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Open veterinary journal2024; 14(8); 2057-2072; doi: 10.5455/OVJ.2024.v14.i8.35

Effect of hCG and prostaglandin on ovarian, luteal development, and hormonal changes in embryo donor mares during the hot summer months in subtropics.

Abstract: Commercial embryo flushing of horses has required hormonal management of both the donor and recipient mares throughout the breeding season. Unassigned: This study aimed to find out the effect of using human chorionic gonadotropin (hCG) and prostaglandin F2α (PG) on the ovarian and uterine dynamics and hemodynamics, estradiol (E2), progesterone, oxidants-antioxidants, and blood biochemicals in embryo donor mares during the hottest months of the year in a subtropical climate. Unassigned: Three Control estrous cycles of native mares (10-20 years; = 10) followed by two treated cycles with hCG and PGF2α were examined daily from May to August using Doppler ultrasound with blood sampling. Circulating, progesterone (P4), total cholesterol, total proteins, albumin, haptoglobin, nitric oxide (NO), catalase, alkaline phosphatase, lactate dehydrogenase (LDH), and myeloperoxidase were measured in blood serum. Unassigned: Days during the control estrous cycle impacted the dominant follicle (DF) diameter ( < 0.0001), antrum diameter ( < 0.0001), area ( < 0.0001), antral area ( 0.05), and corpus luteum (CL) diameter ( 0.05) but influenced its antrum diameter ( < 0.05), color area ( < 0.05), CL diameter ( 0.05) and the antrum area ( > 0.05), but influenced CL diameter ( < 0.0001). PGF2α and hCG increased uterine horn area ( = 0.016) and color area ( = 0.023), total cholesterol ( < 0.0001), and NO ( < 0.0001) levels but hCG increased the levels of myeloperoxidase ( < 0.005), total proteins ( < 0.001), and albumin ( < 0.0001). Globulins achieved the highest level ( = 0.054) but the Albumin/globulin ratio reached a minimum value on Day 0 of the control mares ( < 0.0001). PGF2α increased LDH ( < 0.0001) and sharply declined ( = 0.028) progesterone. Unassigned: In conclusion, the treatment protocols of hCG and PGF2α showed minimal effects on the produced ovulating follicles and can be used during the summer season to manage embryo donor mares.
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Publication Date: 2024-08-31 PubMed ID: 39308718PubMed Central: PMC11415914DOI: 10.5455/OVJ.2024.v14.i8.35Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how the use of human chorionic gonadotropin (hCG) and prostaglandin F2α (PG) affect the ovaries and hormonal changes in embryo donor horses in hot subtropical climates.

Study Objective and Methods

  • The research aimed to understand the influence of hCG and PG on ovary and uterus dynamics, hemodynamics, estradiol, progesterone, oxidants-antioxidants, and blood biochemicals in donor mares during the hottest part of the year in a subtropical environment.
  • Three control estrous cycles of native mares, aged between 10 to 20 years, were first studied, followed by two cycles treated with hCG and PGF2α.
  • This study was conducted from May to August and involved daily assessments made using Doppler ultrasound and blood sampling.

Evaluating the Effects

  • Various hormones, enzymes, and biochemicals in the blood serum such as progesterone, total cholesterol, total proteins, albumin, haptoglobin, nitric oxide, catalase, alkaline phosphatase, lactate dehydrogenase, and myeloperoxidase were measured.
  • Several factors such as the diameter of the dominant follicle (DF), antrum diameter, area, antral area, and corpus luteum (CL) diameter were found to be influenced by the number of days in the control estrous cycle.
  • The impact of PG was observed on the DF and its diameter, color area, and CL diameter.
  • Days after hCG showed an influence on DF, antrum diameter, and the antrum area, while significantly affecting the diameter of the corpus luteum.

Findings

  • Both PGF2α and hCG increased the uterine horn area and color area, total cholesterol, and nitric oxide levels.
  • hCG was particularly noted to increase the levels of myeloperoxidase, total proteins, and albumin.
  • Globulins reached their highest level, and the Albumin/globulin ratio its minimum value on Day 0 of the control mares.
  • PGF2α was observed to increase lactate dehydrogenase and sharply decrease progesterone.

Conclusion

  • The researchers concluded that the treatment protocols of hCG and PGF2α had minimal effects on the produced ovulating follicles, suggesting that they can be used to manage embryo donor mares during the summer season.

Cite This Article

APA
Alkhadrawy JMH, Aboelmaaty AM, Abou-Ahmed MM, Ghallab AM. (2024). Effect of hCG and prostaglandin on ovarian, luteal development, and hormonal changes in embryo donor mares during the hot summer months in subtropics. Open Vet J, 14(8), 2057-2072. https://doi.org/10.5455/OVJ.2024.v14.i8.35

Publication

Open veterinary journal
ISSN: 2218-6050
NlmUniqueID: 101653182
Country: Libya
Language: English
Volume: 14
Issue: 8
Pages: 2057-2072

Researcher Affiliations

Alkhadrawy, Jamal Mohamed Hassan
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
  • National Center of Animal Health, Ministry of Agriculture, Livestock and Marine Resources, Tripoli, Libya.
Aboelmaaty, Amal Mahmoud
  • Animal Reproduction and AI department, Veterinary Research Institute, National Research Centre, Giza, Egypt.
Abou-Ahmed, Mostafa Mohamed
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Ghallab, Abdelraouf Morsy
  • Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

MeSH Terms

  • Animals
  • Female
  • Horses / physiology
  • Horses / blood
  • Chorionic Gonadotropin / pharmacology
  • Chorionic Gonadotropin / administration & dosage
  • Dinoprost / administration & dosage
  • Dinoprost / pharmacology
  • Ovary / drug effects
  • Ovary / physiology
  • Seasons
  • Corpus Luteum / drug effects
  • Corpus Luteum / physiology
  • Progesterone / blood
  • Embryo Transfer / veterinary
  • Estradiol / blood

Conflict of Interest Statement

The authors declare that they do not have any conflict of interest.

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