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Brazilian journal of veterinary medicine2024; 46; e005823; doi: 10.29374/2527-2179.bjvm005823

Assessing muscle function and oxidative profile in criollo horses participating in long loop rodeo before and after exercise.

Abstract: Criollo breed is quite popular in Brazil, mainly in the Southern Region, and the long loop rodeo is one of the main sports modality adapted to this breed. The purpose of this study was to assess changes in the muscle and oxidative functions caused by exercises practiced in this sports modality. Data such as exercising frequency, covered distance, mean duration time and number of runs, and blood samples at the following times: before the tournament, on the final day of the event, and 18-24 h after the end of the tournament were collected. Were assessed lactate and the enzymes aspartate aminotransferase and creatine kinase (for the evaluation of the muscle function) and species reactive to oxygen, species reactive to thiobarbituric acid, catalase, superoxide dismutase and uric acid (for oxidative stress). Data were assessed through variance analysis associated with Tukey test (P ≤ 0.05), as well as through Spearman's correlation (P ≤ 0.05). Significant creatine kinase and lactate increase in T1 associated with the maintenance of oxidative indicator levels in T1 and T18 reflected the significant muscle effort and the slight mobilization of oxidative parameters, which were compensated by the antioxidant defenses, since the assessed animals recovered after T18. There was no damage at cell level and the horses showed good muscle and antioxidant condition for exercising. A raça Crioula é bastante difundida no Brasil, principalmente na região Sul, e o rodeio de laço comprido é uma das principais modalidades esportivas desta raça. O objetivo deste estudo foi avaliar as alterações da função muscular e oxidativas provocadas pelo exercício praticado neste tipo de prova. Para isso, foram coletados dados como frequência de treinamento, distância percorrida, tempo médio de duração e número de corridas e amostras de sangue nos tempos: 24 horas anteriores ao início da prova, após a última corrida do último dia de prova e 18 a 24 horas após o final da prova, em provas com duração de dois dias. Foram avaliadas as enzimas aspartato aminotransferase e creatinoquinase, lactato e o perfil oxidativo através de espécies reativas ao oxigênio, espécies reativas ao ácido tiobarbitúrico, catalase, superóxido dismutase e ácido úrico. Os dados foram estudados através de uma análise de variância associada ao teste de Tukey, considerando-se significativo para P ≤ 0,05, além de uma correlação de Spearman, considerando-se significativo P ≤ 0,05. As elevações significativas de CK e lactato em T1 associadas à manutenção dos níveis dos indicadores oxidativos em T1 e T18 refletiram um significativo esforço muscular e a discreta mobilização de parâmetros oxidativos que foi compensada pelas defesas antioxidantes, visto que os animais apresentaram-se recuperados após T18. Não houve danos a nível celular e os equinos mostraram uma condição muscular e antioxidante apta ao exercício.
Publication Date: 2024-10-11 PubMed ID: 39439980PubMed Central: PMC11495231DOI: 10.29374/2527-2179.bjvm005823Google Scholar: Lookup
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  • Journal Article

Summary

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The research investigates muscle and oxidative changes in Criollo horses engaged in the long loop rodeo sport, detailing how physical exertion influences these horses’ muscle function and oxidative profile, and that any impact noted is sufficiently managed by the animals’ antioxidant defenses.

Research Methodology

  • The subjects of this study were Criollo horses from Brazil, a breed popular in the long loop rodeo sport. This study’s primary aim was to determine the changes in muscle and oxidative functions following the rigorous exercises involved in the sport.
  • Data collected included the frequency of exercise, distance covered, average duration time, and number of runs. Blood samples were also taken before the tournament, at the end of the final day, and 18-24 hours following the tournament’s conclusion.
  • To analyze muscle function, the levels of lactate and the enzymes aspartate aminotransferase and creatine kinase were evaluated. For oxidative stress, species reactive to oxygen, species reactive to thiobarbituric acid, catalase, superoxide dismutase, and uric acid were assessed.
  • Collected data were scrutinized via variance analysis paired with the Tukey test (for statistical significance set at P ≤ 0.05) and Spearman’s correlation (also at P ≤ 0.05).

Research Findings

  • The study collected significant data on the increase in creatine kinase and lactate in the horses after engaging in the physically demanding rodeo sport (T1). This indicated a real muscular effort during this period.
  • Meanwhile, the oxidative indicator levels, which help measure oxidative stress, remained consistent in T1 and T18, showing only minimal shifts in oxidative parameters.
  • The study found this slight change was offset by the horses’ antioxidant defenses, as the animals demonstrated recovery after the T18 period.
  • Importantly, the research noted no cell-level damage in evaluated horses, suggesting that the Criollo horses maintain good muscle and antioxidant conditions even after strenuous physical exercise in the long loop rodeo.

Conclusion

  • This study thus unveiled that the physical demands of the long loop rodeo do lead to an increase in certain muscle function indicators in Criollo horses. However, these changes do not cause cell damage, thanks to the animals’ antioxidant compensating mechanisms.
  • The importance of the research lies in showing how Criollo horses manage oxidative stress and maintain muscle function despite the rigorous exercise, making them a suitable breed for such physically challenging sports.

Cite This Article

APA
Pereira AT, Pozzobon R, Dos Anjos BL, Chaves LT, Smilgys ÉC, Thoma AJS, Lunkes VL, de Oliveira JS, de Andrade CM, Soares JCM. (2024). Assessing muscle function and oxidative profile in criollo horses participating in long loop rodeo before and after exercise. Braz J Vet Med, 46, e005823. https://doi.org/10.29374/2527-2179.bjvm005823

Publication

ISSN: 2527-2179
NlmUniqueID: 9918435088106676
Country: Brazil
Language: English
Volume: 46
Pages: e005823

Researcher Affiliations

Pereira, Andrielli Trentim
  • Veterinarian, Programa de Pós-Graduação em Ciência Animal, Departamento de Clínica de Grandes Animais (DCGA), Hospital Veterinário Universitário (HUVet), Universidade Federal do Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
Pozzobon, Ricardo
  • Veterinarian, DSc. DCGA, Hospital Universitário Veterinário HVU, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Dos Anjos, Bruno Leite
  • Veterinarian, DSc. Departamento de Patologia Veterinária (DPV), HUVet, UNIPAMPA, Uruguaiana, RS, Brazil.
Chaves, Leonardo Trentin
  • Undergraduate in Veterinary Medicine, HUVet, UNIPAMPA, Uruguaiana, RS, Brazil.
Smilgys, Érika Carla
  • Undergraduate in Veterinary Medicine, HVU, UFSM, Santa Maria, RS, Brazil.
Thoma, Ana Júlia Santos
  • Undergraduate in Veterinary Medicine, HVU, UFSM, Santa Maria, RS, Brazil.
Lunkes, Vinícius Leobet
  • Veterinarian, MSc. Departamento de Patologia Clínica Veterinária (DPCV), HVU, UFSM, Santa Maria, RS, Brazil.
de Oliveira, Juliana Sorraila
  • Veterinarian, DSc. DPCV, HVU, UFSM, Santa Maria, RS, Brazil.
de Andrade, Cinthia Melazzo
  • Veterinarian, DSc. DPCV, HVU, UFSM, Santa Maria, RS, Brazil.
Soares, Júlio Cesar Mendes
  • Veterinarian, DSc. DCGA, UFSM, HVU, Santa Maria, RS, Brazil.

Conflict of Interest Statement

Conflict of interests: ATP, RP, BLA, LTC, ECS, AJST, VLL, JSO, CMA and JCMS – No conflict of interest.

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