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BMC research notes2023; 16(1); 267; doi: 10.1186/s13104-023-06562-1

Decoding the transcriptomic expression and genomic methylation patterns in the tendon proper and its peritenon region in the aging horse.

Abstract: Equine tendinopathies are challenging because of the poor healing capacity of tendons commonly resulting in high re-injury rates. Within the tendon, different regions - tendon proper (TP) and peritenon (PERI) - contribute to the tendon matrix in differing capacities during injury and aging. Aged tendons have decreased repair potential; the underlying transcriptional and epigenetic changes that occur in the TP and PERI regions are not well understood. The objective of this study was to assess TP and PERI regional differences in adolescent, midlife, and geriatric horses using RNA sequencing and DNA methylation techniques. Results: Differences existed between TP and PERI regions of equine superficial digital flexor tendons by age as evidenced by RNASeq and DNA methylation. Cluster analysis indicated that regional distinctions existed regardless of age. Genes such as DCN, COMP, FN1, and LOX maintained elevated TP expression while genes such as GSN and AHNAK were abundant in PERI. Increased gene activity was present in adolescent and geriatric populations but decreased during midlife. Regional differences in DNA methylation were also noted. Notably, when evaluating all ages of TP against PERI, five genes (HAND2, CHD9, RASL11B, ADGRD1, and COL14A1) had regions of differential methylation as well as differential gene expression.
© 2023. BioMed Central Ltd., part of Springer Nature.
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Publication Date: 2023-10-11 PubMed ID: 37821884PubMed Central: PMC10566085DOI: 10.1186/s13104-023-06562-1Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is focused on understanding the changes in transcriptomic expression and genomic methylation in different parts of the tendon in horses as they age. This is achieved through the comparison of these patterns in the two regions of tendons known as the tendon proper and peritenon in horses during different stages of life – adolescence, middle age, and old age.

Study Overview and Objective

  • The primary goal of this research was to investigate the differences in the regions of the tendon proper (TP) and peritenon (PERI) in horses during different life stages. This was achieved by utilizing RNA sequencing and DNA methylation techniques.
  • The researchers wanted to understand the varying contributions of these two regions to the tendon matrix during aging and injury. The differences in transcriptional and epigenetic changes in these areas are not well understood, especially when considering the decreased repair potential of aged tendons.

Results of the Research

  • The research results revealed significant differences in the TP and PERI regions of equine superficial digital flexor tendons based on age. This was determined using the RNASeq results and DNA methylation patterns.
  • The cluster analysis also revealed that there are regional differences that exist beyond the normal impacts of age.
  • For instance, some genes like DCN, COMP, FN1, and LOX were found to have elevated expression in TP while genes like GSN and AHNAK were more abundant in PERI.

Implications of the Findings

  • The results showed that there are regional variations in gene activity in adolescent and geriatric populations, with a dip in activity during midlife.
  • The DNA methylation patterns also showed regional differences.
  • Significantly, the study discovered that there are five genes (HAND2, CHD9, RASL11B, ADGRD1, and COL14A1) which show differences in both methylation and gene expression when comparing all ages of TP against PERI. This discovery could have implications for our understanding of tendon degradation and problems in aging horses.”

Conclusion

  • Overall, this research provides new insights into the genomic and epigenetic changes in TP and PERI regions of equine tendons across different age groups, and opens up avenues for further investigation into the impact of these changes on the healing capacity and injury rates of aged horses’ tendons.

Cite This Article

APA
Pechanec MY, Mienaltowski MJ. (2023). Decoding the transcriptomic expression and genomic methylation patterns in the tendon proper and its peritenon region in the aging horse. BMC Res Notes, 16(1), 267. https://doi.org/10.1186/s13104-023-06562-1

Publication

BMC research notes
ISSN: 1756-0500
NlmUniqueID: 101462768
Country: England
Language: English
Volume: 16
Issue: 1
Pages: 267
PII: 267

Researcher Affiliations

Pechanec, Monica Y
  • Department of Animal Science, University of California Davis, 2251 Meyer Hall, One Shields Ave, Davis, CA, 95616, USA.
Mienaltowski, Michael J
  • Department of Animal Science, University of California Davis, 2251 Meyer Hall, One Shields Ave, Davis, CA, 95616, USA. mjmienaltowski@ucdavis.edu.

MeSH Terms

  • Horses / genetics
  • Animals
  • Transcriptome
  • Methylation
  • Tendons / metabolism
  • Aging / genetics
  • Genomics
  • Horse Diseases

Grant Funding

  • D17EQ-818 / Morris Animal Foundation, United States

Conflict of Interest Statement

The authors declare no competing interests.

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