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Home / Equine Research Bank / J Vet Intern Med / Effects of intravenous administration of ascorbic acid (vita...
Journal of veterinary internal medicine2023; doi: 10.1111/jvim.16934

Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses.

Abstract: Ascorbic acid (AA) is an antioxidant that might be beneficial for adjunctive treatment of sepsis in horses. The optimal dose and effects on oxidative status are unknown. Objective: Ascorbic acid administration will increase plasma AA concentrations and decrease determinants of reactive oxygen metabolites (dROM), basal and stimulant-induced intraerythrocytic reactive oxygen species (ROS) concentrations, and stimulant-induced neutrophil ROS production, and increase plasma antioxidant capacity (PAC) in a dose-dependent manner. Methods: Eight healthy horses. Methods: Randomized placebo-controlled crossover study. Each horse received 4 single-dose IV treatments including AA at 25, 50, and 100 mg/kg and saline (placebo) with each treatment separated by ≥1 week. Blood was collected at baseline, 2 and 6 hours for assessment of plasma dROM and PAC via photometer, intraerythrocytic ROS by flow cytometry, and stimulant-induced neutrophil ROS by a fluorometric assay. Plasma AA concentrations were measured by high-performance liquid chromatography/electrochemical detection. Results: Ascorbic acid at 100 mg/kg resulted in decreased dROM 2 hours after treatment (P = .03, 95% CI 5.51-121.2, point estimate 63.3). There was no effect of AA on basal or stimulant-induced intraerythrocytic ROS (P = .88, 95% CI -0.156 to 0.081, point estimate -0.037; P = .93, 95% CI -0.123 to 0.112, point estimate -0.006, respectively), basal or stimulant-induced neutrophil ROS (P ≥ .12, 95% CI -644.9 to 56.2, point estimate -294.4), or PAC (P ≥ .64, 95% CI -1567 to 463.4, point estimate -552.0) at any dose or timepoint. Plasma AA concentrations increased in a dose-dependent manner. Conclusions: High-dose administration of AA might provide antioxidant benefits in horses.
© 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2023-11-10 PubMed ID: 37948618DOI: 10.1111/jvim.16934Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the impact of vitamin C, also known as ascorbic acid (AA), on the oxidative status in horses, essentially looking at how various doses of AA can modify the manner in which horse cells respond to potentially harmful substances known as reactive oxygen species (ROS). The conclusions drawn from the study show that high dosages of AA might lead to antioxidant benefits in horses.

Research Objectives and Procedure

  • The study sought to determine the optimal dose of AA for horses and its effects on their oxidative status. It aimed to reveal whether the administration of ascorbic acid could not only increase plasma AA concentrations but also decrease the quantity of ROS while improving plasma antioxidant capacity (PAC).
  • To test this hypothesis, a randomized placebo-controlled study was carried out involving eight healthy horses. Each horse received four single-dose intravenous treatments which included AA at doses of 25, 50, and 100 mg/kg and saline as a placebo. The treatments were all separated by a minimum of one week.
  • The researchers then collected blood samples from the horses at baseline, 2 and 6 hours following the treatments. The team assessed levels of ROS and PAC alongside plasma AA concentrations through a series of lab tests.

Research Findings

  • The results indicated that AA administered at a dose of 100 mg/kg led to a decrease in reactive oxygen metabolites (dROM) after 2 hours. There was, however, no significant impact on basal or stimulant-induced intraerythrocytic ROS and neutrophil ROS, or PAC at any dosage or time evaluated.
  • One significant observation was that plasma AA concentrations increased in a dose-dependent manner, which means higher doses led to increased levels of Vitamin C in the blood.
  • Therefore, the study concludes that high-dose administration of AA might provide antioxidant benefits in horses, thus improving their oxidative status and potentially aiding in protection against cell damage.

Cite This Article

APA
Taylor SD, Hart KA, Vaughn S, Giancola SC, Serpa PBS, Santos AP. (2023). Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses. J Vet Intern Med. https://doi.org/10.1111/jvim.16934

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English

Researcher Affiliations

Taylor, Sandra D
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.
Hart, Kelsey A
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Vaughn, Sarah
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Giancola, Shyla C
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Serpa, Priscila B S
  • Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.
Santos, Andrea P
  • Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.

Grant Funding

  • 1016369 / National Institute of Food and Agriculture

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