Cytolytic activity of liposomes containing stearylamine.
Abstract: In order to develop the cytotoxic liposome, the cytolytic effect of polycationic liposome was examined. Upon incubation of the stearylamine-containing liposome (stearylamine-liposome) with rabbit erythrocyte, a significant extent of hemolysis was observed. Hemolytic activity of the liposome depends on the amount of stearylamine in the liposome membrane. The plots of the initial rate of hemolysis versus the concentration of stearylamine-liposome showed a sigmoidal curve, suggesting that stearylamine-liposomes act cooperatively on the erythrocyte membrane. Hemolytic activity of stearylamine-liposome was markedly influenced by the composition of hydrocarbon chains of the phospholipids in the liposome membrane, suggesting that the membrane fluidity of stearylamine-liposome is important to evoke the hemolysis. Since the liposomes containing acidic phospholipids inhibited markedly the stearylamine-liposome-caused hemolysis, it is likely that the primary target of stearylamine-liposome is the negatively charged component(s) such as acidic phospholipids on the erythrocyte membrane. Furthermore, stearylamine-liposome induced the release of the intravesicular contents from the liposome made of acidic phospholipids but not from the liposome made of phosphatidylcholine only. These results suggest that stearylamine-liposome interacted with the negative charges of the erythrocyte membrane and eventually damaged the cell. Erythrocytes from rabbit, horse and guinea pig are highly susceptible to stearylamine-liposome but those from man, sheep, cow and chicken are less so.
Publication Date: 1986-01-16 PubMed ID: 3942721DOI: 10.1016/0005-2736(86)90068-4Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers are finding out more about the cytolysis effects of liposomes (tiny bubble-like structures used in drug delivery) containing stearylamine, an ingredient that shows promise in developing cytotoxic liposomes for cancer treatment.
Objective
- The aim of the study is to analyze and understand the cytolytic effect of liposomes containing stearylamine, which could aid in the creation of more effective cytotoxic liposomes for cancer treatment. One of the main goals was to examine the hemolytic (red blood cell destruction) activity of these liposomes.
Methodology
- The researchers incubated stearylamine-liposomes with rabbit erythrocytes (red blood cells). The amount of hemolysis observed was measured and analyzed.
- To better understand the influence of stearylamine, the research team scrutinized the relationship between its concentration in the liposome membrane and its hemolytic activity.
Findings
- An increase in the concentration of stearylamine-liposome led to an increased initial rate of hemolysis, depicted in a sigmoidal curve. It implied that these liposomes act cooperatively on the erythrocyte membrane to instigate the hemolysis.
- The composition of hydrocarbon chains in the liposome membrane plays a significant role in the hemolytic activity of stearylamine-liposome. This implies that membrane fluidity is a key factor in provoking hemolysis.
- Liposomes containing acidic phospholipids notably impeded hemolysis caused by stearylamine-liposome, suggesting that stearylamine-liposomes primarily target and act upon negatively charged components of the erythrocyte membrane such as acidic phospholipids.
- Stearylamine-liposomes could induce release of contents from liposomes made of acidic phospholipids but not from those made solely of phosphatidylcholine. This further validates the idea of negative charges on erythrocyte membrane being the main target.
Conclusion
- The study shows that the action of stearylamine-liposome could cause damage to erythrocytes through their interaction and eventually destroying the cells.
- The susceptibility to stearylamine-liposome varied across species, with erythrocytes from rabbits, horses, and guinea pigs showing high susceptibility, while those from humans, sheep, cows, and chickens exhibited lesser susceptibility.
Cite This Article
APA
Yoshihara E, Nakae T.
(1986).
Cytolytic activity of liposomes containing stearylamine.
Biochim Biophys Acta, 854(1), 93-101.
https://doi.org/10.1016/0005-2736(86)90068-4 Publication
Researcher Affiliations
MeSH Terms
- Amines
- Animals
- Cattle
- Chickens
- Erythrocytes
- Fatty Acids / physiology
- Fluoresceins
- Guinea Pigs
- Hemolysis
- Horses
- Humans
- Hydrogen-Ion Concentration
- Liposomes
- Membrane Fluidity
- Phosphatidylserines / physiology
- Phospholipids / physiology
- Rabbits
- Sheep
- Species Specificity
Citations
This article has been cited 12 times.- Sriwidodo, Umar AK, Wathoni N, Zothantluanga JH, Das S, Luckanagul JA. Liposome-polymer complex for drug delivery system and vaccine stabilization. Heliyon 2022 Feb;8(2):e08934.
- Suyamud C, Phetdee C, Jaimalai T, Prangkio P. Silk Fibroin-Coated Liposomes as Biomimetic Nanocarrier for Long-Term Release Delivery System in Cancer Therapy. Molecules 2021 Aug 15;26(16).
- Souto EB, Dias-Ferreira J, Craveiro SA, Severino P, Sanchez-Lopez E, Garcia ML, Silva AM, Souto SB, Mahant S. Therapeutic Interventions for Countering Leishmaniasis and Chagas's Disease: From Traditional Sources to Nanotechnological Systems. Pathogens 2019 Aug 1;8(3).
- De M, Ghosh S, Sen T, Shadab M, Banerjee I, Basu S, Ali N. A Novel Therapeutic Strategy for Cancer Using Phosphatidylserine Targeting Stearylamine-Bearing Cationic Liposomes. Mol Ther Nucleic Acids 2018 Mar 2;10:9-27.
- Rajendran V, Rohra S, Raza M, Hasan GM, Dutt S, Ghosh PC. Stearylamine Liposomal Delivery of Monensin in Combination with Free Artemisinin Eliminates Blood Stages of Plasmodium falciparum in Culture and P. berghei Infection in Murine Malaria. Antimicrob Agents Chemother 2015 Dec 14;60(3):1304-18.
- Mattheolabakis G, Nie T, Constantinides PP, Rigas B. Sterically stabilized liposomes incorporating the novel anticancer agent phospho-ibuprofen (MDC-917): preparation, characterization, and in vitro/in vivo evaluation. Pharm Res 2012 Jun;29(6):1435-43.
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- Parthasarathy R, Sacks PG, Harris D, Brock H, Mehta K. Interaction of liposome-associated all-trans-retinoic acid with squamous carcinoma cells. Cancer Chemother Pharmacol 1994;34(6):527-34.
- Tachibana H, Yoshihara E, Kaneda Y, Nakae T. In vitro lysis of the bloodstream forms of Trypanosoma brucei gambiense by stearylamine-bearing liposomes. Antimicrob Agents Chemother 1988 Jul;32(7):966-70.
- Huang R, Zhang F, Wang X, Yang F, Ma C. Proteomic Profiling in Pediococcus pentosaceus SF11 Exposed to Condensed Tannins from Sainfoin. ACS Omega 2024 Oct 8;9(40):41148-41156.
- Hussain A, Altamimi MA, Alneef YS. HSPiP and QbD oriented optimized stearylamine-elastic liposomes for topical delivery of ketoconazole to treat deep seated fungal infections: In vitro and ex vivo evaluations. Int J Pharm X 2024 Dec;8:100279.
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