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Home / Equine Research Bank / BMC Ecol Evol / Biological response to Przewalski’s horse reintroducti...
BMC ecology and evolution2024; 24(1); 61; doi: 10.1186/s12862-024-02252-z

Biological response to Przewalski’s horse reintroduction in native desert grasslands: a case study on the spatial analysis of ticks.

Abstract: Reintroduction represents an effective strategy for the conservation of endangered wildlife, yet it might inadvertently impact the native ecosystems. This investigation assesses the impact of reintroducing endangered Przewalski's horses into the desert grassland ecosystem of the Kalamaili Nature Reserve (KNR), particularly its effect on the spatial distribution of ticks. In a 25 km core area of Przewalski's horse distribution, we set up 441 tick sampling sites across diverse habitats, including water sources, donkey trails, and grasslands, recording horse feces and characteristics to analyze the occurrence rate of ticks. Additionally, we gathered the data of 669 fresh feces of horses. To evaluate the spatial dynamics between these feces and ticks, we used methods such as Fixed Kernel Estimation (FKE), Moran's I spatial autocorrelation index, and Generalized Linear Models (GLM). Results: The dominant species of ticks collected in the core area were adult Hyalomma asiaticum (91.36%). Their occurrence rate was higher near donkey trails (65.99%) and water sources (55.81%), particularly in areas with the fresh feces of Przewalski's horses. The ticks' three risk areas, as defined by FKE, showed significant overlap and positive correlation with the distribution of Przewalski's horses, with respective overlap rates being 90.25% in high risk, 33.79% in medium risk, and 23.09% in low risk areas. Moran's I analysis revealed a clustering trend of the fresh feces of Przewalski's horses in these areas. The GLM confirmed a positive correlation between the distribution of H. asiaticum and the presence of horse fresh feces, alongside a negative correlation with the proximity to water sources and donkey trails. Conclusions: This study reveals the strong spatial correlation between Przewalski's horses and H. asiaticum in desert grasslands, underlining the need to consider interspecific interactions in wildlife reintroductions. The findings are crucial for shaping effective strategies of wildlife conservation and maintaining ecological balance.
© 2024. The Author(s).
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Publication Date: 2024-05-11 PubMed ID: 38734637PubMed Central: PMC11088120DOI: 10.1186/s12862-024-02252-zGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the impact of reintroducing endangered Przewalski’s horses into an ecosystem, focusing on how it affects the distribution of ticks in the Kalamaili Nature Reserve.

Introduction and Study Method

  • The article discusses an ecological study conducted in the Kalamaili Nature Reserve (KNR), where endangered Przewalski’s horses were reintroduced to determine the effect on the local tick population.
  • A 25-kilometer region was designated as the core area of horse distribution, and within this area, 441 different sites were set up to collect and analyze ticks.
  • The collection sites were strategically placed across varied habitats, including water sources, donkey trails, and grasslands. Additionally, data from 669 fresh samples of horse feces were collected and analyzed to assess their relationship with the ticks.

Results Analysis

  • The most common species of tick found in the core area were the adult Hyalomma asiaticum, making up 91.36% of the tick population in the surveyed region.
  • These ticks were found in higher concentration close to donkey trails (65.99%) and water sources (55.81%), especially in places where fresh Przewalski’s horse feces were present.
  • Statistical methods such as Fixed Kernel Estimation (FKE), Moran’s I spatial autocorrelation index, and Generalized Linear Models (GLM) were used to evaluate the spatial relationship between horse feces and ticks.
  • The identified risk areas for tick concentration, as determined by FKE, showed significant overlap and positive correlation with where Przewalski’s horses were found. Notably, the rate of overlap for high risk areas was 90.25%.

