Demonstration isolation and identification of culturable microfungi and bacteria in horse hair and dandruff. Immunochemical comparison with allergic components.
Abstract: Horse hiar and dandruff have been investigated for their content of microfungi and bacteria. Inoculation and incubation on V-8 agar containing penicillin and streptomycin, with subsequent colony counting and identification, revealed more than nine and five different genera of microfungi and bacteria respectively, in horse hair and dandruff. Isolation and cultivation of the quantitatively dominating species, and preparation of an extract of these were performed, followed by immunochemical comparison with extract of the horse hair and dandruff using crossed-line immuno-electrophoresis. As no immunochemical identity was demonstrated it was concluded that the identified microorganisms might serve as a guideline to suspected sensitizing substances when patients with a typical case history of horse allergy do not react to extracts of horse hair and dandruff.
Publication Date: 1978-04-01 PubMed ID: 356659DOI: 10.1111/j.1398-9995.1978.tb01514.xGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research article is about investigating the types of microfungi and bacteria found in horse hair and dandruff. The researchers then compare these findings to the substances that typically cause allergic reactions.
Research Overview
The main focus of the study was to explore the content of microfungi and bacteria in horse hair and dandruff. This was achieved using the method of inoculation and incubation on V-8 agar containing penicillin and streptomycin, procedures commonly used in microbiology to grow and isolate bacteria and fungi. This step was followed by counting and identifying colonies of these organisms.
- The research found more than nine different genera of microfungi and over five different bacteria genera in horse hair and dandruff.
- The most dominant species were isolated and cultivated further for deeper analysis.
Immunochemical Comparison
An important part of the research was the comparison of microorganisms with potential allergens.
- An extract was prepared from the isolated and cultivated organisms.
- This extract was compared with the extract from horse hair and dandruff using crossed-line immuno-electrophoresis, a technique used to study the antigen-antibody reaction.
- This step did not demonstrate any immunochemical identity, meaning that the substances responsible for allergic reactions in the horse hair and dandruff were not the same as these microorganisms.
Significance of the Research
The results from the study provide valuable insights for medical investigations, especially for patients with potential allergies related to horses.
- If patients with a history of horse allergy do not react to horse hair and dandruff extracts, these identified microorganisms could provide a guide to what might be causing the suspected sensitizing substances.
- This research therefore has significant implications for diagnosis and treatment of horse-related allergies.
Cite This Article
APA
Gravesen S, Løwenstein H, Weeke B.
(1978).
Demonstration isolation and identification of culturable microfungi and bacteria in horse hair and dandruff. Immunochemical comparison with allergic components.
Allergy, 33(2), 89-92.
https://doi.org/10.1111/j.1398-9995.1978.tb01514.x Publication
Researcher Affiliations
MeSH Terms
- Allergens / isolation & purification
- Animals
- Bacteria / isolation & purification
- Fungi / isolation & purification
- Hair / microbiology
- Horses
- Immunoelectrophoresis, Two-Dimensional
- Microbiological Techniques
- Scalp Dermatoses / microbiology
Citations
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