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Parasites & vectors2020; 13(1); 459; doi: 10.1186/s13071-020-04338-1

First report of genetic diversity and risk factor analysis of equine piroplasm infection in equids in Jilin, China.

Abstract: Equine piroplasmosis (EP) is a tick-borne hemoprotozoan disease of equids, caused by Theileria equi and Babesia caballi. Equine piroplasmosis represents a serious challenge to the equine industry due to important economic losses worldwide. The present study aimed to evaluate the prevalence of Theileria equi and Babesia caballi infections in equids from Jilin Province, China. Methods: A total of 220 blood samples (192 horses and 28 donkeys/mules) were collected from March 2018 to October 2019 in five districts of Jilin Province and analyzed by PCR. Potential risk factors, including the region, sex, management, and host species of the animals were assessed in relation to T. equi infection. Moreover, the V4 hypervariable region of the T. equi 18S rRNA gene was analyzed to identify specific genotypes. Results: The overall prevalence of T. equi in equids was 27.7%, whereas B. caballi infection was not identified. The district with the highest positive rate was Baicheng (43.3%), followed by Tonghua (28.9%), Yanbian (26.4%), Jilin (23.3%), and Liaoyuan (20.9%). The sex of the animals and farm management were identified as main risk factors, which were significantly associated with the prevalence of Equine piroplasmosis (P < 0.05). The risk factor analysis indicated that the females were at a higher risk (OR: 2.48, 95% CI: 1.17-5.27) of being infected compared to the males, whereas the organized farm was protective factor (OR: 0.42, 95% CI: 0.22-0.80). The phylogenetic analyses revealed that there were two T. equi genotypes (A and E) in Jilin. Conclusions: Our findings provided important epidemiological data for the prevention and control of T. equi infection in Jilin, China.
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Publication Date: 2020-09-09 PubMed ID: 32907616PubMed Central: PMC7479743DOI: 10.1186/s13071-020-04338-1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses an investigation into the prevalence and genetic diversity of Equine piroplasmosis in horses and donkeys in the Jilin Province, China, highlighting risk factors and suggesting preventive measures.

Objective of the study

  • The primary aim of the research was to evaluate the presence of Theileria equi and Babesia caballi infections, responsible for Equine piroplasmosis, in equids (horses and donkeys) in Jilin Province, China.
  • The study further sought to determine potential risk factors associated with T. equi infection and to analyze the genetic diversity of the parasite in the region.

Methodology

  • The researchers collected blood samples from 192 horses and 28 donkeys/mules across five districts of Jilin Province from March 2018 to October 2019.
  • They used the PCR (Polymerase Chain Reaction) method to analyze the samples for T. equi and B. caballi infections.
  • They considered various risk factors, such as region, sex, management practices, and host species, in relation to T. equi infection.
  • The team also conducted a genetic analysis of the V4 hypervariable region of the T. equi 18S rRNA gene to identify specific genotypes of the parasite present.

Results

  • The overall prevalence of T. equi in equids was found to be 27.7%, whereas no B. caballi infection was detected.
  • District-wise, the infection was most prevalent in Baicheng (43.3%), followed by Tonghua, Yanbian, Jilin, and Liaoyuan.
  • Both the sex of the equids and farm management practices emerged as significant risk factors associated with the prevalence of Equine piroplasmosis.
  • The study found female equids had a higher infection risk, whereas organized farm management acted as a protective factor reducing the prevalence.
  • Genetic analysis showed the presence of two T. equi genotypes (A and E) in Jilin.

Conclusion

  • The findings contribute valuable epidemiological data that can aid in developing strategies to prevent and control T. equi infection in Jilin, China.
  • The research emphasizes the need for vigilant monitoring and effective management practices, especially in regions showing higher infection rates.
  • Further, understanding the genetic diversity of T. equi can help in studying the disease’s evolution and develop targeted treatments.

Cite This Article

APA
Zhao S, Wang H, Zhang S, Xie S, Li H, Zhang X, Jia L. (2020). First report of genetic diversity and risk factor analysis of equine piroplasm infection in equids in Jilin, China. Parasit Vectors, 13(1), 459. https://doi.org/10.1186/s13071-020-04338-1

Publication

Parasites & vectors
ISSN: 1756-3305
NlmUniqueID: 101462774
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 459
PII: 459

Researcher Affiliations

Zhao, Shaowei
  • Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No. 977 Park Road, Yanji, 133000, China.
Wang, Hao
  • Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No. 977 Park Road, Yanji, 133000, China.
Zhang, Shuang
  • Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No. 977 Park Road, Yanji, 133000, China.
Xie, Suzhu
  • Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No. 977 Park Road, Yanji, 133000, China.
Li, Hang
  • Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No. 977 Park Road, Yanji, 133000, China.
Zhang, Xuancheng
  • Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No. 977 Park Road, Yanji, 133000, China.
Jia, Lijun
  • Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Agriculture College of Yanbian University, No. 977 Park Road, Yanji, 133000, China. lijunjia1015@sohu.com.

MeSH Terms

  • Animals
  • Babesia / genetics
  • Babesia / isolation & purification
  • Babesiosis / epidemiology
  • Cattle / parasitology
  • China / epidemiology
  • Equidae / parasitology
  • Genes, Protozoan
  • Genetic Variation
  • Horse Diseases / epidemiology
  • Horses / parasitology
  • Phylogeny
  • Prevalence
  • RNA, Ribosomal, 18S / genetics
  • Risk Factors
  • Sex Factors
  • Theileria / genetics
  • Theileria / isolation & purification
  • Theileriasis / epidemiology
  • Tick-Borne Diseases / veterinary

Conflict of Interest Statement

The authors declare that they have no competing interests.