Conclusion and Recommendations

  • The findings of the study indicate a strong spatial correlation between the reintroduced Przewalski’s horses and the population of Hyalomma asiaticum ticks in the desert grasslands.
  • Moran’s I analysis identified a clustering trend of the Przewalski’s horses’ fresh feces in these high tick concentration areas.
  • The study concluded that there is a positive correlation between the distribution of H. asiaticum ticks and the presence of fresh horse feces. Furthermore, a negative correlation was found between the ticks and proximity to water sources and donkey trails.
  • This discovery is essential for creating effective wildlife conservation strategies. It demonstrates the need to consider interspecies interactions when reintroducing wildlife to preserve ecological balance.

Cite This Article

APA
Zhang Y, Liu J, Zhang K, Wang A, Sailikebieke D, Zhang Z, Ao T, Yan L, Zhang D, Li K, Huang H. (2024). Biological response to Przewalski’s horse reintroduction in native desert grasslands: a case study on the spatial analysis of ticks. BMC Ecol Evol, 24(1), 61. https://doi.org/10.1186/s12862-024-02252-z

Publication

BMC ecology and evolution
ISSN: 2730-7182
NlmUniqueID: 101775613
Country: England
Language: English
Volume: 24
Issue: 1
Pages: 61
PII: 61

Researcher Affiliations

Zhang, Yu
  • School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China.
Liu, Jiawei
  • School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China.
Zhang, Ke
  • Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, China.
Wang, Anqi
  • School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China.
Sailikebieke, Duishan
  • Xinjiang Fuyun County Kizillike Township Agricultural Development Center, Altay, China.
Zhang, Zexin
  • Tongliao Forestry Pest Control Station, Tongliao, China.
Ao, Tegen
  • Tongliao Control and Quarantine Station of Forest Pest, Tongliao, China.
Yan, Liping
  • School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China.
Zhang, Dong
  • School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China.
Li, Kai
  • School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China. jiujiu@bjfu.edu.cn.
Huang, Heqing
  • Chongqing Academy of Environmental Science, Chongqing, China. jiaoxiaoqing929@163.com.

MeSH Terms

  • Animals
  • Horses
  • Grassland
  • Conservation of Natural Resources / methods
  • Spatial Analysis
  • Feces / parasitology
  • Feces / chemistry
  • Desert Climate
  • Ixodidae / physiology
  • Endangered Species

Grant Funding

  • 2021xjkk1201 / the Investigation of natural protected areas and scientific investigation of potential areas of National Parks in Xinjiang
  • 2024-HXFWBH-LK-01 / the Parasite Control Project of the Forestry and Grassland Bureau of Xinjiang

Conflict of Interest Statement

The authors declare no competing interests.

