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Home / Equine Research Bank / J Virol / Characterization of a retravirus isolated from squirrel monk...
Journal of virology1977; 23(2); 384-393; doi: 10.1128/JVI.23.2.384-393.1977

Characterization of a retravirus isolated from squirrel monkeys.

Abstract: A new retravirus (SMRV) isolated from a squirrel monkey, Saimiri sciureus, has an Mg2+-dependen reverse transcriptase and a buoyant density of 1.17 g/cm3 in sucrose and 1.21 g/cm3 in cesium chloride, similar to the mouse mammary tumor virus and the Mason-Pfizer monkey virus. The polypeptide patter of SMRV as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was distinct from the reported polypeptide patterns of known retraviruses. Four major polypeptides of molecular weights 40,000, 20,000, 14,000 and 8,000 were resolved in virus propagated in human, mink, and canine cells. In A204 human rhabdomyosarcoma cells, a protein of 73,000 daltons (gp73) represented the major viral glycoprotein as determined by [3H]glucosamine labeling. Additional proteins were also observed, but their presence depended on the cell type in which the virus was propagated. In both species-and interspecies-specific assays, no antigenic relatedness was observed between SMRV and Mason-Pfizer monkey virus, mouse mammary tumor virus, baboon endogenous virus (BaLV), woolly monkey virus (SSV-1), murine leukemia virus, endogenous feline type C virus (RD-114), bovine leukemia virus, and equine infectious anemia virus. These findings indicate that SMRV represents a new retravirus and the first isolate from a New World monkey.
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Publication Date: 1977-08-01 PubMed ID: 69728PubMed Central: PMC515841DOI: 10.1128/JVI.23.2.384-393.1977Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers isolated a new retravirus from a squirrel monkey, which behaves differently in different cellular environments and has no relatedness to other known retraviruses.

Isolation and Identification of New Retravirus

  • The study characterized a retravirus, named Squirrel Monkey Retravirus (SMRV), which was isolated from a Squirrel Monkey, scientifically known as Saimiri sciureus.
  • The key characteristics that define SMRV include having an Mg2+-dependen reverse transcriptase and a buoyant density of 1.17 g/cm3 in sucrose and 1.21 g/cm3 in cesium chloride. These properties are similar to the mouse mammary tumor virus and the Mason-Pfizer monkey virus.
  • The distinguishing aspect of SMRV is its unique polypeptide pattern, which differentiates it from all other retraviruses. This fact was established by utilising sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Details of Polypeptide Patterns

  • In this study, four major polypeptides with molecular weights 40,000, 20,000, 14,000, and 8,000 were observed in the virus when it was propagated in human, mink, and canine cells.
  • The researchers identified the major viral glycoprotein in A204 human rhabdomyosarcoma cells as a protein weighing 73,000 daltons (gp73). The identification was accomplished through [3H]glucosamine labelling.
  • The researchers also found other proteins, but their presence was found to be dependent on the type of cells in which the virus propagated.

Antigenic Unrelatedness and Novelty

  • The researchers performed both species-specific and interspecies-specific assays to ascertain the antigenic relatedness between SMRV and other known retraviruses.
  • No antigenic relatedness was found between SMRV and other retraviruses, including recognizable ones like Mason-Pfizer monkey virus, mouse mammary tumor virus, baboon endogenous virus, woolly monkey virus, murine leukemia virus, endogenous feline type C virus, bovine leukemia virus, and equine infectious anemia virus.
  • This clear distinction in antigenic properties suggests that SMRV is indeed a new retravirus and the first of its kind to be isolated from a New World monkey.

Cite This Article

APA
Schochetman G, Fine D, Arthur L, Gilden R, Heberling R. (1977). Characterization of a retravirus isolated from squirrel monkeys. J Virol, 23(2), 384-393. https://doi.org/10.1128/JVI.23.2.384-393.1977

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 23
Issue: 2
Pages: 384-393

Researcher Affiliations

Schochetman, G
    Fine, D
      Arthur, L
        Gilden, R
          Heberling, R

            MeSH Terms

            • Animals
            • Antigens, Viral / analysis
            • Cell Line
            • Dogs
            • Epitopes
            • Haplorhini
            • Humans
            • Magnesium / pharmacology
            • Molecular Weight
            • Oncogenic Viruses / analysis
            • Oncogenic Viruses / enzymology
            • Oncogenic Viruses / growth & development
            • Peptides / analysis
            • RNA-Directed DNA Polymerase / metabolism
            • Saimiri
            • Viral Proteins / analysis
            • Viral Proteins / immunology
            • Virus Cultivation

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            Citations

            This article has been cited 9 times.
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