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Home / Equine Research Bank / Methods Mol Biol / Equine Induced Pluripotent Stem Cell Culture.
Methods in molecular biology (Clifton, N.J.)2023; 2749; 175-184; doi: 10.1007/978-1-0716-3609-1_16

Equine Induced Pluripotent Stem Cell Culture.

Abstract: Groundbreaking work by Takahashi and Yamanaka in 2006 demonstrated that non-embryonic cells can be reprogrammed into pluripotent stem cells (PSCs) by forcing the expression of a defined set of transcription factors in culture, thus overcoming ethical concerns linked to embryonic stem cells. Induced PSCs have since revolutionized biomedical research, holding tremendous potential also in other areas such as livestock production and wildlife conservation. iPSCs exhibit broad accessibility, having been derived from a multitude of cell types and species. Apart from humans, iPSCs hold particular medical promise in the horse. The potential of iPSCs has been shown in a variety of biomedical contexts in the horse. However, progress in generating therapeutically useful equine iPSCs has lagged behind that reported in humans, with the generation of footprint-free iPSCs using non-integrative reprogramming approaches having proven particularly challenging. A greater understanding of the underlying molecular pathways and essential factors required for the generation and maintenance of equine iPSCs and their differentiation into relevant lineages will be critical for realizing their significant potential in veterinary regenerative medicine. This article outlines up-to-date protocols for the successful culture of equine iPSC, including colony selection, expansion, and adaptation to feeder-free conditions.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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Publication Date: 2023-12-22 PubMed ID: 38133784PubMed Central: 4672244DOI: 10.1007/978-1-0716-3609-1_16Google Scholar: Lookup
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Summary

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The research article discusses the protocols and methods to successfully grow and maintain cultures of horse-induced pluripotent stem cells (iPSCs), which can be reprogrammed to regenerate diverse forms of cell types and hold significant potential in veterinary regenerative medicine.

Generation and Relevance of Induced Pluripotent Stem Cells

  • The paper begins by citing the pioneering work by Takahashi and Yamanaka in 2006, which showed the potential of reprogramming non-embryonic cells into pluripotent stem cells (PSCs) by manipulating the expression of a set of transcription factors in culture.
  • Since this discovery, iPSCs have significantly altered the landscape of biomedical research and found applicability in numerous domains such as livestock production and wildlife conservation due to their ability to be derived from a variety of cell types and species.

Challenges and Potential of Equine iPSCs

  • Aside from human cells, horse cells are highlighted as having great medical potential using the iPSC method given the diverse biomedical contexts they’ve been shown to be effective in.
  • However, the authors note that progress in obtaining therapeutically useful equine iPSCs has been slower as compared to human iPSCs.
  • The creation of ‘footprint-free’ iPSCs, which involves non-integrative reprogramming techniques, have been particularly difficult in the horse.

Molecular Understanding and Pathways

  • The paper emphasises that a deeper comprehension of the molecular mechanisms and key factors needed for creation, maintenance and differentiation of equine iPSCs into pertinent lineages will be critical in harnessing their substantial potential in veterinary regenerative medicine.

The Protocol for Equine iPSCs Culture

  • The research provides an update on protocols for the successful culture of equine iPSC, which includes processes such as selecting the right colonies, expansion, and acclimatization to feeder-free conditions. This implies the culture can sustain itself without the need for additional nourishment from feeder cells.

Cite This Article

APA
Falk J, Donadeu FX. (2023). Equine Induced Pluripotent Stem Cell Culture. Methods Mol Biol, 2749, 175-184. https://doi.org/10.1007/978-1-0716-3609-1_16

Publication

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
NlmUniqueID: 9214969
Country: United States
Language: English
Volume: 2749
Pages: 175-184

Researcher Affiliations

Falk, Julia
  • Division of Translational Bioscience, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK. J.Falk-1@sms.ed.ac.uk.
Donadeu, F Xavier
  • Division of Translational Bioscience, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK.

MeSH Terms

  • Humans
  • Horses
  • Animals
  • Induced Pluripotent Stem Cells
  • Pluripotent Stem Cells
  • Cell Differentiation
  • Embryonic Stem Cells
  • Transcription Factors / metabolism
  • Cellular Reprogramming

References

This article includes 19 references
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