References

This article includes 69 references
  1. Maestre FT, Eldridge DJ, Soliveres S, Kéfi S, Delgado-Baquerizo M, Bowker MA. Structure and functioning of dryland ecosystems in a changing world. Annu Rev Ecol Syst 2016;47:215–237.
    doi: 10.1146/annurev-ecolsys-121415-032311pmc: PMC5321561pubmed: 28239303google scholar: lookup
  2. Kang L, Han X, Zhang Z, Sun OJ. Grassland ecosystems in China: review of current knowledge and research advancement. Philos T R Soc B 2007;362:997–1008.
    doi: 10.1098/rstb.2007.2029pmc: PMC2435566pubmed: 17317645google scholar: lookup
  3. Barbolini N, Woutersen A, Dupont-Nivet G, Silvestro D, Tardif D, Coster PMC. Cenozoic evolution of the steppe-desert biome in Central Asia. Sci Adv 2020;6:eabb8227.
    doi: 10.1126/sciadv.abb8227pmc: PMC7546705pubmed: 33036969google scholar: lookup
  4. Zhang Y, Tariq A, Hughes AC, Hong D, Wei F, Sun H. Challenges and solutions to biodiversity conservation in arid lands. Sci Total Environ 2023;857:159695.
    doi: 10.1016/j.scitotenv.2022.159695pubmed: 36302433google scholar: lookup
  5. Van Dierendonck MC, de WallisVries MF. Ungulate Reintroductions: Experiences with the Takhi or Przewalski Horse (Equus ferus przewalskii) in Mongolia. Conserv Biol 1996;10:728–40.
    doi: 10.1046/j.1523-1739.1996.10030728.xgoogle scholar: lookup
  6. Seddon PJ, Armstrong DP, Maloney RF. Developing the science of reintroduction biology. Conserv Biol 2007;21:303–312.
    doi: 10.1111/j.1523-1739.2006.00627.xpubmed: 17391180google scholar: lookup
  7. Wang Y, Chu HJ, Han LL, Tao YS, Bu L, Liu Z. Factors affecting the home range of reintroduced Equus przewalskii in the Mt.Kalamaili Ungulate Nature Reserve. Acta Ecol Sin 2016;02:545–553.
  8. Xia CJ, Cao J, Zhang HF, Gao XY, Yang WK, Blank D. Reintroduction of Przewalski’s horse (Equus przewalskii) in Xinjiang, China: The status and experience. Biol Conserv 2014;177:142–147.
    doi: 10.1016/j.biocon.2014.06.021google scholar: lookup
  9. Jiang Z, Zong H. Reintroduction of the Przewalski's horse in China: status quo and outlook. Nat Conserv Res 2019;4:15–22.
    doi: 10.24189/ncr.2019.045google scholar: lookup
  10. Huang HQ, Zhang K, Zhang BR, Liu SH, Chu HJ, Qi YJ. Analysis on the relationship between winter precipitation and the annual variation of horse stomach fly community in arid desert steppe, Northwest China (2007–2019). Integr Zool 2022;17:128–138.
    doi: 10.1111/1749-4877.12578pmc: PMC9291967pubmed: 34254452google scholar: lookup
  11. Huang HQ, Zhang K, Shao CL, Wang C, Ente MK, Wang ZB. Spatial distribution of Gasterophilus pecorum (Diptera) eggs in the desert steppe of the Kalamaili Nature Reserve (Xinjiang, China). BMC Ecol Evol 2021;21:169.
    doi: 10.1186/s12862-021-01897-4pmc: PMC8422714pubmed: 34488639google scholar: lookup
  12. Zhang K, Zhang Y, Wang C, Ge Y, Chu HJ, Zhang YB. Distribution characteristics of epidemic focus of Gasterophilus pecorum (Diptera: Gasterophilidae) in the core habitat of released Przewalski's horses. Acta Ecol Sin 2023;43:5840–5849.
  13. Huang HQ, Zhang BR, Chu HJ, Zhang D, Li K. Gasterophilus (Diptera, Gasterophilidae) infestation of equids in the Kalamaili Nature Reserve, China. Parasite 2016;23:36.
    doi: 10.1051/parasite/2016036pmc: PMC5018932pubmed: 27593434google scholar: lookup
  14. Zhang K, Huang HQ, Zhou R, Zhang BR, Wang C, Ente MK. The impact of temperature on the life cycle of Gasterophilus pecorum in northwest China. Parasit Vectors 2021;14:129.
