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Home / Equine Research Bank / Vet Microbiol / Detection of neuropathogenic strains of Equid Herpesvirus 1 ...
Veterinary microbiology2010; 147(1-2); 176-180; doi: 10.1016/j.vetmic.2010.06.014

Detection of neuropathogenic strains of Equid Herpesvirus 1 (EHV-1) associated with abortions in Germany.

Abstract: A single nucleotide polymorphism within EHV-1 gene ORF 30, which encodes for the viral DNA polymerase, allows the differentiation of the neuropathogenic (G2254) from non-neuropathogenic genotype (A2254). The aim of our study was to investigate the distribution of the neuropathogenic and non-neuropathogenic genotype of EHV-1 isolates associated with abortions in Germany. To determine the nucleotide sequence at the polymorphic site the amplification product of ORF 30 gene specific nested PCR was digested with restriction enzyme SalI and sequenced. Thirty-two EHV-1 isolates from six abortion outbreaks and 34 archived isolates from individual cases were obtained between 1987 and 2009 from stud farms in different regions of Germany. 89.4% (59/66) of the EHV-1 abortion isolates was of non-neuropathogenic genotype (N752/A2254) and 10.6% (7/66) contained the neuropathogenic marker (D752/G2254). Two out of seven EHV-1 abortion isolates with the mutation (G2254) came from the same outbreak and were derived from mares with neurological signs. Interestingly, the semen of a stallion from the same stud was tested positive for the neuropathogenic genotype (G2254) too. The other five EHV-1 strains came from individual abortion cases with no neurological signs. In addition to the A2254 to G2254 substitution, two EHV-1 reference strains (Ab4 and RacH) and one field isolate from an individual abortion case showed an exchange of adenine to cytosine at position 2258. In sum, we confirmed coherence between the occurrence of abortions and the non-neuropathogenic EHV-1 (A2254), but 10.6% of the abortion strains carried the mutation (G2254). The relevance of this finding as well as the role of the additional mutation and of stallions as carriers should be further investigated.
Copyright © 2010 Elsevier B.V. All rights reserved.
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Publication Date: 2010-06-22 PubMed ID: 20619972DOI: 10.1016/j.vetmic.2010.06.014Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the presence and distribution of two strains of Equid Herpesvirus 1 (EHV-1) that are associated with horse abortions in Germany. The study found that the majority of cases were caused by the non-neuropathogenic strain, but a significant portion was attributed to the neuropathogenic strain.

Methodology

  • The researchers differentiated between the neuropathogenic (G2254) and non-neuropathogenic (A2254) strains of EHV-1 through a single nucleotide polymorphism within EHV-1 gene ORF 30.
  • A specific nested PCR process was used to amplify the ORF 30 gene and the resulting product was sequenced using the restriction enzyme SalI to determine the nucleotide sequence at the polymorphic site.
  • They then analyzed 32 EHV-1 samples from six abortion outbreaks and 34 archived samples from individual cases which had occurred from 1987 to 2009 in various German stud farms.

Results

  • The analysis found that 89.4% (59 out of 66) of the EHV-1 abortion samples belonged to the non-neuropathogenic type.
  • The remaining 10.6% (7 out of 66) contained the neuropathogenic marker. Two of these seven samples were from the same outbreak and had come from mares showing neurological signs.
  • Interestingly, stallion semen from the same stud also tested positive for the neuropathogenic strain, raising the question about the role stallions could be playing in spreading this strain.
  • The remaining five samples came from individual cases not associated with neurological signs.

Additional Findings

  • The study also found that two EHV-1 reference strains (Ab4 and RacH) and one field isolate from an individual abortion case showed a mutation in the form of an exchange of adenine to cytosine at position 2258 along with the A2254 to G2254 substitution.

Conclusions

  • Though the study found a significant correlation between the occurrence of abortions and the non-neuropathogenic EHV-1 strain, there was also identified presence of the neuropathogenic strain in 10.6% of the abortion cases.
  • The relevance and implications of these findings, along with the additional mutations found and the potential role of stallions as carriers of the virus, remain to be further investigated.

Cite This Article

APA
Fritsche AK, Borchers K. (2010). Detection of neuropathogenic strains of Equid Herpesvirus 1 (EHV-1) associated with abortions in Germany. Vet Microbiol, 147(1-2), 176-180. https://doi.org/10.1016/j.vetmic.2010.06.014

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 147
Issue: 1-2
Pages: 176-180

Researcher Affiliations

Fritsche, A-K
  • Institute of Virology, Faculty of Veterinary Medicine, Free University of Berlin, Philippstr. 13, 10115 Berlin, Germany.
Borchers, K

    MeSH Terms

    • Abortion, Veterinary / virology
    • Animals
    • Female
    • Genotype
    • Germany
    • Herpesviridae Infections / veterinary
    • Herpesviridae Infections / virology
    • Herpesvirus 1, Equid / genetics
    • Herpesvirus 1, Equid / isolation & purification
    • Herpesvirus 1, Equid / pathogenicity
    • Horse Diseases / virology
    • Horses
    • Male
    • Pregnancy
    • Virulence Factors / genetics

    Citations

    This article has been cited 20 times.
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