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Jaworska J, Tobolski D, Salem SE, Ropka-Molik K, Ząbek T, Szmatoła T, Kahler A, Wocławek-Potocka I, Piórkowska K, de Mestre AM.
(2026).
Comparative analysis of the transcriptome and methylome in distinct trophoblast populations at the time of embryo implantation in mares.
BMC Genomics.
https://doi.org/10.1186/s12864-026-12822-8
Department of Molecular Basis of Equine Reproduction, IAR & FR, PAS, Olsztyn, Poland. joanna.jaworska11@gmail.com.
Tobolski, Dawid
Department of Large Animal Diseases and Clinic, University of Life Sciences, Warsaw, Poland.
Salem, Shebl E
Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Department of Veterinary Clinical Sciences, Louisiana State University, Baton Rouge, Louisiana, USA.
Ropka-Molik, Katarzyna
Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice, 32-083, Poland.
Ząbek, Tomasz
Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice, 32-083, Poland.
Szmatoła, Tomasz
Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice, 32-083, Poland.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Agriculture, Krakow, Poland.
Kahler, Anne
Department of Comparative Biomedical Sciences, Royal Veterinary College, Hatfield, Hertfordshire, UK.
Wocławek-Potocka, Izabela
Department of Gamete Biology, IAR&FR, PAS, Olsztyn, Poland.
Piórkowska, Katarzyna
Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice, 32-083, Poland.
de Mestre, Amanda M
Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Department of Comparative Biomedical Sciences, Royal Veterinary College, Hatfield, Hertfordshire, UK.
Grant Funding
NCN 2020/36/C/NZ9/00192 / Narodowe Centrum Nauki
Conflict of Interest Statement
Declarations. Ethical approval and consent to participate: All animal procedures were conducted in accordance with the UK Animals (Scientific Procedures) Act 1986 (as revised) and the EU Directive 2010/63/EU for animal experiments. The study was approved by the Royal Veterinary College Ethics and Welfare Committee, specifically its Animal Welfare Ethical Review Body (AWERB) under UK Home Office project license PL70/8577. For the non-surgical uterine lavage procedure, mares were restrained in stocks and sedated with detomidine hydrochloride (Dormosedan, 10 mg/mL; Pfizer, UK) at a dose of 0.01 mg/kg body weight, administered intravenously. The mares were not euthanised, anaesthetised, or rendered unconscious; detomidine hydrochloride is a potent sedative and analgesic that induces a state of reduced awareness and provides pain relief. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
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