Evaluation of refractometry methods for estimating passive immunity status in neonatal foals.
Abstract: The objective of this cross-sectional study was to determine the accuracy of the digital Brix and serum total protein (TP) refractometers for estimating different passive immunity status in neonatal foals. In total, 18- to 40-h old purebred Arabian foals (n = 185) were used. Serum TP concentrations, total solid percentages and IgG concentrations were measured with a digital serum TP refractometer, digital Brix refractometer and the gold standard radial immunodiffusion (RID) assay, respectively. Correlation coefficients were calculated between the refractometer and RID assay results. A receiver operating characteristic analysis was used to select the optimal cut-offs for both refractometers. Test performance and agreement were evaluated using diagnostic test characteristics at optimal thresholds and areas under the ROC curve, and by calculating Cohen's kappa coefficient. The sensitivity and specificity of the digital Brix refractometer at optimal cut-offs (≤7.8%, ≤7.9%, ≤8.2%, ≤8.3%, ≤9.0%) were 100 and 69.3%; 100% and 68.5%; 70.5% and 71.0%; 88.3% and 85.5%; 88.1% and 76% to estimate RID-IgG of <400 mg/dL, <800 mg/dL, <1500 mg/dL, <2500 mg/dL and < 3000 mg/dL, respectively. The sensitivity and specificity of the digital serum TP refractometer at optimal cut-off (≤4.6 g/dL, ≤4.6 g/dL, ≤4.8 g/dL, ≤5.0 g/dL, ≤5.4 g/dL) were 100 and 69.3%; 100% and 72.8%; 90% and 72.8%; 72.9% and 83.9%; 84.4% and 88% to estimate RID-IgG of <400 mg/dL, <800 mg/dL, <1500 mg/dL, <2500 mg/dL and < 3000 mg/dL, respectively. In conclusion, the refractometers showed a good potential as screening tools for the estimation of different IgG concentrations in neonatal foals.
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Publication Date: 2022-09-17 PubMed ID: 36126780DOI: 10.1016/j.jim.2022.113359Google Scholar: Lookup
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- Journal Article
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Summary
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This study evaluates the precision of digital Brix and serum total protein refractometers in assessing the passive immunity status in newborn foal horses. The conclusions suggest that these refractometers demonstrate significant promise as screening tools for estimating different concentrations of the antibody IgG in foals.
Objectives and Methodology
- The researchers performed a cross-sectional study with the primary objective to assess the accuracy of digital Brix and serum total protein refractometers in estimating passive immunity status in neonatal foals.
- The study sample comprised 185 purebred Arabian foals, aged between 18 to 40 hours.
- Serum total protein (TP) concentrations, total solid percentages, and IgG concentrations were measured using digital serum TP and Brix refractometers and standard radial immunodiffusion (RID) assays.
- Correlation coefficients were then calculated between the results from the refractometers and the RID tests.
- Moreover, receiver operating characteristic analysis was used to select optimal cut-offs for both refractometers.
- The test performance and agreement were assessed using diagnostic test characteristics at the optimal thresholds, areas under the ROC curve, and calculated Cohen’s Kappa coefficient.
Results
- The study found that the sensitivity and specificity of the digital Brix refractometer at optimal cut-offs were to estimate RID-IgG ranged from 70.5% to 100% for sensitivity and 68.5% to 85.5% for specificity.
- Similarly, the sensitivity and specificity of the digital serum TP refractometer at optimal cut-off to estimate RID-IgG ranged from 72.9% to 100% for sensitivity and 69.3% to 88% for specificity.
Conclusion
- Based on the results, the researchers concluded that digital Brix and serum total protein refractometers showed good potential as screening tools for estimating different concentrations of IgG in neonatal foals.
- This finding is significant as it enables accurate assessment of a vital component of the immune system, thus aiding in ensuring the health and well-being of newborn foals.
Cite This Article
APA
Akköse M, Karabulut E, Yılmaz İÇ, Dik Ç, İnal Ş, Özbeyaz C, Çam M, Çınar EM, Orakçı D, Durmaz M.
(2022).
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals.
J Immunol Methods, 510, 113359.
https://doi.org/10.1016/j.jim.2022.113359 Publication
Researcher Affiliations
- Department of Livestock, Dalaman Agricultural Enterprise, General Directorate of Agricultural Enterprises, Dalaman, Muğla 48700, Türkiye. Electronic address: akkosem2012@gmail.com.
- Department of Horse Breeding, Anadolu Agricultural Enterprise, General Directorate of Agricultural Enterprises, Mahmudiye, Eskişehir 26800, Türkiye.
- Department of Horse Breeding, Sultansuyu Agricultural Enterprise, General Directorate of Agricultural Enterprises, Akçadağ, Malatya 44600, Türkiye.
- Department of Horse Breeding, Anadolu Agricultural Enterprise, General Directorate of Agricultural Enterprises, Mahmudiye, Eskişehir 26800, Türkiye.
- Department of Animal Sciences, Faculty of Veterinary Medicine, Selcuk University, Selçuklu, Konya 42130, Türkiye.
- Department of Animal Sciences, Faculty of Veterinary Medicine, Ankara University, Dışkapı, Ankara 06110, Türkiye.
- Department of Animal Sciences, Faculty of Veterinary Medicine, Selcuk University, Selçuklu, Konya 42130, Türkiye.
- Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dicle University, Sur, Diyarbakır 21000, Türkiye.
- Laboratory of Progeny Testing, Sultansuyu Agricultural Enterprise, General Directorate of Agricultural Enterprises, Akçadağ, Malatya 44600, Türkiye.
- Department of Horse Breeding, General Directorate of Agricultural Enterprises, Bakanlıklar, Ankara 06530, Türkiye.
MeSH Terms
- Animals
- Animals, Newborn
- Cross-Sectional Studies
- Horses
- Immunodiffusion / methods
- Immunodiffusion / veterinary
- Immunoglobulin G
- Refractometry / methods
- Refractometry / veterinary
- Sensitivity and Specificity
Citations
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