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Journal of veterinary science2020; 21(4); e54; doi: 10.4142/jvs.2020.21.e54

DNA microarray-based characterization and antimicrobial resistance phenotypes of clinical MRSA strains from animal hosts.

Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of severe infections in humans and animals worldwide. Studies elucidating the population structure, staphylococcal cassette chromosome mec types, resistance phenotypes, and virulence gene profiles of animal-associated MRSA are needed to understand spread and transmission. Objective: The objective of this study was to determine 1) clonal complexes and spa types, 2) resistance phenotypes, and 3) virulence/resistance gene profiles of MRSA isolated from animals in Switzerland. Methods: We analyzed 31 presumptive MRSA isolates collected from clinical infections in horses, dogs, cattle, sheep, and pigs, which had tested positive in the Staphaurex Latex Agglutination Test. The isolates were characterized by spa typing and DNA microarray profiling. In addition, we performed antimicrobial susceptibility testing using the VITEK 2 Compact system. Results: Characterization of the 31 presumptive MRSA isolates revealed 3 methicillin-resistant Staphylococcus pseudintermedius isolates, which were able to grow on MRSA2 Brilliance agar. Of the 28 MRSA isolates, the majority was assigned to CC398 (86%), but CC8 (11%) and CC1 (4%) were also detected. The predominant spa type was t011 (n = 23), followed by t009 (n = 2), t034 (n = 1), t008 (n = 1), and t127 (n = 1). Conclusions: The results of this study extend the current body of knowledge on the population structure, resistance phenotypes, and virulence and resistance gene profiles of MRSA from livestock and companion animals.
© 2020 The Korean Society of Veterinary Science.
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Publication Date: 2020-08-01 PubMed ID: 32735092PubMed Central: PMC7402938DOI: 10.4142/jvs.2020.21.e54Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on characterizing Methicillin-resistant Staphylococcus aureus (MRSA) strains from animal hosts in Switzerland and determining their resistance phenotypes.

Objective of the Study

  • The main aim of this study was to understand the structure, resistance phenotype, and gene profiles of MRSA strains that have been isolated from various animals. The animals included horses, dogs, sheep, cattle, and pigs.

Methods

  • The research methodology employed in this study involved analyzing 31 presumptive MRSA isolates. This was achieved by using Staphaurex Latex Agglutination Test to confirm the positivity of the isolates.
  • Following this, characterization of these isolates was undertaken by using MLST typing and DNA microarray profiling.
  • Additionally, antimicrobial susceptibility testing was also carried out utilizing the VITEK 2 Compact system.

Results

  • The results indicated the presence of three methicillin-resistant S. aureus isolates that were cultivable on MRSA2 Brilliance agar.
  • Of the 28 other MRSA isolates, the dominant was assigned to the clone complex CC398 (86%), followed by CC8 (11%), and CC1 (4%).
  • Furthermore, the predominant spa type was t011, followed by t009, t034, t008, and t127.

Conclusions

  • The findings from this study augment the existing understanding of the population structure, resistance phenotypes, and virulence and resistance gene profiles of MRSA strains in livestock and companion animals.
  • This could enhance the management and control strategies for MRSA infections in both human and animal populations. It also has implications for antimicrobial drug design and policy making in the realm of animal health management.

Cite This Article

APA
Schmitt S, Stephan R, Huebschke E, Schaefle D, Merz A, Johler S. (2020). DNA microarray-based characterization and antimicrobial resistance phenotypes of clinical MRSA strains from animal hosts. J Vet Sci, 21(4), e54. https://doi.org/10.4142/jvs.2020.21.e54

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 21
Issue: 4
Pages: e54

Researcher Affiliations

Schmitt, Sarah
  • Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
Stephan, Roger
  • Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
Huebschke, Ella
  • Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
Schaefle, Daniel
  • Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
Merz, Axel
  • Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland.
Johler, Sophia
  • Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, 8057 Zurich, Switzerland. sophia.johler@uzh.ch.

MeSH Terms

  • Animals
  • Cattle
  • Cattle Diseases / microbiology
  • Dog Diseases / microbiology
  • Dogs
  • Drug Resistance, Microbial / genetics
  • Horse Diseases / microbiology
  • Horses
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Methicillin-Resistant Staphylococcus aureus / genetics
  • Oligonucleotide Array Sequence Analysis / veterinary
  • Phenotype
  • Sheep
  • Sheep Diseases / microbiology
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / veterinary
  • Swine
  • Swine Diseases / microbiology

Conflict of Interest Statement

The authors declare no conflicts of interest.

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