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Home / Equine Research Bank / Vet Sci / Comparison of Two Leptospira Type Strains of Serovar Grippot...
Veterinary sciences2022; 9(9); 464; doi: 10.3390/vetsci9090464

Comparison of Two Leptospira Type Strains of Serovar Grippotyphosa in Microscopic Agglutination Test (MAT) Diagnostics for the Detection of Infections with Leptospires in Horses, Dogs and Pigs.

Abstract: The MAT test is of great importance in the diagnosis of leptospiral infections. Based on various differences, the serovar Grippotyphosa has been divided into two types, Moskva V and Duyster. Differences or similarities of the two type strains in the context of leptospiral diagnostics have not yet been elucidated in more detail; therefore both strains were analysed in MAT diagnostics for the detection of leptospiral infections in pigs, dogs and horses. Serum samples from 2996 pigs, 55 dogs and 35 horses, as well as vitreous and/or aqueous fluid samples from these and 13 additional horses were analysed by MAT; available supplementary samples were tested for leptospires by PCR. In pigs, 92.6% of the samples with both strains received an identical titre result in the MAT test, whereas in dogs and horses only 53.0% and 43.6% had concordant results. Since infections with the serovar Grippotyphosa occur more frequently in dogs and horses overall, more differences were observed here. In the case of discrepant serological results, supplementary samples and PCR examinations were not able to add information on the true status. Further analyses of follow-up studies or at least serum pairs from dogs and horses infected with the serovar Grippotyphosa are necessary.
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Publication Date: 2022-08-29 PubMed ID: 36136680PubMed Central: PMC9503138DOI: 10.3390/vetsci9090464Google Scholar: Lookup
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Summary

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The research analyzes and compares the effectivity of two type strains, Moskva V and Duyster, of the serovar Grippotyphosa, in the Microscopic Agglutination Test (MAT) for diagnosing leptospiral infections in pigs, dogs, and horses.

Research Objectives

  • This study aims to examine the differences or similarities between two type strains, Moskva V and Duyster, of the serovar Grippotyphosa, in the context of leptospiral diagnostics.
  • It also strives to provide insight into the effectiveness of these strains when used in MAT diagnostics for detecting leptospiral infections in pigs, dogs, and horses.

Methodology

  • Samples were taken from a population of 2996 pigs, 55 dogs, and 35 horses.
  • Vitreous and/or aqueous fluid samples were also collected from these animal groups and 13 additional horses.
  • The samples were tested with the MAT; supplementary samples were further evaluated for leptospires using PCR.

Findings

  • In pigs, identical titre results were received in 92.6% of the samples with both strains in the MAT test.
  • However, in dogs and horses, only 53.0% and 43.6% had matching outcomes respectively.
  • As infections with the serovar Grippotyphosa are more common in dogs and horses, more significant differences were noticed in these groups.
  • On using supplementary samples and PCR examinations for cases with divergent serological results, no additional information on the true status could be derived.

Conclusion

  • The research concludes that additional evaluations regarding follow-up studies or at least serum pairs from dogs and horses infected with the serovar Grippotyphosa are needed for complete understanding.
  • Within the scope of this study, the two type strains have shown certain variances in their effectiveness during MAT diagnostics, especially in the detection of leptospiral infection in dogs and horses.

Cite This Article

APA
Strutzberg-Minder K, Ullerich A, Dohmann K, Boehmer J, Goris M. (2022). Comparison of Two Leptospira Type Strains of Serovar Grippotyphosa in Microscopic Agglutination Test (MAT) Diagnostics for the Detection of Infections with Leptospires in Horses, Dogs and Pigs. Vet Sci, 9(9), 464. https://doi.org/10.3390/vetsci9090464

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 9
Issue: 9
PII: 464

Researcher Affiliations

Strutzberg-Minder, Katrin
  • IVD Innovative Veterinary Diagnostics (IVD GmbH), 30926 Seelze, Germany.
Ullerich, Astrid
  • IVD Innovative Veterinary Diagnostics (IVD GmbH), 30926 Seelze, Germany.
Dohmann, Karen
  • IVD Innovative Veterinary Diagnostics (IVD GmbH), 30926 Seelze, Germany.
Boehmer, Jan
  • IVD Innovative Veterinary Diagnostics (IVD GmbH), 30926 Seelze, Germany.
Goris, Marga
  • OIE and National Collaborating Centre for Reference and Research on Leptospirosis, Department of Medical Microbiology and Infection Prevention, Amsterdam University Medical Centre, 1105 AZ Amsterdam, The Netherlands.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 2 times.
  1. Aguilar-Montes de Oca S, Montes-de-Oca-Jiménez R, Carlos Vázquez-Chagoyán J, Barbabosa-Pliego A, Eliana Rivadeneira-Barreiro P, C Zambrano-Rodríguez P. The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review. Vet Sci 2022 Oct 12;9(10).
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