    doi: 10.1186/s13071-021-04623-7pmc: PMC7923332pubmed: 33648570google scholar: lookup
  15. Valcárcel F, González J, González MG, Sánchez M, Tercero JM, Elhachimi L. Comparative Ecology of Hyalomma lusitanicum and Hyalomma marginatum Koch, 1844 (Acarina: Ixodidae). Insects 2020;11:303.
    doi: 10.3390/insects11050303pmc: PMC7290797pubmed: 32414220google scholar: lookup
  16. Yu X, Ye RY, Gong ZD. The ticks fauna of Xinjiang. Urumqi: Xinjiang Scientific, Technological and Medical Publishing House; 1997. p. 8–74. (In Chinese).
  17. Guglielmone AA, Robbins RG, Apanaskevich DA, Petney TN, Estrada-Peña A, Horak IG. The hard ticks of the world. Springer, Dordrecht. 2014;10:978–994.
  18. Zhang YK, Zhang XY, Liu JZ. Ticks (Acari: Ixodoidea) in China: Geographical distribution, host diversity, and specificity. Arch Insect Biochem 2019;10:e21544.
    doi: 10.1002/arch.21544pmc: PMC6850514pubmed: 30859631google scholar: lookup
  19. Sheng J, Jiang M, Yang M, Bo X, Zhao S, Zhang Y. Tick distribution in border regions of Northwestern China. Ticks Tick-borne Dis 2019;10:665–669.
    doi: 10.1016/j.ttbdis.2019.02.011pubmed: 30833199google scholar: lookup
  20. Wang YZ, Mu LM, Zhang K, Yang MH, Zhang L, Du JY. A broad-range survey of ticks from livestock in Northern Xinjiang: changes in tick distribution and the isolation of Borrelia burgdorferi sensu stricto. Parasite Vector 2015;8:449.
    doi: 10.1186/s13071-015-1021-0pmc: PMC4560164pubmed: 26337627google scholar: lookup
  21. Bonnet SI, Vourc’pH G, Raffetin A, Falchi A, Figoni J, Fite J. The control of Hyalomma ticks, vectors of the Crimean-Congo hemorrhagic fever virus: Where are we now and where are we going?. PLoS Negl Trop Dis 2022;16:e0010846.
    doi: 10.1371/journal.pntd.0010846pmc: PMC9671348pubmed: 36395110google scholar: lookup
  22. Zakham F, Albalawi AE, Alanazi AD, Truong Nguyen P, Alouffi AS, Alaoui A. Viral RNA metagenomics of Hyalomma ticks collected from dromedary camels in Makkah Province. Saudi Arabia. Viruses 2021;13:1396.
    doi: 10.3390/v13071396pmc: PMC8310382pubmed: 34372602google scholar: lookup
  23. Chen Z, Yu ZJ, Yang XJ, Zheng HY, Liu JZ. The life cycle of Hyalomma asiaticum kozlovi Olenev, 1931 (Acari: Ixodidae) under laboratory conditions. Vet Parasitol 2009;160:134–137.
    doi: 10.1016/j.vetpar.2008.10.028pubmed: 19062189google scholar: lookup
  24. Hosseini-Chegeni A, Hosseini R, Tavakoli M, Telmadarraiy Z, Abdigoudarzi M. The Iranian Hyalomma (Acari: Ixodidae) with a key to the identification of male species. Persian J Acarol 2013;2:503–29.
  25. Perveen N, Bin Muzaffar S, Al-Deeb MA. Population dynamics of Hyalomma dromedarii on camels in the United Arab Emirates. Insects 2020;11:320.
    doi: 10.3390/insects11050320pmc: PMC7291271pubmed: 32456119google scholar: lookup
  26. Rojas-Jaimes J, Lindo-Seminario D, Correa-Núñez G, Diringer B. Characterization of the bacterial microbiome of Rhipicephalus (Boophilus) microplus collected from Pecari tajacu “Sajino” Madre de Dios. Peru. Sci Rep 2021;11:6661.
    doi: 10.1038/s41598-021-86177-3pmc: PMC7988070pubmed: 33758359google scholar: lookup
  27. Yang XH, Zhao YQ, Chuai X, Cao Q, Meng H, Liu JZ. The life cycle of Hyalomma scupense (Acari: Ixodidae) under laboratory conditions. Ticks Tick Borne Dis 2022;13:102019.
    doi: 10.1016/j.ttbdis.2022.102019pubmed: 35963189google scholar: lookup
  28. Estrada-Peña A, Bouattour A, Camicas JL, Walker AR. Ticks of domestic animals in the Mediterranean region. 2004. pp. 21–22.
