Development of a peptide ELISA for discrimination between serological responses to equine herpesvirus type 1 and 4.
Abstract: A peptide-based enzyme-linked immunosorbent assay (ELISA) for discrimination between serological responses to equine herpesvirus type 1 (EHV-1) and 4 (EHV-4) was developed. Three and four peptides for EHV-1 and EHV-4, respectively, were designed and studied initially in the ELISA using sera from foals infected experimentally. The most promising peptide pair, derived from EHV-1 glycoprotein E and EHV-4 glycoprotein G, was evaluated further using acute and convalescent sera from horses infected experimentally and naturally as well as a panel of horse field sera. Ten pre- and post-vaccination serum pairs were similarly tested in the type-specific ELISA. The peptide ELISA was able to identify horses which had been infected with EHV-1 or EHV-4 as derived from the results using acute and convalescent sera collected from natural outbreaks. When applied to a set of field samples, the assay proved robust with respect to determining the EHV-1 and EHV-4 antibody status. Also, the peptide ELISA was able to detect type-specific seroconversion for EHV-1 in vaccinated animals. With further validation, the EHV-1/EHV-4 peptide ELISA described in this study could serve as a reliable and cost-effective alternative to current methods for serological EHV-1 and EHV-4 diagnosis.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication Date: 2013-08-05 PubMed ID: 23928223DOI: 10.1016/j.jviromet.2013.07.044Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Validation Study
Summary
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The study involves the development of an assay technique, using peptides to distinguish between the antibodies formed in response to two types of equine herpesvirus (EHV-1 and EHV-4). The technique proved effective in identifying horses infected with either type of virus and is seen as promising cost-effective alternative to existing diagnostic methods.
Development of the Peptide ELISA
- The researchers began by creating a peptide-based enzyme-linked immunosorbent assay (ELISA), a test that measures immune response in the body.
- Three and four peptides (short chains of amino acids) were designed for EHV-1 and EHV-4 respectively, and initially studied using sera from experimentally infected foals.
- The most effective pair of peptides, derived from EHV-1 glycoprotein E and EHV-4 glycoprotein G, were chosen for further investigation.
Evaluation of the Peptide ELISA
- The chosen peptide pair was further tested using both acute and convalescent sera, collected from horses that had been infected with either virus either artificially or naturally.
- The researchers also tested ten pairs of pre- and post-vaccination horse sera.
- Results from this phase of the experiment showed the test could successfully identify the type of equine herpesvirus a horse had been infected with. The test was able to distinguish between the two types based on the different immune responses generated against each virus.
Application and Future Validation
- The final part of the study saw the researchers apply the peptide ELISA to a set of samples collected from the field. This test phase demonstrated the technique’s robustness in identifying the virus type based on antibody status.
- This includes detecting type-specific seroconversion for EHV-1 in vaccinated animals, a crucial step in determining whether or not a vaccine is effective.
- Given the success of this experiment, the researchers suggest that with further validation, the new EHV-1/EHV-4 peptide ELISA could eventually serve as a cost-effective and reliable replacement for current methods of serological EHV-1 and EHV-4 diagnosis.
Cite This Article
APA
Lang A, de Vries M, Feineis S, Müller E, Osterrieder N, Damiani AM.
(2013).
Development of a peptide ELISA for discrimination between serological responses to equine herpesvirus type 1 and 4.
J Virol Methods, 193(2), 667-673.
https://doi.org/10.1016/j.jviromet.2013.07.044 Publication
Researcher Affiliations
- Institut für Virologie, Zentrum für Infektionsmedizin, Robert von Ostertag-Haus, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany.
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Enzyme-Linked Immunosorbent Assay / methods
- Herpesviridae Infections / diagnosis
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Herpesvirus 1, Equid / immunology
- Herpesvirus 4, Equid / immunology
- Horse Diseases / diagnosis
- Horse Diseases / virology
- Horses
- Peptides
- Sensitivity and Specificity
- Serologic Tests / methods
Citations
This article has been cited 11 times.- Aguilar-Montes de Oca S, Montes-de-Oca-Jiménez R, Carlos Vázquez-Chagoyán J, Barbabosa-Pliego A, Eliana Rivadeneira-Barreiro P, C Zambrano-Rodríguez P. The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review. Vet Sci 2022 Oct 12;9(10).
- El Brini Z, Fassi Fihri O, Paillot R, Lotfi C, Amraoui F, El Ouadi H, Dehhaoui M, Colitti B, Alyakine H, Piro M. Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations. Animals (Basel) 2021 Sep 29;11(10).
- Pavulraj S, Eschke K, Theisen J, Westhoff S, Reimers G, Andreotti S, Osterrieder N, Azab W. Equine Herpesvirus Type 4 (EHV-4) Outbreak in Germany: Virological, Serological, and Molecular Investigations. Pathogens 2021 Jun 25;10(7).
- Zhang Y, Yang Z, Tian S, Li B, Feng T, He J, Jiang M, Tang X, Mei S, Li H, Zhong Y, Li G, Tang M, Liu S, Tang T, Wang C, Wang X. A newly identified linear epitope on non-RBD region of SARS-CoV-2 spike protein improves the serological detection rate of COVID-19 patients. BMC Microbiol 2021 Jun 26;21(1):194.
- Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry. Front Microbiol 2019;10:2668.
- Azab W, Bedair S, Abdelgawad A, Eschke K, Farag GK, Abdel-Raheim A, Greenwood AD, Osterrieder N, Ali AAH. Detection of equid herpesviruses among different Arabian horse populations in Egypt. Vet Med Sci 2019 Aug;5(3):361-371.
- Qian K, Tian X, Shao H, Ye J, Yao Y, Nair V, Qin A. Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease. BMC Vet Res 2018 Sep 26;14(1):295.
- Ma F, Zhang L, Wang Y, Lu R, Hu B, Lv S, Xue X, Li X, Ling M, Fan S, Zhang H, Yan X. Development of a Peptide ELISA for the Diagnosis of Aleutian Mink Disease. PLoS One 2016;11(11):e0165793.
- Abdelgawad A, Hermes R, Damiani A, Lamglait B, Czirják GÁ, East M, Aschenborn O, Wenker C, Kasem S, Osterrieder N, Greenwood AD. Comprehensive Serology Based on a Peptide ELISA to Assess the Prevalence of Closely Related Equine Herpesviruses in Zoo and Wild Animals. PLoS One 2015;10(9):e0138370.
- El Brini Z, Cullinane A, Garvey M, Fassi Fihri O, Fellahi S, Amraoui F, Loutfi C, Sebbar G, Paillot R, Piro M. First Molecular and Phylogenetic Characterization of Equine Herpesvirus-1 (EHV-1) and Equine Herpesvirus-4 (EHV-4) in Morocco. Animals (Basel) 2025 Jan 5;15(1).
- Ji Y, Zhao X, Liu W. Detection of equine herpesvirus antibodies in large-scale donkey farms in Liaocheng area. Vet Med Sci 2024 Sep;10(5):e70016.
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