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Antioxidative enzymes and expression of rbcL gene as tools to monitor heavy metal-related stress in plants.
Abstract: The aim of the study was to evaluate sensitivity and potential applications of selected biomarkers in phytoremediation under complex heavy metal contamination in Sinapis alba L., Robinia pseudoacacia L. and Lupinus luteus L as a potential tools in effective phytoremediation management. The toxicity assessment was conducted using selected measurement endpoints, both classical and advanced, i.e., germination index, roots length, guaiacol peroxidase activity (GPX), chlorophyll and protein content, the amount of total phenolic compounds (TPC) and level of expression of one of the ribulose-bisphosphate carboxylase genes (rbcL). Moreover, the influence of organic additives: cattle, horse manure, and vermicompost on lowering plant abiotic stress caused by complex heavy metal contamination was studied to assess the possible applications of selected stress markers in large scale phytoremediation planning. The results demonstrated the beneficial effects of selected soil additives on plant development. The 5% difference in the quantity of applied amendment caused statistically significant differences in GPX, TPC, chlorophyll content and expression level of rbcL. Among all endpoints, GPX activity, chlorophyll, and phenolic compounds content, as well as the expression of rbcL, turned out to be the most reliable assays for determination of the type and dosage of selected soil amendments (fertilizers) in the assisted phytoremediation process. Selected markers can be used to achieve the desired level of plant abiotic stress and consequently photosynthesis efficiency and CO sequestration. The results showed, that presented assays can be used in different taxonomical groups such as Fabaceae for planning effective phytoremediation process.
Copyright © 2018 Elsevier Ltd. All rights reserved.
Publication Date: 2018-04-14 PubMed ID: 29665488DOI: 10.1016/j.jenvman.2018.04.052Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This research investigates the use of enzymatic and gene expression markers in assessing the impact of heavy metal contamination on several plant species, and evaluating the possible role of organic soil amendments in alleviating this. It underscores the applicability of these markers in guiding phytoremediation efforts across different plant families, such as the Fabaceae.
Research Purpose and Methodology
- The study aimed to assess the sensitivity and applicability of various biomarkers for monitoring heavy metal contamination in Sinapis alba L., Robinia pseudoacacia L., and Lupinus luteus L. These plant species were identified as potential tools for managing efficient phytoremediation activities.
- The toxicity assessment used a variety of traditional and advanced endpoints, such as the germination index, root length, guaiacol peroxidase (GPX) activity, content of chlorophyll and protein, total phenolic compounds (TPC), and the expression level of the ribulose-bisphosphate carboxylase gene (rbcL).
- The study also explored the capacity of organic additives (cattle and horse manure, vermicompost) to reduce plant stress caused by the presence of heavy metals in the environment.
Key Findings
- The study yielded results that demonstrated the beneficial influence of selected soil amendments on plant growth. A 5% difference in the quantity of the applied amendment led to notable differences in the levels of GPX, TPC, chlorophyll and the expression level of the rbcL gene.
- The researchers concluded that GPX activity, chlorophyll, and phenolic compound content, as well as rbcL expression, were reliable indicators for determining the kind and amount of chosen soil amendments (fertilizers) in assisted phytoremediation processes.
- The tested biomarkers could be utilized to achieve the desired level of plant stress and as a result, influence photosynthesis efficiency and carbon dioxide sequestration. It was further suggested that these assays were applicable across different taxonomic groups, including the Fabaceae family, for planning effective phytoremediation strategies.
Cite This Article
APA
Jaskulak M, Rorat A, Grobelak A, Kacprzak M.
(2018).
Antioxidative enzymes and expression of rbcL gene as tools to monitor heavy metal-related stress in plants.
J Environ Manage, 218, 71-78.
https://doi.org/10.1016/j.jenvman.2018.04.052 Publication
Researcher Affiliations
- Institute of Environmental Engineering, Faculty of Infrastructure and Environment, Czestochowa University of Technology, Czestochowa, Poland.
- Université Lille Nord de France, LGCgE-Lille 1, Ecologie Numérique et Ecotoxicologie, F-59650 Villeneuve d'Ascq, France.
- Institute of Environmental Engineering, Faculty of Infrastructure and Environment, Czestochowa University of Technology, Czestochowa, Poland. Electronic address: agrobelak@is.pcz.czest.pl.
- Institute of Environmental Engineering, Faculty of Infrastructure and Environment, Czestochowa University of Technology, Czestochowa, Poland.
MeSH Terms
- Animals
- Antioxidants
- Biodegradation, Environmental
- Cattle
- Horses
- Metals, Heavy
- Plants / genetics
- Ribulose-Bisphosphate Carboxylase / analysis
- Soil
- Soil Pollutants
Citations
This article has been cited 11 times.- Farooq M, Khan MA, Zhao DD, Asif S, Kim EG, Jang YH, Park JR, Lee IJ, Kim KM. Extrinsic role of gibberellin mitigating salinity effect in different rice genotypes. Front Plant Sci 2022;13:1041181.
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- Zhang L, Zou D, Zeng N, Li L, Xiao Z. Slaked lime improves growth, antioxidant capacity and reduces Cd accumulation of peanut (Arachis hypogaea L.) under Cd stress. Sci Rep 2022 Mar 14;12(1):4388.
- Alsherif EA, Al-Shaikh TM, Almaghrabi O, AbdElgawad H. High Redox Status as the Basis for Heavy Metal Tolerance of Sesuvium portulacastrum L. Inhabiting Contaminated Soil in Jeddah, Saudi Arabia. Antioxidants (Basel) 2021 Dec 22;11(1).
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- Gecheva G, Mollov I, Yahubyan G, Gozmanova M, Apostolova E, Vasileva T, Nikolova M, Dimitrova-Dyulgerova I, Radoukova T. Can Biomarkers Respond Upon Freshwater Pollution?-A Moss-Bag Approach. Biology (Basel) 2020 Dec 22;10(1).
- Daundasekara KC, Garza ER, Pepper AE. Plastomic studies inform the mechanisms of edaphic adaptation in North American species in the tribe Thelypodieae (Brassicaceae). Am J Bot 2025 Jul;112(7):e70071.
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