Cytotoxic Natural Products Isolated from Cryptogramma crispa (L.) R. Br.
Abstract: Parsley fern, , is a common fern in arctic-alpine regions, and even though this species has been known since ancient times and has been presumed to cause the poisoning of horses, its natural products have not previously been investigated. Here, we characterise 15 natural products isolated from the aerial parts of , including the previously undescribed compound 3-malonyl pteroside D. The structure determinations were based on several advanced 1D and 2D NMR spectroscopic techniques, Circular Dichroism spectroscopy and high-resolution mass spectrometry. The pteroside derivatives exhibited selective moderate cytotoxic activity against the acute myeloid leukaemia MOLM13 cell line and no cytotoxicity against the normal heart and kidney cell lines, suggesting that their potential anticancer effect should be further investigated.
Publication Date: 2023-11-23 PubMed ID: 38067454PubMed Central: PMC10708030DOI: 10.3390/molecules28237723Google Scholar: Lookup
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Summary
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The research article investigates the natural products derived from Parsley fern, commonly found in arctic-alpine regions. The study identifies and characterizes 15 natural compounds, including a previously unknown compound and investigates their potential anticancer effects by testing their impact on leukaemia and normal cell lines.
Introduction and Research Methodology
- The research article is centered around the study of Cryptogramma crispa, or Parsley fern. This is a common plant found in arctic-alpine regions and is known to cause horse poisoning.
- Prior to this research, there had been no investigation of the natural products derived from this plant.
- What this research does, for the first time, is discover and characterize 15 natural products which originate from the aerial parts of the Parsley fern.
- The discovery of these compounds included the first ever identification of the compound 3-malonyl pteroside D.
- The primary techniques used to determine the structure of these compounds included advanced 1D and 2D NMR spectroscopic techniques, Circular Dichroism spectroscopy and high-resolution mass spectrometry.
Investigation of Cytotoxic Activity
- The isolated compounds were tested for their cytotoxic activity, essentially, their ability to kill cells.
- More specifically, their effectiveness was tested against the acute myeloid leukaemia MOLM13 cell line. These are a type of cancer cell derived from leukemia patients, frequently used in medical research.
- The results revealed that the isolated compounds show moderate cytotoxic activity against these malignant cells.
- However, it is noteworthy that these compounds did not exhibit cytotoxicity against normal heart and kidney cell lines.
Implications and Conclusion
- The selective cytotoxicity of these compounds suggests potential anticancer applications.
- The results show promise in the pursuit of new treatments for acute myeloid leukaemia.
- The authors of the paper argue that this potential anticancer effect warrants further investigation and research.
- Given the limitations of the study, this research represents an initial step towards understanding the potential medical applications of Compounds derived from Cryptogramma crispa.
Cite This Article
APA
Diaz AEC, Herfindal L, Andersen HL, Fossen T.
(2023).
Cytotoxic Natural Products Isolated from Cryptogramma crispa (L.) R. Br.
Molecules, 28(23), 7723.
https://doi.org/10.3390/molecules28237723 Publication
Researcher Affiliations
- Department of Chemistry and Centre for Pharmacy, University of Bergen, N-5007 Bergen, Norway.
- Department of Clinical Science and Centre for Pharmacy, University of Bergen, N-5009 Bergen, Norway.
- University Gardens, University of Bergen, Allégt. 41, N-5007 Bergen, Norway.
- Department of Chemistry and Centre for Pharmacy, University of Bergen, N-5007 Bergen, Norway.
Grant Funding
- 180007 / Norwegian Society for Children's Cancer
- 190004 / Norwegian Society for Children's Cancer
- 226244/F50 / The Research Council of Norway
Conflict of Interest Statement
The authors declare no conflict of interest.
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