  29. Leal B, Zamora E, Fuentes A, Thomas DB, Dearth RK. Questing by tick larvae (Acari: Ixodidae): a review of the influences that affect off-host survival. Ann Entomol Soc Am 2020;113:425–438.
    doi: 10.1093/aesa/saaa013pmc: PMC7677832pubmed: 33244354google scholar: lookup
  30. Orkun Ö, Çakmak A, Nalbantoğlu S, Karaer Z. Turkey tick news: A molecular investigation into the presence of tick-borne pathogens in host-seeking ticks in Anatolia; Initial evidence of putative vectors and pathogens, and footsteps of a secretly rising vector tick, Haemaphysalis parva. Ticks Tick-borne Dis 2020;11:101373.
    doi: 10.1016/j.ttbdis.2020.101373pubmed: 31964592google scholar: lookup
  31. Zhang YJ, Cao QS, Rubenstein DI, Zang S, Songer M, Leimgruber P. Water use patterns of sympatric Przewalski’s horse and Khulan: Interspecific comparison reveals niche differences. PLoS One 2015;10:e0132094.
    doi: 10.1371/journal.pone.0132094pmc: PMC4498657pubmed: 26161909google scholar: lookup
  32. Chen JL, Hu DF, Li K, Cao J, Meng YP, Cui YY. The diurnal feeding behavior comparison between the released and captive adult female Przewalski’s horse (Equus przewalskii) in summer. Acta Ecol Sin 2008;28:1104–1108.
  33. Hu DN, Wang C, Ente M, Zhang K, Zhang D, Li X. Assessment of adaptation status of reintroduced Equus Przewalskii based on comparative analysis of fecal bacteria with those of captive E. Przewalskii, domestic horse and mongolian wild ass. Animals 2022;12:2874.
    doi: 10.3390/ani12202874pmc: PMC9598124pubmed: 36290262google scholar: lookup
  34. Hudson P, Greenman J. Competition mediated by parasites: biological and theoretical progress. Trends Ecol Evol 1998;13:387–390.
    doi: 10.1016/S0169-5347(98)01475-Xpubmed: 21238357google scholar: lookup
  35. Keesing F, Allan BF, Young TP, Ostfeld RS. Effects of wildlife and cattle on tick abundance in central Kenya. Ecol Appl 2013;23:1410–1418.
    doi: 10.1890/12-1607.1pubmed: 24147412google scholar: lookup
  36. Slabach BL, McKinney A, Cunningham J, Hast JT, Cox JJ. A survey of tick species in a recently reintroduced Elk (Cervus canadensis) population in Southeastern Kentucky, USA, with potential implications for interstate translocation of zoonotic disease vectors. J Wildlife Dis 2018;54:366–370.
    doi: 10.7589/2017-06-135pubmed: 29286258google scholar: lookup
  37. Matsuyama H, Taira M, Suzuki MJ. Regional scale distribution of tick is associated with wildlife distribution on the Boso Peninsula. Central Japan Mammal Study 2022;47:265–273.
  38. Thulin CG, Röcklinsberg H. Ethical considerations for wildlife reintroductions and rewilding. Front Vet Sci 2020;7:163.
    doi: 10.3389/fvets.2020.00163pmc: PMC7146822pubmed: 32318586google scholar: lookup
  39. Jørgensen D. Conservation implications of parasite co-reintroduction. Conser Biol 2015;29:602–604.
    doi: 10.1111/cobi.12421pubmed: 25370175google scholar: lookup
  40. Zang S, Cao Q, Alimujiang K, Liu SH, Zhang YJ, Hu DF. Food patch particularity and forging strategy of reintroduced Przewalski’s horse in North Xinjiang. China. Turk J Zool 2017;41:924–930.
    doi: 10.3906/zoo-1509-9google scholar: lookup
  41. Elati K, Benyedem H, Fukatsu K, Hoffmann-Köhler P, Mhadhbi M, Bakırcı S. In vitro feeding of all life stages of two-host Hyalomma excavatum and Hyalomma scupense and three-host Hyalomma dromedarii ticks. Sci Rep 2024;14:444.
