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Home / Equine Research Bank / Scand J Clin Lab Invest / Concentration of sodium hyaluronate in serum.
Scandinavian journal of clinical and laboratory investigation1985; 45(6); 497-504; doi: 10.3109/00365518509155249

Concentration of sodium hyaluronate in serum.

Abstract: A radioassay for sodium hyaluronate using high-affinity binding protein from bovine cartilage has been modified for serum analysis. The accuracy of the method was checked by isotope dilution experiments and by recovery studies with exogenous hyaluronate. The between-assay standard deviation in the determination is 15-20%. The concentration of sodium hyaluronate in healthy adults (blood donors) is in the range of 10 to 100 micrograms/l with a mean value in the order of 30 to 40 micrograms/l. This is a lower concentration than previously reported. The same level was found in young people. Higher hyaluronate concentrations were noted in persons above 50 years of age. Analysis of plasma showed a slightly higher average hyaluronate level (5%) than in serum from the same persons. There were no notable sex differences. Analysis of serum and plasma from adult animals (rat, rabbit, dog, pig, goat, sheep, cow and horse) gave hyaluronate concentrations of the same order or higher than in human serum.
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Publication Date: 1985-10-01 PubMed ID: 3906849DOI: 10.3109/00365518509155249Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This paper presents a modified radioassay for measuring sodium hyaluronate concentration in serum, indicating a lower level in healthy adults and young people than previously reported, with higher levels in people over 50. No significant gender differences are noted, while animal serum revealed similar or higher hyaluronate concentrations.

Research Methodology

  • The research modified an existing radioassay methodology for measuring sodium hyaluronate, using a high-affinity binding protein from bovine cartilage, to allow for serum analysis.
  • The accuracy of this modified method was confirmed through isotope dilution experiments and recovery studies using external hyaluronate.
  • The standard deviation between each assay’s determination ranged from 15-20%, indicating a reasonable level of consistency in the results.

Hyaluronate Concentrations in Humans

  • The study found the concentration of sodium hyaluronate in healthy adults and younger people falls between 10 to 100 micrograms/l, with an average around 30 to 40 micrograms/l.
  • Previous studies had shown higher concentrations, making this research noteworthy for its lower findings.
  • Persons over the age of 50 displayed higher hyaluronate concentrations, highlighting a probable correlation between age and hyaluronate levels.
  • Analysis of plasma and serum from the same individuals revealed a slightly higher average concentration of hyaluronate in plasma, by approximately 5%.
  • No appreciable differences in hyaluronate concentrations were found between genders, suggesting sex does not substantially impact hyaluronate levels.

Hyaluronate Concentrations in Animals

  • The study analysed serum and plasma from several adult animals: rats, rabbits, dogs, pigs, goats, sheep, cows, and horses.
  • The measured hyaluronate concentrations for these animals were generally the same or higher than their human counterparts, suggesting species variation in sodium hyaluronate levels.

Cite This Article

APA
Engström-Laurent A, Laurent UB, Lilja K, Laurent TC. (1985). Concentration of sodium hyaluronate in serum. Scand J Clin Lab Invest, 45(6), 497-504. https://doi.org/10.3109/00365518509155249

Publication

Scandinavian journal of clinical and laboratory investigation
ISSN: 0036-5513
NlmUniqueID: 0404375
Country: England
Language: English
Volume: 45
Issue: 6
Pages: 497-504

Researcher Affiliations

Engström-Laurent, A
    Laurent, U B
      Lilja, K
        Laurent, T C

          MeSH Terms

          • Adolescent
          • Adult
          • Age Factors
          • Aged
          • Animals
          • Cattle
          • Child
          • Child, Preschool
          • Dogs
          • Female
          • Goats
          • Horses
          • Humans
          • Hyaluronic Acid / blood
          • Male
          • Middle Aged
          • Rabbits
          • Radioisotope Dilution Technique
          • Radioligand Assay
          • Rats
          • Sex Factors
          • Sheep
          • Species Specificity
          • Swine

          Citations

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