Effects of live and pasteurized forms of Lactobacillus casei Zhang on acute kidney injury and chronic renal fibrosis.
Abstract: Lactobacillus casei Zhang (Lac.z), isolated from traditional sour horse milk in Inner Mongolia, can alleviate various diseases and promote health. Our previous studies found that pretreatment with live Lac.z (L-Lac.z) could significantly attenuate acute kidney injury and delay the progression of chronic renal fibrosis. However, it is unknown whether these effects could be maintained by pasteurized Lac.z (P-Lac.z). Mouse models of acute kidney injury and chronic renal fibrosis induced by renal bilateral ischemia-reperfusion (BIR) surgery were treated with L-Lac.z or P-Lac.z by gavage. Serum and kidney samples were collected to analyze the extent of renal injury and fibrosis, and proteomics was used to explore the potential mechanisms underlying the differences in the effects of the two forms of Lac.z. The results revealed that treatment with L-Lac.z led to a reduction in serum urea nitrogen levels and in less renal tubular injury and subsequent renal fibrosis after BIR-induced renal injury, whereas these effects were not observed in the P-Lac.z group. Proteomic analysis revealed 19 up-regulated proteins and 39 down-regulated proteins in the P-Lac.z group, and these gene products were associated with growth and stress resistance. The specific nephroprotective effects of L-Lac.z may be independent of the interaction of live probiotics with the host.
© 2024. The Author(s).
Publication Date: 2024-08-26 PubMed ID: 39222221PubMed Central: 4931701DOI: 10.1007/s42770-024-01491-yGoogle Scholar: Lookup
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Summary
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The research article investigates the effect of both live and pasteurized Lactobacillus casei Zhang (a bacteria strain) on acute kidney injury and chronic renal fibrosis. It confirms the beneficial impact of pretreatment with the live form but finds no such benefits in the pasteurized version.
Objective and Methodology
- The aim of the study was to compare the effects of live and pasteurized forms of a bacteria strain known as Lactobacillus casei Zhang (Lac.z) on acute kidney injury and chronic renal fibrosis.
- This bacteria has previously shown potential in preventing and managing these kidney conditions when administered in a live form.
- However, whether the benefits extend to its pasteurized form (a process which kills bacteria but maintains some of its properties) was not known. Hence, the study sought to test the effects of both versions.
- The researchers did this by introducing both forms of the bacteria to mouse models that had induced kidney injuries. The subjects were treated via gavage (oral cramming of food treatment into the stomach).
- After treatment, the serum and kidney samples were taken from the mice for further analysis of kidney damage and fibrosis to compare the effects of both forms of Lac.z.
Findings
- The results proved that the live Lac.z was effective in reducing serum urea nitrogen levels, meaning less kidney damage and subsequent kidney fibrosis. This was measured after testing the mice for bilateral ischemia-reperfusion injury, a standard method to test kidney problems.
- Unfortunately, these positive effects were not observed in the pasteurized Lac.z group. The severity of the renal injury and fibrosis failed to minimize in the treated mice.
Analysis
- Proteomics (study of proteins) was also used to explore why there was a difference in the impacts of the two forms of the bacteria. This research found that there were 19 up-regulated proteins and 39 down-regulated proteins in the group that was treated with pasteurized Lac.z.
- These protein changes were linked to growth and resistance to stress, suggesting some interaction may have caused the pasteurized Lac.z to be less effective.
- The study therefore concludes that the specific effects of Lac.z in protecting the kidneys are possibly independent of the interaction of live probiotics with the host, emphasizing the potential benefits of treatment with the live form of Lac.z.
Cite This Article
APA
Wang X, Shi M, Cao C, Zeng R, Yao Y.
(2024).
Effects of live and pasteurized forms of Lactobacillus casei Zhang on acute kidney injury and chronic renal fibrosis.
Braz J Microbiol.
https://doi.org/10.1007/s42770-024-01491-y Publication
Researcher Affiliations
- Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
- Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
- Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
- Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China. zengrui@tjh.tjmu.edu.cn.
- Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China. yaoyingkk@126.com.
- Division of Nutrition, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China. yaoyingkk@126.com.
Grant Funding
- 82170701 / National Natural Science Foundation of China
- 82370700 / National Natural Science Foundation of China
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