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Home / Equine Research Bank / Anim Biosci / Assessment of genetic diversity using microsatellite markers...
Animal bioscience2021; 34(9); 1460-1465; doi: 10.5713/ab.20.0860

Assessment of genetic diversity using microsatellite markers to compare donkeys (Equus asinus) with horses (Equus caballus).

Abstract: The study aimed to evaluate the diversity of donkey populations by comparing with the diversity of Thoroughbred and Jeju Halla horses; identified breeding backgrounds can contribute to management and conservation of donkeys in South Korea. Methods: A total of 100 horse (50 Thoroughbreds and 50 Jeju Halla horses) and 79 donkeys samples were genotyped with 15 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3, and VHL20), to identify genetic diversity and relationships among horses and donkeys. Results: The observed number of alleles per locus ranged from 1 (ASB17, HMS1) to 14 (AHT5), with a mean value of 4.87, 8.00, and 5.87 in Thoroughbreds, Jeju Halla horses, and donkeys, respectively. Of the 15 markers, AHT4, AHT5, ASB23, CA425, HMS2, HMS3, HTG4, HTG10, and LEX3 loci had relatively high polymorphism information content (PIC) values (PIC>0.5) in these three populations. Mean levels of genetic variation were HE = 0.6721 and HO = 0.6600 in Thoroughbreds, HE = 0.7898 and HO = 0.7100 in Jeju Halla horses, and HE = 0.5635 and HO = 0.4861 in donkeys. Of the 15 loci in donkeys, three loci had negative inbreeding coefficients (FIS), with a moderate mean FIS (0.138). The FIS estimate for the HTG4 marker was highest (0.531) and HMS6 marker was lowest (-0.001). The total probability of exclusion value of 15 microsatellite loci was 0.9996 in donkeys. Conclusions: Genetic cluster analysis showed that the genetic relationship among 79 donkeys was generally consistent with pedigree records. Among the three breeds, donkeys and Thoroughbred horses formed clearly different groups, but the group of Jeju Halla horses overlapped with that of Thoroughbred horses, suggesting that the loci would be suitable for donkey parentage testing. Therefore, the results of this study are a valid tool for genetic study and conservation of donkeys.
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Publication Date: 2021-04-23 PubMed ID: 33902168PubMed Central: PMC8495341DOI: 10.5713/ab.20.0860Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the genetic diversity of donkeys in comparison to Thoroughbred and Jeju Halla horses. The researchers used microsatellite markers to examine the genetic cloistering of samples.

Methodology

  • For the study, samples were collected from 100 horses (comprising of 50 Thoroughbreds and 50 Jeju Halla horses) and 79 donkeys.
  • These samples were genotyped using 15 specific microsatellite markers, namely AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3, and VHL20.
  • This data was processed to understand the genetic diversity and the relational mapping between the horses and the donkeys.

Findings

  • Analysis of the observed number of alleles per locus showed a range from 1 (ASB17, HMS1) to 14 (AHT5).
  • The average values were 4.87 in Thoroughbreds, 8.00 in Jeju Halla horses, and 5.87 in donkeys.
  • Of the 15 markers used, AHT4, AHT5, ASB23, CA425, HMS2, HMS3, HTG4, HTG10, and LEX3 demonstrated high polymorphism information content, reflecting their value in diversity measurement.
  • Genetic variation mean values were as follows: HE = 0.6721 and HO = 0.6600 in Thoroughbreds, HE = 0.7898 and HO = 0.7100 in Jeju Halla horses, and HE = 0.5635 and HO = 0.4861 in donkeys.
  • Analysis of the inbreeding coefficients (FIS) revealed that three of the 15 donkey loci were negative, rendering an overall moderate mean of FIS at 0.138. The highest FIS measure was given by the HTG4 marker (0.531) and the lowest by the HMS6 marker (-0.001).
  • The total probability of exclusion value of these 15 microsatellite marks was 0.9996 in donkeys, demonstrating the effectiveness of these markers in donkey genetic studies.

Conclusion

  • Genetic cluster analysis showed consistency between the genetic relationships among the 79 donkeys and the pedigree records.
  • Clear distinction was observed between the genetic groups of donkeys and Thoroughbred horses. However, overlap was noted in the groups between Jeju Halla horses and Thoroughbreds.
  • This study’s results strongly suggest potential for these loci in donkey parentage testing. The findings would prove useful in establishing better management and conservation measures for South Korean donkeys.

Cite This Article

APA
Kim SM, Yun SW, Cho GJ. (2021). Assessment of genetic diversity using microsatellite markers to compare donkeys (Equus asinus) with horses (Equus caballus). Anim Biosci, 34(9), 1460-1465. https://doi.org/10.5713/ab.20.0860

Publication

Animal bioscience
ISSN: 2765-0189
NlmUniqueID: 101774366
Country: Korea (South)
Language: English
Volume: 34
Issue: 9
Pages: 1460-1465

Researcher Affiliations

Kim, Su Min
  • College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
Yun, Sung Wook
  • College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, Daegu 41566, Korea.
Cho, Gil Jae
  • College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
  • College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, Daegu 41566, Korea.

Grant Funding

  • 2020R1I1A3067905 / National Research Foundation of Korea
  • Ministry of Education, Science and Technology

Conflict of Interest Statement

. We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

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Citations

This article has been cited 2 times.
  1. Wang T, Liu Z, Shi X, Zhang Z, Li Y, Huang B, Ren W, Wang X, Wang C, Chai W. An investigation of genetic diversity in three Dezhou donkey original breeding farms.. Sci Rep 2023 Jul 11;13(1):11203.
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  2. Park CS, Lee SY, Cho GJ. Evaluation of recent changes in genetic variability in Thoroughbred horses based on microsatellite markers parentage panel in Korea.. Anim Biosci 2022 Apr;35(4):527-532.
    doi: 10.5713/ab.21.0272pubmed: 34530514google scholar: lookup
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