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Home / Equine Research Bank / J Vet Intern Med / Effect of leukoreduction on the metabolism of equine packed ...
Journal of veterinary internal medicine2024; doi: 10.1111/jvim.17015

Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage.

Abstract: Understanding of the biochemical and morphological lesions associated with storage of equine blood is limited. Objective: To demonstrate the temporal sequences of lipid and metabolic profiles of equine fresh and stored (up to 42 days) and leukoreduced packed red blood cells (LR-pRBC) and non-leukoreduced packed RBC (nLR-pRBC). Methods: Packed RBC units were obtained from 6 healthy blood donor horses enrolled in 2 blood banks. Methods: Observational study. Whole blood was collected from each donor using transfusion bags with a LR filter. Leukoreduction pRBC and nLR-pRBC units were obtained and stored at 4°C for up 42 days. Sterile weekly sampling was performed from each unit for analyses. Results: Red blood cells and supernatants progressively accumulated lactate products while high-energy phosphate compounds (adenosine triphosphate and 2,3-Diphosphoglycerate) declined. Hypoxanthine, xanthine, and free fatty acids accumulated in stored RBC and supernatants. These lesions were exacerbated in non-LR-pRBC. Conclusions: Leukoreduction has a beneficial effect on RBC energy and redox metabolism of equine pRBC and the onset and severity of the metabolic storage lesions RBC.
© 2024 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2024-02-26 PubMed ID: 38406982DOI: 10.1111/jvim.17015Google Scholar: Lookup
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Summary

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The research explores how leukoreduction affects the metabolism of horse red blood cells during storage. The study found that leukoreduced packed red blood cells fared better in their energy and redox metabolism, and experienced less severe metabolic storage damage, compared to non-leukoreduced ones.

Introduction

  • The study draws focus to understanding the biochemical and morphological damages associated with storing horse blood, an area where knowledge is currently limited.

Objective

  • The aim was to observe the sequence of changes in the lipid and metabolic profiles of two types of horse red blood cells – fresh and stored (for up to 42 days), leukoreduced packed red blood cells (LR-pRBC), and non-leukoreduced packed red blood cells (nLR-pRBC).

Method

  • RBC units were obtained from six healthy blood donor horses, registered in two blood banks.
  • Whole blood was collected from each donor using transfusion bags with a leukoreduction filter.
  • The two types of red blood cells, LR-pRBC and nLR-pRBC units, were then extracted and stored at 4°C for up to 42 days.
  • Sterile weekly sampling was conducted to analyse each unit.

Results

  • As storage time progressed, an accumulation of lactate products was observed in both red blood cells and supernatants, while high-energy phosphate compounds declined.
  • Hypoxanthine, xanthine, and free fatty acids were also noted to accumulate in stored red blood cells and supernatants.
  • These damages were more pronounced in non-leukoreduced RBCs.

Conclusions

  • The process of leukoreduction was found to have a positive effect on the energy and redox metabolism of equine RBCs.
  • It was also found to delay the onset and lessen the severity of metabolic storage damages.

Cite This Article

APA
Miglio A, Rocconi F, Cremonini V, D'Alessandro A, Reisz JA, Maslanka M, Lacroix IS, Tiscar G, Di Tommaso M, Antognoni MT. (2024). Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage. J Vet Intern Med. https://doi.org/10.1111/jvim.17015

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English

Researcher Affiliations

Miglio, Arianna
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Rocconi, Francesca
  • Department of Veterinary Medicine, Veterinary University Hospital, University of Teramo, Teramo, Italy.
Cremonini, Valentina
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
D'Alessandro, Angelo
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver-Anschutz Medical Campus, Aurora, Colorado, USA.
Reisz, Julie A
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver-Anschutz Medical Campus, Aurora, Colorado, USA.
Maslanka, Mark
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver-Anschutz Medical Campus, Aurora, Colorado, USA.
Lacroix, Ian S
  • Department of Biochemistry and Molecular Genetics, University of Colorado Denver-Anschutz Medical Campus, Aurora, Colorado, USA.
Tiscar, Giorgio
  • Department of Veterinary Medicine, Veterinary University Hospital, University of Teramo, Teramo, Italy.
Di Tommaso, Morena
  • Department of Veterinary Medicine, Veterinary University Hospital, University of Teramo, Teramo, Italy.
Antognoni, Maria T
  • Department of Veterinary Medicine, University of Perugia, Perugia, Italy.

Grant Funding

  • R01HL146442 / National Heart, Lung, and Blood Institute (AD, SLS)
  • R01HL149714 / National Heart, Lung, and Blood Institute (AD, SLS)
  • R01HL148151 / National Heart, Lung, and Blood Institute (AD, SLS)
  • R21HL150032 / National Heart, Lung, and Blood Institute (AD, SLS)

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