    doi: 10.1038/s41598-023-51052-wpmc: PMC10764919pubmed: 38172407google scholar: lookup
  42. Zhang K, Zhou R, Huang HQ, Ma W, Qi YJ, Li BL. Host feces, olfactory beacon guiding aggregation of intestinal parasites Gasterophilus pecorum (Diptera: Gasterophilidae). Parasitol Res 2022;121:2601–2613.
    doi: 10.1007/s00436-022-07577-6pubmed: 35788769google scholar: lookup
  43. Zhou R, Yang JM, Zhang K, Qi YJ, Ma W, Wang ZB. Analysis of volatiles from feces of released Przewalski's horse (Equus przewalskii) in Gasterophilus pecorum (Diptera: Gasterophilidae) spawning habitat. Sci Rep 2021;11:15671.
    doi: 10.1038/s41598-021-95162-9pmc: PMC8329074pubmed: 34341455google scholar: lookup
  44. Sonenshine DE, Roe RM. Biology of ticks volume 1 (Vol. 1). Oxford University Press, USA. 2014. p. 28–34, 74–98.
  45. Apanaskevich DA, Horak IG. The genus Hyalomma Koch, 1844: v. re-evaluation of the taxonomic rank of taxa comprising the H. (Euhyalomma) marginatum koch complex of species (Acari: Ixodidae) with redescription of all parasitic stages and notes on biology. Int J Acarol 2008;34:13–42.
    doi: 10.1080/01647950808683704google scholar: lookup
  46. Bakheit MA, Latif AA, Vatansever Z, Seitzer U, Ahmed J. The Huge Risks Due to Hyalomma Ticks. In: Mehlhorn H, editor. Arthropods as Vectors of Emerging Diseases. Berlin: Heidelberg, Springer, Berlin Heidelberg; 2012. pp. 167–194.
  47. Qviller L, Viljugrein H, Loe LE, Meisingset EL, Mysterud A. The influence of red deer space use on the distribution of Ixodes ricinus ticks in the landscape. Parasit Vectors 2016;9:545.
    doi: 10.1186/s13071-016-1825-6pmc: PMC5064927pubmed: 27737695google scholar: lookup
  48. Miguel E, Boulinier T, Garine-Wichatitsky M, Caron A, Fritz H, Grosbois V. Characterising African tick communities at a wild-domestic interface using repeated sampling protocols and models. Acta Trop 2014;138:5–14.
    doi: 10.1016/j.actatropica.2014.05.019pubmed: 24905293google scholar: lookup
  49. Legendre P, Fortin MJ. Spatial pattern and ecological analysis. Vegetatio 1989;80:107–138.
    doi: 10.1007/BF00048036google scholar: lookup
  50. Chen Y. New approaches for calculating Moran’s index of spatial autocorrelation. PLoS One 2013;8:e68336.
    doi: 10.1371/journal.pone.0068336pmc: PMC3709922pubmed: 23874592google scholar: lookup
  51. Almberg ES, Cross PC, Dobson AP, Smith DW, Hudson PJ. Parasite invasion following host reintroduction: a case study of Yellowstone's wolves. Philos Trans R Soc B: Biol Sci 2012;367:2840–2851.
    doi: 10.1098/rstb.2011.0369pmc: PMC3427562pubmed: 22966139google scholar: lookup
  52. Anne EG, Alison NH, Rachel LK, Richard NR, Hannah S, Lynne M. Managing grassland for wildlife: the effects of rotational burning on tick presence and abundance in African savannah habitat. Wildlife Biol 2017;1:1–8.
  53. Bunnell T, Hanisch K, Hardege JD, Breithaupt T. The Fecal Odor of Sick Hedgehogs (Erinaceus europaeus) Mediates olfactory attraction of the Tick Ixodes hexagonus. J Chem Ecol 2011;2011(37):340–347.
    doi: 10.1007/s10886-011-9936-1pubmed: 21445567google scholar: lookup
  54. King S, Gurnell J. Scent-marking behaviour by stallions: An assessment of function in a reintroduced population of Przewalski horses (Equus ferus przewalskii). J Zool 2006;272:30–36.
    doi: 10.1111/j.1469-7998.2006.00243.xgoogle scholar: lookup
  55. Da Re D, De Clercq EM, Tordoni E, Madder M, Rousseau R, Vanwambeke SO. Looking for ticks from space: using remotely sensed spectral diversity to assess Amblyomma and Hyalomma Tick Abundance. Remote Sens 2019;11:770.
