DNases in milk and blood sera from different species.
Abstract: DNases were demonstrated in samples of colostrum and blood serum from man and various domestic animals. The measurable DNase activity recorded was highest in samples from cat and dog and lowest in samples from goat, horse, pig and sheep. In contrast to DNases produced by certain bacteria, these enzymes were thermo-labile and the activity was maximal in the area pH 5.0–5.5. A modification of an agar medium originally described for the demonstration of bacterial DNases was found to be suitable for assays of DNases from colostrum, milk and serum. DNaser ble påvist i prøver fra kolostrum og blodserum fra menneske og forskjellige husdyr. DNaseaktiviteten var høyest i prøver fra hund og katt og lavest i prøver fra geit, hest, gris og sau. I mot-setning til DNaser produsert av visse bakterier, var disse enzymene termolabile og aktiviteten var størst i pH-området 5,0 til 5,5. En modifikasjon av et agarmedium beskrevet for påvisning av bakterielle DNaser ble funnet å være velegnet ved undersøkelse av DNaser fra kolostrum, melk og serum.
Publication Date: 1979-01-01 PubMed ID: 116487PubMed Central: PMC8322905DOI: 10.1186/BF03546602Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the presence and activity of Deoxyribonuclease (DNase) enzymes in the colostrum and blood serum of humans and different domestic animals. Results show DNase activity to be highest in cat and dog samples, and lowest in those from goat, horse, pig and sheep.
DNases in Different Species
- The study focused on the presence and activity of DNases, enzymes that catalyze the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, in the colostrum and blood serum of humans and various domestic animals.
- Among the animals studied, cats and dogs exhibited the highest levels of measurable DNase activity, while the lowest levels were found in the samples from goats, horses, pigs, and sheep.
Characteristics of DNases Found
- The DNases found in the studied samples were characterized as being thermo-labile, meaning they are sensitive to high temperatures, much unlike the DNases produced by certain types of bacteria.
- Furthermore, the DNase activity was found to be most prominent in the pH range of 5.0 to 5.5, highlighting the acidic milieu under which these enzymes function optimally.
Detection Method for DNases
- A modified variant of an agar medium, originally described for detecting bacterial DNases, was discovered to be suitable for assays of DNases in colostrum, milk, and serum. This demonstrates the robustness of this method for detecting DNases across different types of biological samples.
In conclusion, this research demonstrates the presence and measures the activity of DNases in different species, revealing a unique pattern across species that might further our understanding of these important enzymes and their roles in various biological processes and diseases.
Cite This Article
APA
Gudding R.
(1979).
DNases in milk and blood sera from different species.
Acta Vet Scand, 20(3), 404-416.
https://doi.org/10.1186/BF03546602 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cats
- Deoxyribonucleases / analysis
- Deoxyribonucleases / blood
- Dogs
- Goats
- Horses
- Humans
- Immunodiffusion
- Isoelectric Focusing
- Milk / enzymology
- Milk, Human / enzymology
- Sheep
- Species Specificity
- Spectrophotometry
- Swine
References
This article includes 17 references
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Citations
This article has been cited 5 times.- Gudding R. Staphylococcal nuclease in udder secretions of cows with acute mastitis.. Acta Vet Scand 1980;21(1):79-95.
- Gudding R. Nucleases of some udder pathogenic organisms. In vivo and in vitro production.. Acta Vet Scand 1980;21(2):256-66.
- Gudding R. Differentiation of staphylococci on the basis of nuclease properties.. J Clin Microbiol 1983 Nov;18(5):1098-101.
- Høie S, Gudding R. A method for monitoring antibodies against staphylococcal DNases.. Acta Vet Scand 1990;31(2):223-6.
- Gudding R. An agar diffusion method for the determination of antibodies against Staphylococcus aureus deoxyribonuclease.. Acta Vet Scand 1977;18(4):480-93.
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