Diagnosis of equine piroplasmosis in Brazil by serodiagnostic methods with recombinant antigens.
Abstract: Serum samples from horses in the States of Sao Paulo and Mato Grosso do Sul, Brazil were examined for diagnosis of equine piroplasmosis by both the latex agglutination test (LAT) and enzyme-linked immunosorbent assay (ELISA) with recombinant antigens. Of the 47 samples analyzed, 38 (81%) and 42 (90%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 35 (75%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in the States of Sao Paulo and Mato Grosso do Sul, Brazil.
Publication Date: 2001-11-21 PubMed ID: 11714038DOI: 10.1292/jvms.63.1159Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study presents the diagnosis of a disease called equine piroplasmosis in horses from certain regions in Brazil, which was accomplished using the latex agglutination test and enzyme-linked immunosorbent assay with recombinant antigens. The study found a high prevalence of the disease, emphasizing its seriousness in these regions.
Study Location and Sample
- The research was conducted in two states of Brazil: Sao Paulo and Mato Grosso do Sul.
- For the purpose of the study, a total of 47 samples were collected and examined from horse in these states.
Methods of Diagnosis
- Equine piroplasmosis, the disease under study, was diagnosed using two serodiagnostic methods: the latex agglutination test (LAT) and the enzyme-linked immunosorbent assay (ELISA) with recombinant antigens.
- LAT and ELISA are both widely-used diagnostic tests that identify specific proteins (antigens in this case) in a sample using antibodies. The tests are known for their sensitivity and specificity in diagnosing disease.
Results and Implications
- The study found that out of the 47 samples, 38 (81%) were positive for B. equi infection and 42 (90%) were positive for B. caballi infection, both of which are causative agents of equine piroplasmosis.
- Furthermore, 35 samples (75%) were positive for both B. equi and B. caballi infections, indicating the presence of multiple infections in the same horse.
- These high prevalence rates reveal that equine piroplasmosis is a widespread disease in Sao Paulo and Mato Grosso do Sul, and hence is a concern for the health of horses in these regions.
- The findings of the study emphasize the need for awareness, prevention and control of equine piroplasmosis. They could also aid in the development of future disease management strategies in these regions.
Cite This Article
APA
Xuan X, Nagai A, Battsetseg B, Fukumoto S, Makala LH, Inoue N, Igarashi I, Mikami T, Fujisaki K.
(2001).
Diagnosis of equine piroplasmosis in Brazil by serodiagnostic methods with recombinant antigens.
J Vet Med Sci, 63(10), 1159-1160.
https://doi.org/10.1292/jvms.63.1159 Publication
Researcher Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
MeSH Terms
- Animals
- Antibodies, Protozoan / blood
- Antigens, Protozoan
- Babesia / isolation & purification
- Babesiosis / diagnosis
- Babesiosis / parasitology
- Babesiosis / veterinary
- Brazil
- Enzyme-Linked Immunosorbent Assay / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / immunology
- Horse Diseases / parasitology
- Horses
- Latex Fixation Tests / veterinary
- Recombinant Proteins
Citations
This article has been cited 11 times.- Tirosh-Levy S, Mazuz ML, Savitsky I, Pinkas D, Gottlieb Y, Steinman A. Serological and Molecular Prevalence of Babesia caballi in Apparently Healthy Horses in Israel. Pathogens 2021 Apr 8;10(4).
- Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
- Díaz-Sánchez AA, Pires MS, Estrada CY, Cañizares EV, Del Castillo Domínguez SL, Cabezas-Cruz A, Rivero EL, da Fonseca AH, Massard CL, Corona-González B. First molecular evidence of Babesia caballi and Theileria equi infections in horses in Cuba. Parasitol Res 2018 Oct;117(10):3109-3118.
- Montes Cortés MG, Fernández-García JL, Habela Martínez-Estéllez MÁ. Seroprevalence of Theileria equi and Babesia caballi in horses in Spain. Parasite 2017;24:14.
- Mahmoud MS, El-Ezz NT, Abdel-Shafy S, Nassar SA, El Namaky AH, Khalil WK, Knowles D, Kappmeyer L, Silva MG, Suarez CE. Assessment of Theileria equi and Babesia caballi infections in equine populations in Egypt by molecular, serological and hematological approaches. Parasit Vectors 2016 May 4;9:260.
- Wang M, Guo W, Igarashi I, Xuan X, Wang X, Xiang W, Jia H. Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays. J Vet Med Sci 2014 Apr;76(4):549-52.
- Karatepe B, Karatepe M, Cakmak A, Karaer Z, Ergün G. Investigation of seroprevalence of Theileria equi and Babesia caballi in horses in Nigde province, Turkey. Trop Anim Health Prod 2009 Jan;41(1):109-13.
- Salim BO, Hassan SM, Bakheit MA, Alhassan A, Igarashi I, Karanis P, Abdelrahman MB. Diagnosis of Babesia caballi and Theileria equi infections in horses in Sudan using ELISA and PCR. Parasitol Res 2008 Oct;103(5):1145-50.
- Huang X, Xuan X, Verdida RA, Zhang S, Yokoyama N, Xu L, Igarashi I. Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses. Clin Vaccine Immunol 2006 May;13(5):553-5.
- Huang X, Xuan X, Yokoyama N, Xu L, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Igarashi I. High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis. J Clin Microbiol 2003 Mar;41(3):1147-51.
- Silva-Ramos CR, Niño Rodríguez JA, Gil-Mora J, Betancourt-Ruiz P, Martínez-Díaz HC, Forero-Becerra E, Matiz-González JM, Bolaños E, Olaya-M LA, Benavides E, Hidalgo M. Babesia spp. in Domestic Animals from Rural Areas of Cauca Department: Previous Exposure and Molecular Detection Among Canines, Bovines and Equines. Acta Parasitol 2025 Nov 14;70(6):225.
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