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Home / Equine Research Bank / Vet Res Commun / Demonstration of heterogeneous genotypes of Taylorella equig...
Veterinary research communications2001; 25(7); 565-575; doi: 10.1023/a:1017925216350

Demonstration of heterogeneous genotypes of Taylorella equigenitalis isolated from horses in six European countries by pulsed-field gel electrophoresis.

Abstract: Forty-six isolates of Taylorella equigenitalis were analysed by pulsed-field gel electrophoresis (PFGE) after separate digestion of the genomic DNA with ApaI and with NotI. The isolates had been obtained from horses in six European countries and were classified into 18 genotypes. In Belgium, 2 genotypes were detected in 2 isolates, in England 9 among 15, in Finland 2 in 2, in France 2 among 10, in Sweden 3 among 5, and in Switzerland 3 among 12. Two English isolates and 4 French isolates gave identical PFGE profiles to those of Kentucky 188 from the United States. A common genotype was found in 5 isolates from Belgium and England and also in 10 isolates from France and Switzerland. The analysis of genomic DNA from 12 isolates of T equigenitalis obtained from male horses in France, Sweden and Switzerland gave no evidence of a sex-related difference in the genomic DNA. Genomic DNA from 11 streptomycin (STM)-susceptible isolates obtained in Sweden and Switzerland were classified into four genotypes by PFGE. Each of the six genotypes determined among the 17 isolates from these two countries had single phenotypes for resistance or susceptibility to STM.
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Publication Date: 2001-10-05 PubMed ID: 11583379DOI: 10.1023/a:1017925216350Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the genetic diversity of Taylorella equigenitalis isolates from horses in six European countries. It found 18 genotypes among the 46 isolates analyzed, with no evidence of sex-related differences in their genomic DNA.

Methodology of the Study

  • The researchers analyzed the genomic DNA of 46 isolates of Taylorella equigenitalis from horses in Belgium, England, Finland, France, Sweden, and Switzerland using pulsed-field gel electrophoresis (PFGE).
  • PFGE was conducted after separate digestion of the genomic DNA using restriction enzymes ApaI and NotI.

Results Found in the Study

  • In total, 18 different genotypes were identified among the isolates studied.
  • In individual countries, there was diverse prevalence: Belgium had 2 genotypes among 2 isolates, England had 9 amongst 15, Finland had 2 in 2, France had 2 within 10, Sweden had 3 in 5, and Switzerland had 3 in 12.
  • The researchers also noticed similarities between some European isolates and the U.S. Kentucky 188 isolate. Two English and four French isolates showed identical PFGE profiles to Kentucky 188.
  • A common genotype was found in isolates from both Belgium and England as well as France and Switzerland.

Additional Observations

  • The study found no evidence of sex-related differences in the genomic DNA of the T. equigenitalis isolates from male horses from France, Sweden, and Switzerland.
  • Genomic DNA from streptomycin (an antibiotic)-susceptible isolates obtained in Sweden and Switzerland was classified into four genotypes by PFGE. The six genotypes from the 17 isolates from these two countries each had single phenotypes for either resistance or susceptibility to streptomycin. This could suggest a link between particular genotypes and antibiotic susceptibility, which could have implications for treatment strategies.

Cite This Article

APA
Kagawa S, Klein F, Corboz L, Moore JE, Murayama O, Matsuda M. (2001). Demonstration of heterogeneous genotypes of Taylorella equigenitalis isolated from horses in six European countries by pulsed-field gel electrophoresis. Vet Res Commun, 25(7), 565-575. https://doi.org/10.1023/a:1017925216350

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 25
Issue: 7
Pages: 565-575

Researcher Affiliations

Kagawa, S
  • Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
Klein, F
    Corboz, L
      Moore, J E
        Murayama, O
          Matsuda, M

            MeSH Terms

            • Animals
            • Drug Resistance, Bacterial
            • Electrophoresis, Gel, Pulsed-Field / veterinary
            • Europe
            • Female
            • Genotype
            • Gram-Negative Bacterial Infections / microbiology
            • Gram-Negative Bacterial Infections / veterinary
            • Horse Diseases / microbiology
            • Horses
            • Male
            • Microbial Sensitivity Tests / veterinary
            • Streptomycin / pharmacology
            • Taylorella equigenitalis / classification
            • Taylorella equigenitalis / drug effects
            • Taylorella equigenitalis / genetics

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            Citations

            This article has been cited 3 times.
            1. Aalsburg AM, Erdman MM. Pulsed-field gel electrophoresis genotyping of Taylorella equigenitalis isolates collected in the United States from 1978 to 2010.. J Clin Microbiol 2011 Mar;49(3):829-33.
              doi: 10.1128/JCM.00956-10pubmed: 21191049google scholar: lookup
            2. Tazumi A, Sekizuka T, Moore JE, Millar BC, Taneike I, Matsuda M. Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis.. Folia Microbiol (Praha) 2008;53(6):486-92.
              doi: 10.1007/s12223-008-0076-0pubmed: 19381472google scholar: lookup
            3. Kagawa S, Nagano Y, Tazumi A, Murayama O, Millar BC, Moore JE, Matsuda M. Nucleotide sequencing and analysis of 16S rDNA and 16S-23S rDNA internal spacer region (ISR) of Taylorella equigenitalis, as an important pathogen for contagious equine metritis (CEM).. Vet Res Commun 2006 May;30(4):343-55.
              doi: 10.1007/s11259-006-3304-6pubmed: 16502103google scholar: lookup
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