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Home / Equine Research Bank / Sci Rep / Erosion behaviour of human, bovine and equine dental hard ti...
Scientific reports2023; 13(1); 19617; doi: 10.1038/s41598-023-46759-9

Erosion behaviour of human, bovine and equine dental hard tissues.

Abstract: Dental hard tissues from different species are used in dental research, but little is known about their comparability. The aim of this study was to compare the erosive behaviour of dental hard tissues (enamel, dentin) obtained from human, bovine and equine teeth. In addition, the protective effect of the pellicle on each hard tissue under erosive conditions was determined. In situ pellicle formation was performed for 30 min on enamel and dentin samples from all species in four subjects. Calcium and phosphate release was assessed during 120 s of HCl incubation on both native and pellicle-covered enamel and dentin samples. SEM and TEM were used to examine surface changes in native enamel and dentin samples after acid incubation and the ultrastructure of the pellicle before and after erosive exposure. In general, bovine enamel and dentin showed the highest degree of erosion after acid exposure compared to human and equine samples. Erosion of human primary enamel tended to be higher than that of permanent teeth, whereas dentin showed the opposite behaviour. SEM showed that eroded equine dentin appeared more irregular than human or bovine dentin. TEM studies showed that primary enamel appeared to be most susceptible to erosion.
© 2023. The Author(s).
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Publication Date: 2023-11-10 PubMed ID: 37949920PubMed Central: PMC10638419DOI: 10.1038/s41598-023-46759-9Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

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The research investigates the comparative erosive behavior of dental hard tissues from human, cow, and horse teeth. Additionally, it analyses the protective role of the pellicle, a protein film on the tooth surface, under conditions of erosion.

Objective of the Study

  • The key objective of the research was to scrutinize the erosion behavior of hard dental tissues – enamel and dentine – from humans, cows, and horses. The study specifically examined how these tissues react to erosion, while also attempting to understand the protection offered by the pellicle, a naturally occurring protein layer, under various erosive conditions.

Methodology of the Study

  • The initial part of the study involved in situ pellicle formation on enamel and dentin samples from all three species, using four subjects over 30 minutes.
  • The research evaluated the release of calcium and phosphate during a two-minute period of hydrochloric acid incubation on both unprotected and pellicle-covered enamel and dentin samples.
  • Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) tools were employed to assess surface changes in unprotected enamel and dentin samples after acid incubation. These tools were used to study the ultrastructure of the pellicle before and after exposure to erosive conditions as well.

Key Findings of the Study

  • The study revealed that bovine enamel and dentin demonstrated the highest level of erosion post acid exposure when compared to human and equine samples.
  • The erosion of enamel from primary human teeth was observed to be higher than that of permanent teeth. However, with dentin, the erosion trend was reversed.
  • The SEM study showed that eroded equine dentin appeared to be more uneven than human or bovine dentin.
  • According to TEM studies, the primary enamel seemed to be the most susceptible to erosion.

Cite This Article

APA
Hertel S, Basche S, Schmidt V, Staszyk C, Hannig C, Sterzenbach T, Hannig M. (2023). Erosion behaviour of human, bovine and equine dental hard tissues. Sci Rep, 13(1), 19617. https://doi.org/10.1038/s41598-023-46759-9

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 19617
PII: 19617

Researcher Affiliations

Hertel, S
  • Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany. susann.hertel@uniklinikum-dresden.de.
Basche, S
  • Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany.
Schmidt, V
  • Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, 66421, Homburg, Germany.
Staszyk, C
  • Institute for Veterinary-Anatomy, -Histology and -Embryology, Faculty for Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Str. 98, 35392, Giessen, Germany.
Hannig, C
  • Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany.
Sterzenbach, T
  • Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307, Dresden, Germany.
Hannig, M
  • Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, 66421, Homburg, Germany.

MeSH Terms

  • Humans
  • Animals
  • Cattle
  • Horses
  • Tooth Erosion
  • Dentin
  • Calcium / pharmacology
  • Hydrochloric Acid / pharmacology
  • Phosphates / pharmacology

Conflict of Interest Statement

The authors declare no competing interests.

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