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Veterinary sciences2020; 7(4); 191; doi: 10.3390/vetsci7040191

Estimation of Nitrite-Nitric Oxide Derivative-In Horses with Intestinal Colic by ESR Spectroscopy.

Abstract: Diseases of the gastrointestinal tract of horses are caused by many factors and have a complex pathogenesis. Developing effective methods of differential diagnostics is of high fundamental and applied importance. The pathogenesis of diseases of the digestive tract of horses accompanied by the development of inflammation and oxidative stress, can be associated with a lack of the nitrogen monoxide which controls many signaling pathways in the body. The level of the nitric oxide (NO) is involved in the regulation of the immune and nervous systems, the tone of all the blood vessels, and the courses of many pathological processes. The nitric oxide activates guanylate cyclase (sGC) and leads to vascular relaxation. The aim of this investigation was to study the metabolites of nitric oxide in horses suffered from intestinal diseases. The levels of nitric oxide in the blood serum of horses depending on their age and health state was studied. The concentration of nitrites in the blood serum of horses aged 6-25 years was 3.4 ± 4.2 μM, and in the young horses (1-5 years) the level of this indicator was 8.2 ± 5.4 μM. A sharp decrease in nitrite was observed in all the horses with intestinal diseases of 2 ± 0.9 μM, especially with tympanitic caecun of 0.6 ± 0.4 μM and with spasmodic colic of 1.8 ± 0.5 μM. The level of nitrosylhemoglobin HbNO in the blood of the diseased animals was higher than that in clinically healthy horses, regardless of age.
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Publication Date: 2020-11-29 PubMed ID: 33260335PubMed Central: PMC7712281DOI: 10.3390/vetsci7040191Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on investigating the levels of nitrite-nitric oxide derivative in horses suffering from intestinal diseases such as colic. The key findings are a decreased level of nitrites in horses with intestinal conditions, and elevated levels of nitrosylhemoglobin in affected animals compared to healthy ones, regardless of their age.

Objective and Importance of the Research

  • The research aimed to examine the metabolites of nitric oxide in horses affected by intestinal diseases. This is critical as a lack of nitrogen monoxide or nitric oxide (NO), which regulates various bodily functions such as immune and nervous systems and blood vessel tone, can lead to inflammation and oxidative stress forming part of the complex pathogenesis of digestive tract diseases in horses.
  • Establishing effective diagnostic methods for these conditions, especially with the view of nitric oxide metabolites, is deemed to be of high fundamental and applied significance in veterinary practice.

Investigation and Findings

  • The researchers studied the levels of nitric oxide in the blood serum of horses of different ages and health statuses. They found that in horses aged 6 to 25 years, the nitrite concentration was 3.4 ± 4.2 μM, whereas in younger horses aged 1 to 5 years, it was significantly higher, reaching 8.2 ± 5.4 μM.
  • However, in case of horses suffering from intestinal diseases, a significant drop in nitrite levels was noticed, registering at 2 ± 0.9 μM across all categories. This reduction was particularly sharp for those with tympanitic caecun and spasmodic colic, measuring 0.6 ± 0.4 μM and 1.8 ± 0.5 μM respectively.
  • Moreover, the level of nitrosylhemoglobin (HbNO) was found to be higher in the blood of sick horses compared to that in clinically healthy ones, irrespective of their age. This suggests an interaction pathway affected by intestinal disorders, potentially as a compensatory mechanism.

Implications of the Research

  • The outcomes of this study provide useful insights into the role of nitric oxide metabolites in the pathogenesis of horse intestinal diseases, highlighting their potential as diagnostic markers.
  • The significant variations in levels of nitrite and HbNO in affected horses compared to healthy ones point towards the involvement of these components in gastrointestinal conditions, supporting the need for further research in this area.

Cite This Article

APA
Borunova SF, Tkachev N, Iolchiev B, Artyushina Z, Abramov P, Nikitina M, Silanteva A, Khusnetdinova N, Serezhenkov V. (2020). Estimation of Nitrite-Nitric Oxide Derivative-In Horses with Intestinal Colic by ESR Spectroscopy. Vet Sci, 7(4), 191. https://doi.org/10.3390/vetsci7040191

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 7
Issue: 4
PII: 191

Researcher Affiliations

Borunova, Seid-Fatima
  • The Russian State Center for Animal Feed and Drug Standardization and Quality (VGNKI), Zvenigorodskoye Shosse 5, 123022 Moscow, Russia.
Tkachev, Nikolay
  • Semenov Institute of Chemical Physics, Russian Academy of Sciences, 4 Kosygina Street, Building 1, 119991 Moscow, Russia.
Iolchiev, Baylar
  • L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Russia.
Artyushina, Zinaida
  • Skryabin, Academy of Veterinary Medicine and Biotechnology, 23, Scryabin Str., 109472 Moscow, Russia.
Abramov, Pavel
  • Skryabin, Academy of Veterinary Medicine and Biotechnology, 23, Scryabin Str., 109472 Moscow, Russia.
Nikitina, Marina
  • V.M. Gorbatov Federal Research Center for Food Systems of RAS, 26, Talalikhina Str., 109316 Moscow, Russia.
Silanteva, Anastasia
  • L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, Russia.
Khusnetdinova, Neilia
  • Skryabin, Academy of Veterinary Medicine and Biotechnology, 23, Scryabin Str., 109472 Moscow, Russia.
Serezhenkov, Vladimir
  • Semenov Institute of Chemical Physics, Russian Academy of Sciences, 4 Kosygina Street, Building 1, 119991 Moscow, Russia.

Conflict of Interest Statement

The authors declared that there is no conflict of interests. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Citations

This article has been cited 2 times.
  1. Mirzaei A, Hajimohammadi A. Diagnostic performance of specific oxidative stress biomarkers, acute phase proteins, and certain trace elements in different severities of equine colic. J Equine Sci 2025 Jun;36(2):45-54.
    doi: 10.1294/jes.36.45pubmed: 40832000google scholar: lookup
  2. Hajimohammadi A, Ghane M, Ghari Tehrani M, Paravar B, Mirzaei A, Razavi S, Nikzad M. Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements. J Equine Sci 2023 Sep;34(3):73-81.
    doi: 10.1294/jes.34.73pubmed: 37781567google scholar: lookup
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