Continuous infusion of gentamicin into the tarsocrural joint of horses.
Abstract: To develop a method for continuous infusion of gentamicin into the tarsocrural joint of horses, to determine pharmacokinetics of gentamicin in synovial fluid of the tarsocrural joint during continuous infusion, and to evaluate effects of continuous infusion of gentamicin on characteristics of the synovial fluid. Methods: 12 healthy adult horses. Methods: An infusion catheter consisting of flow control tubing connected to a balloon infuser was used. Gentamicin solution (100 mg/ml) was infused in the right tarsocrural joint and balanced electrolyte solution was infused in the left tarsocrural joint for 5 days. Synovial fluid and serum gentamicin concentrations were measured by use of a fluorescence polarization immunoassay. Results: 17 of the 24 (71%) infusion catheters initially placed functioned without complications for the entire 5-day infusion period. Median gentamicin concentration in synovial fluid from treated joints during the 5-day infusion period ranged from 2875 to 982 microg/ml. Median serum gentamicin concentration during this period ranged from 2.31 to 2.59 microg/ml. Mean (+/- SD) elimination half-life and total clearance of gentamicin from the synovial fluid were 6.25+/-1.01 hours and 1.52+/-0.96 ml/min, respectively. Conclusions: An infusion catheter can be used for continuous infusion of gentamicin into the tarsocrural joints of horses for up to 5 days. At a gentamicin dosage of 0.17+/-0.02 mg/kg/h, continuous intra-articular infusion results in synovial fluid gentamicin concentrations greater than 100 times the minimal inhibitory concentration reported for common equine pathogens.
Publication Date: 2000-04-20 PubMed ID: 10772105DOI: 10.2460/ajvr.2000.61.407Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study documents the development and testing of a technique for the continuous supply of the antibiotic gentamicin into the tarsocrural joint (ankle joint) of horses. This process can potentially treat joint infections by delivering targeted, high concentrations of the antibiotic.
Methodology
- The researchers used 12 healthy adult horses for the study.
- They developed an infusion catheter specifically for the experiment – with flow control tubing attached to a balloon infuser.
- The right tarsocrural joint of the horses was infused with a gentamicin solution (100 mg/ml) for 5 days, while the left joint received a balanced electrolyte solution.
Measurement & Results
- During the experiment, gentamicin concentrations in the synovial fluid (lubricating fluid in the joint) and serum (blood) were monitored using a fluorescence polarization immunoassay (a biochemical technique to measure antibody interactions).
- Out of the 24 infusion catheters that were initially placed, 17 (or 71%) successfully served the full 5-day period of infusion without complications.
- Upon evaluation, the gentamicin concentrations in synovial fluid ranged between 2875 and 982 micrograms per milliliter.
- Serum gentamicin concentrations varied between 2.31 and 2.59 micrograms per milliliter.
- The researchers also found that the elimination half-life (time taken for the drug concentration to reduce by half) and total clearance of gentamicin from the synovial fluid were around 6.25 hours and 1.52 ml/min, respectively.
Conclusions
- The study concludes that an infusion catheter can be effectively used for up to 5 days to provide a consistent infusion of gentamicin into the horse’s tarsocrural joint.
- They also report that when administered at a dosage of approximately 0.17 milligrams per kilogram per hour, continuous infusion yields gentamicin concentrations in the synovial fluid that are over a hundred times the minimum inhibitory concentration required to treat common equine pathogens.
Cite This Article
APA
Lescun TB, Adams SB, Wu CC, Bill RP.
(2000).
Continuous infusion of gentamicin into the tarsocrural joint of horses.
Am J Vet Res, 61(4), 407-412.
https://doi.org/10.2460/ajvr.2000.61.407 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
MeSH Terms
- Animals
- Anti-Bacterial Agents / administration & dosage
- Anti-Bacterial Agents / blood
- Anti-Bacterial Agents / pharmacokinetics
- Catheterization
- Female
- Gentamicins / administration & dosage
- Gentamicins / blood
- Gentamicins / pharmacokinetics
- Horses / blood
- Horses / metabolism
- Joints
- Male
- Orthopedics / methods
- Orthopedics / veterinary
- Synovial Fluid / chemistry
- Tarsus, Animal
Citations
This article has been cited 1 times.- Yamaura K, Sather NA, Metlushko A, Nishimura H, Pavlović RZ, Hambright S, Ravuri SK, Philippon MJ, Stupp SI, Bahney CS, Huard J. Sustained-release losartan from peptide nanofibers promotes chondrogenesis. Front Bioeng Biotechnol 2023;11:1122456.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