    doi: 10.3390/rs11070770google scholar: lookup
  56. Harlan HJ, Foster WA. Micrometeorologic Factors Affecting Field Host-Seeking Activity of Adult Dermacentor variabilis (Acari: Ixodidae). J Med Entomol 1990;27:471–479.
    doi: 10.1093/jmedent/27.4.471pubmed: 2388223google scholar: lookup
  57. Schulze TL, Jordan RA, Hung RW. Potential Effects of Animal Activity on the Spatial Distribution of Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae). Environ Entomol 2001;30:568–577.
    doi: 10.1603/0046-225X-30.3.568google scholar: lookup
  58. Klimov VV. Spatial-ethological organization of the herd of Przewalski horses (Equus przewalskii) in Askania-Nova. Appl Anim Behav Sci 1988;21:99–115.
    doi: 10.1016/0168-1591(88)90103-7google scholar: lookup
  59. Kaczensky P, Burnik Šturm M, Sablin MV, Voigt CC, Smith S, Ganbaatar O. Stable isotopes reveal diet shift from pre-extinction to reintroduced Przewalski’s horses. Sci Rep 2017;7:5950.
    doi: 10.1038/s41598-017-05329-6pmc: PMC5519547pubmed: 28729625google scholar: lookup
  60. Zhang XC, Shao CL, Ge Y, Chen C, Xu WX, Yang WK. Suitable summer habitat of the khulan in the Mt. Kalamaili Ungulate Nature Reserve and estimation of its population. Chin J Appl Ecol 2020;31:2993–3004.
    pubmed: 33345500
  61. Chu HJ, Jiang ZG, Ge Y, Jiang F, Tao YS, Wang C. Population densities and number of khulan and goitred gazelle in Mt. Kalamaili Ungulate Nature Reserve. Biodivers Sci 2009;17:414–422.
    doi: 10.3724/SP.J.1003.2009.09001google scholar: lookup
  62. Kaczensky P, Ganbaatar O, Walzer HVW. Resource selection by sympatric wild equids in the Mongolian Gobi. J Appl Ecol 2010;45:1762–1769.
    doi: 10.1111/j.1365-2664.2008.01565.xgoogle scholar: lookup
  63. Kie JG. A rule-based ad hoc method for selecting a bandwidth in kernel home-range analyses. Anim Biotelem 2013;1:13.
    doi: 10.1186/2050-3385-1-13google scholar: lookup
  64. Silva I, Crane M, Suwanwaree P, Strine C, Goode M. Using dynamic Brownian Bridge Movement Models to identify home range size and movement patterns in king cobras. PLoS One 2018;13:e0203449.
    doi: 10.1371/journal.pone.0203449pmc: PMC6143228pubmed: 30226846google scholar: lookup
  65. Wauters LA, Preatoni DG, Molinari A, Tosi G. Radio-tracking squirrels: Performance of home range density and linkage estimators with small range and sample size. Ecol Modell 2007;202:333–344.
    doi: 10.1016/j.ecolmodel.2006.11.001google scholar: lookup
  66. Turghan MA, Jiang Z, Niu Z. An update on status and conservation of the Przewalski’s Horse (Equus ferus przewalskii): captive breeding and reintroduction projects. Animals 2022;12:3158.
    doi: 10.3390/ani12223158pmc: PMC9686875pubmed: 36428386google scholar: lookup
  67. Kołodziej-Sobocińska M. Factors affecting the spread of parasites in populations of wild European terrestrial mammals. Mamm Res 2019;649:301–318.
    doi: 10.1007/s13364-019-00423-8google scholar: lookup
  68. Kwak ML, Heath ACG, Cardoso P. Methods for the assessment and conservation of threatened animal parasites. Biol Conserv 2020;248:108696.
    doi: 10.1016/j.biocon.2020.108696google scholar: lookup
  69. Li Z, Beauchamp G, Mooring MS. Relaxed selection for tick-defense grooming in Père David’s deer?. Biol Conserv 2014;178:12–18.
    doi: 10.1016/j.biocon.2014.06.026google scholar: lookup

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