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The Journal of antimicrobial chemotherapy2014; 69(5); 1224-1230; doi: 10.1093/jac/dkt516

CTX-M-15-D-ST648 Escherichia coli from companion animals and horses: another pandemic clone combining multiresistance and extraintestinal virulence?

Abstract: To discern the relevance of ST648 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli as a putative new group of multiresistant and extraintestinal pathogenic strains in animals, its frequency, ESBL types, antimicrobial resistance patterns and virulence gene (VG) profiles should be determined and compared with ST131 strains from the same collection of strains. Methods: ESBL-producing E. coli isolates (n = 1152), consecutively sampled from predominantly dogs, cats and horses between 2008 and 2011, were assigned to a phylogenetic group by PCR. Partial multilocus sequence typing was performed for group D and B2 strains and strains presumed to be D-ST648 and B2-ST131 were fully typed. ESBL genes and extraintestinal pathogenic E. coli (ExPEC)-like VGs were characterized by PCR and sequence analysis and antimicrobial resistance was determined by broth dilution. Clonal analysis was done by PFGE. Results: Forty (3.5%) ESBL-producing E. coli were determined as D-ST648, whereas B2-ST131 isolates occurred less frequently (2.8%). Although the predominant ESBL type in both groups was CTX-M-15 (72.5% versus 46.9%), ST648 strains from companion animals and horses displayed a lower variety of ESBL types (CTX-M-1, -3, -14, -15 and -61 versus CTX-M-1,-2,-14,-15,-27 and -55 and SHV-12). In contrast to ST131 strains, a higher proportion of ST648 strains showed resistance to most non-β-lactam antibiotics. Overall, VGs were less abundant in ST648 strains, although some strains had VG profiles comparable to those of ST131 strains. ExPEC-associated serotype O1:H6 was predominant (46.8%) among the ST648 strains. Some PFGE clusters comprised ST648 isolates from pets, horses and wild birds and humans included from previous studies. Conclusions: Our findings demonstrate that certain subgroups of E. coli D-ST648-CTX-M may represent a novel genotype that combines multiresistance, extraintestinal virulence and zoonotic potential.
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Publication Date: 2014-01-06 PubMed ID: 24398338DOI: 10.1093/jac/dkt516Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the significance of a particular strain of Escherichia coli, ST648 extended-spectrum β-lactamase (ESBL)-producing, as a potential new group of multi-resistant and extra-intestinal pathogenic strains in animals. The research was carried out on samples obtained from predominantly dogs, cats, and horses between 2008 and 2011.

Methodology

  • 1152 ESBL-producing E. coli isolates were examined. The phylogenetic group was identified using PCR (polymerase chain reaction) methods.
  • Partial multilocus sequence typing was done on group D and B2 strains, and the ones assumed to be D-ST648 and B2-ST131 were fully typed.
  • ESBL genes and extraintestinal pathogenic E. coli (ExPEC)-like virulence genes (VGs) were characterized by PCR and sequence analysis. Antimicrobial resistance was determined by broth dilution.
  • Clonal analysis was done by PFGE (Pulsed Field Gel Electrophoresis).

Results

  • D-ST648 was found to be present in 3.5% of ESBL-producing E. coli, while B2-ST131 type was slightly less common (2.8%).
  • The CTX-M-15 was the predominant ESBL type in both groups, although ST648 strains had a lower variety of ESBL types.
  • ST648 strains showed higher resistance levels to most non-β-lactam antibiotics compared to ST131 strains. However, virulence genes were less abundant in ST648 strains. Some strains of ST648 shared similar VG profiles with ST131 strains.
  • The ExPEC-associated serotype O1:H6 was the most common among the ST648 strains. After performing PFGE, some clusters were found to include ST648 isolates from pets, horses, birds, and also humans from previous studies.

Conclusions

  • The findings demonstrate that some subgroups of E. coli D-ST648-CTX-M-15 may constitute a new genotype that combines multiresistance to antibiotics, extraintestinal virulence, and zoonotic (transferrable from animals to humans) potential.

Conclusively, the study uncovers the potential hazards of this E. coli strain, emphasizing the need for further research and appropriate preventive measures.

Cite This Article

APA
Ewers C, Bethe A, Stamm I, Grobbel M, Kopp PA, Guerra B, Stubbe M, Doi Y, Zong Z, Kola A, Schaufler K, Semmler T, Fruth A, Wieler LH, Guenther S. (2014). CTX-M-15-D-ST648 Escherichia coli from companion animals and horses: another pandemic clone combining multiresistance and extraintestinal virulence? J Antimicrob Chemother, 69(5), 1224-1230. https://doi.org/10.1093/jac/dkt516

Publication

The Journal of antimicrobial chemotherapy
ISSN: 1460-2091
NlmUniqueID: 7513617
Country: England
Language: English
Volume: 69
Issue: 5
Pages: 1224-1230

Researcher Affiliations

Ewers, Christa
  • Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-Universität Giessen, Frankfurter Str. 85-89, 35392 Giessen, Germany.
Bethe, Astrid
    Stamm, Ivonne
      Grobbel, Mirjam
        Kopp, Peter A
          Guerra, Beatriz
            Stubbe, Michael
              Doi, Yohei
                Zong, Zhiyong
                  Kola, Axel
                    Schaufler, Katharina
                      Semmler, Torsten
                        Fruth, Angelika
                          Wieler, Lothar H
                            Guenther, Sebastian

                              MeSH Terms

                              • Animals
                              • Anti-Bacterial Agents / pharmacology
                              • Cats
                              • Dogs
                              • Escherichia coli / classification
                              • Escherichia coli / enzymology
                              • Escherichia coli / genetics
                              • Escherichia coli / isolation & purification
                              • Escherichia coli Infections / epidemiology
                              • Escherichia coli Infections / microbiology
                              • Escherichia coli Infections / veterinary
                              • Genotype
                              • Horses
                              • Microbial Sensitivity Tests
                              • Multilocus Sequence Typing
                              • Pets
                              • Phenotype
                              • Polymerase Chain Reaction
                              • Virulence Factors / genetics
                              • beta-Lactamases / genetics

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