoniae strains are considered a major global concern by the World Health Organization. Unassigned: In this study, we used a genomic approach to characterize a population of 119 equine K. pneumoniae strains collected by two laboratories specialized in animal health in Normandy (France). We describe the main antibiotic resistance profiles and acquired resistance genes, and specify the proportion of virulence-encoding genes carried by these strains. The originality of our panel of strains lies in the broad collection period covered, ranging from 1996 to 2020, and the variety of sample sources: necropsies, suspected bacterial infections (e.g., genital, wound, allantochorion, and umbilical artery samples), and contagious equine metritis analyses. Unassigned: Our results reveal a remarkable level of genomic diversity among the strains studied and we report the presence of 39% MDR and 9% hypervirulent strains (including 5% that are both MDR and hypervirulent). Unassigned: These findings clearly emphasize the importance of improving the surveillance of K. pneumoniae in routine equine diagnostic tests to detect high-risk MDR-hypervirulent Klebsiella pneumoniae strains. The circulation of these worrisome strains reveals that they are not being detected by the simple K1, K2, and K5 serotype approach currently implemented in the French horse-breeding sector.
Copyright © 2024 Gravey, Sévin, Castagnet, Foucher, Maillard, Tapprest, Léon, Langlois, Le Hello and Petry.
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Publication Date: 2024-01-11 PubMed ID: 38274763PubMed Central: PMC10808340DOI: 10.3389/fmicb.2023.1334555Google Scholar: Lookup
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Summary

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The researchers studied the genetic diversity and antimicrobial resistance of the Klebsiella pneumoniae bacteria in horses, revealing a high level of genomic diversity and a significant number of multidrug-resistant strains.

Methodology

  • The diverse population of 119 equine Klebsiella pneumoniae strains used in the study was collected by two specialized animal health laboratories in Normandy, France. The strains were gathered over a lengthy period, from 1996 to 2020.
  • This wide spectrum of samples came from various sources including necropsies, suspected bacterial infections (like genital, wound, allantochorion, and umbilical artery samples), and contagious equine metritis analyses.

Findings

  • The study established a high degree of genomic diversity among the Klebsiella pneumoniae strains.
  • The team found that there were considerable levels of multidrug-resistant strains, representing 39% of the total strains.
  • The study also revealed the presence of 9% hypervirulent strains, with 5% of the overall strains showing both multidrug resistance and hypervirulence.

Implications

  • The researchers emphasized the need to improve the surveillance and detection of Klebsiella pneumoniae in routine equine diagnostic tests. Increasing these measures could help detect high-risk, multidrug-resistant, and hypervirulent strains of the bacteria.
  • Interestingly, these problematic strains of Klebsiella pneumoniae are not currently being identified through the traditional K1, K2, and K5 serotype approach used in the French horse-breeding sector.

Conclusion

  • The study reveals an urgent need for developing alternative strategies for detecting problematic Klebsiella pneumoniae strains to curb the rising incident of multidrug-resistant infections in horses.

Cite This Article

APA
Gravey F, Sévin C, Castagnet S, Foucher N, Maillard K, Tapprest J, Léon A, Langlois B, Le Hello S, Petry S. (2024). Antimicrobial resistance and genetic diversity of Klebsiella pneumoniae strains from different clinical sources in horses. Front Microbiol, 14, 1334555. https://doi.org/10.3389/fmicb.2023.1334555

Publication

Frontiers in microbiology
ISSN: 1664-302X
NlmUniqueID: 101548977
Country: Switzerland
Language: English
Volume: 14
Pages: 1334555

Researcher Affiliations

Gravey, Francois
  • Department of Infectious Agents, Bacteriology, Université de Caen Normandie, Université de Rouen Normandie, INSERM, DYNAMICURE UMR1311, CHU Caen, Caen, France.
  • Department of Infectious Agents, Bacteriology, CHU Caen, Caen, France.
Sévin, Corinne
  • ANSES, Normandy Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France.
Castagnet, Sophie
  • Department of Infectious Agents, Bacteriology, Université de Caen Normandie, Université de Rouen Normandie, INSERM, DYNAMICURE UMR1311, CHU Caen, Caen, France.
  • Research Department, LABÉO, Caen, France.
Foucher, Nathalie
  • ANSES, Normandy Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France.
Maillard, Karine
  • Research Department, LABÉO, Caen, France.
Tapprest, Jackie
  • ANSES, Normandy Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France.
Léon, Albertine
  • Department of Infectious Agents, Bacteriology, Université de Caen Normandie, Université de Rouen Normandie, INSERM, DYNAMICURE UMR1311, CHU Caen, Caen, France.
  • Research Department, LABÉO, Caen, France.
Langlois, Bénédicte
  • Department of Infectious Agents, Bacteriology, Université de Caen Normandie, Université de Rouen Normandie, INSERM, DYNAMICURE UMR1311, CHU Caen, Caen, France.
Le Hello, Simon
  • Department of Infectious Agents, Bacteriology, Université de Caen Normandie, Université de Rouen Normandie, INSERM, DYNAMICURE UMR1311, CHU Caen, Caen, France.
  • Department of Infectious Agents, Bacteriology, CHU Caen, Caen, France.
Petry, Sandrine
  • ANSES, Normandy Laboratory for Animal Health, Physiopathology and Epidemiology of Equine Diseases Unit, Goustranville, France.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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