References

This article includes 36 references
  1. Wise LN, Kappmeyer LS, Mealey RH, Knowles DP. Review of equine piroplasmosis.. J Vet Intern Med 2013 Nov-Dec;27(6):1334-46.
    doi: 10.1111/jvim.12168pubmed: 24033559google scholar: lookup
  2. Jongejan F, Uilenberg G. The global importance of ticks.. Parasitology 2004;129 Suppl:S3-14.
    doi: 10.1017/S0031182004005967pubmed: 15938502google scholar: lookup
  3. Kappmeyer LS, Thiagarajan M, Herndon DR, Ramsay JD, Caler E, Djikeng A, Gillespie JJ, Lau AO, Roalson EH, Silva JC, Silva MG, Suarez CE, Ueti MW, Nene VM, Mealey RH, Knowles DP, Brayton KA. Comparative genomic analysis and phylogenetic position of Theileria equi.. BMC Genomics 2012 Nov 9;13:603.
    doi: 10.1186/1471-2164-13-603pmc: PMC3505731pubmed: 23137308google scholar: lookup
  4. Schein FB, Maia MO, Witter R, Marcili A, Camargo LM, Dutra V, Nakazato L, Candido SL, Almeida EM, Oliveira ACS, Pacheco RC. Molecular survey and genetic diversity of piroplasmids in equids from Midwestern Brazil.. Rev Bras Parasitol Vet 2018 Oct-Dec;27(4):464-472.
    doi: 10.1590/s1984-296120180048pubmed: 30183997google scholar: lookup
  5. Hou HB, Li HJ, Zhang L. Research advences on functional genes of main economic traits in horses and donkeys.. Chin An Husb Vet Med 2018;45:2670–2680.
  6. Wang M, Guo W, Igarashi I, Xuan X, Wang X, Xiang W, Jia H. Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays.. J Vet Med Sci 2014 Apr;76(4):549-52.
    doi: 10.1292/jvms.13-0477pmc: PMC4064140pubmed: 24292247google scholar: lookup
  7. Xue Y. Serological and pathogenic study of the major infectious diseases in Guizhou pony.. Ph.D. Thesis, Guizhou University, Guiyang; 2016.
  8. Zhang Y, Chahan B, Liu S, Song R, Li Y, Huercha, Guo Q, Wu H, Zhu Y. Epidemiologic studies on Theileria equi infections for grazing horses in Ili of Xinjiang province.. Vet Parasitol 2017 Sep 15;244:111-113.
    doi: 10.1016/j.vetpar.2017.07.014pubmed: 28917300google scholar: lookup
  9. Battsetseg B, Lucero S, Xuan X, Claveria FG, Inoue N, Alhassan A, Kanno T, Igarashi I, Nagasawa H, Mikami T, Fujisaki K. Detection of natural infection of Boophilus microplus with Babesia equi and Babesia caballi in Brazilian horses using nested polymerase chain reaction.. Vet Parasitol 2002 Aug 22;107(4):351-7.
    doi: 10.1016/S0304-4017(02)00131-0pubmed: 12163246google scholar: lookup
  10. Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi I. Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood.. Vet Parasitol 2005 Apr 20;129(1-2):43-9.
    doi: 10.1016/j.vetpar.2004.12.018pubmed: 15817201google scholar: lookup
  11. Huang YH, He GQ, Peng DP, Li WY, Zhang XP. Serological investigation for equine piroplasmosis in region of Dongwan, Guangdong.. Heilongjiang Anim Sci Vet Med 2016;8:146–147.
  12. Ning K, Li KH, Yang XC, Wang J. Seroprevalence of equine piroplasmosis and trypanosomiasis in horses in Shanghai.. Shanghai J Anim Husb Vet Med 2014;5:38–39.
  13. Wang J, Liu J, Yang J, Wang X, Li Z, Jianlin X, Li X, Xiang Q, Li Y, Liu Z, Luo J, Guan G, Yin H. The first molecular detection and genetic diversity of Babesia caballi and Theileria equi in horses of Gansu province, China.. Ticks Tick Borne Dis 2019 Apr;10(3):528-532.
    doi: 10.1016/j.ttbdis.2019.01.003pubmed: 30670354google scholar: lookup
  14. Xiong HZ, Liu B, Zhang SF. Establishment and application of the PCR method for detection of Babesia equi.. Agric Sci J Yanbian Univ 2007;29:129–133.
  15. Battsetseg B, Xuan X, Ikadai H, Bautista JL, Byambaa B, Boldbaatar D, Battur B, Battsetseg G, Batsukh Z, Igarashi I, Nagasawa H, Mikami T, Fujisaki K. Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks.. Int J Parasitol 2001 Apr;31(4):384-6.
    doi: 10.1016/S0020-7519(01)00120-5pubmed: 11306116google scholar: lookup
  16. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.. Nucleic Acids Res 1994 Nov 11;22(22):4673-80.
    doi: 10.1093/nar/22.22.4673pmc: PMC308517pubmed: 7984417google scholar: lookup
  17. Sun Z, Jiang C, Feng J, Yang W, Li M, Miao W. Phylogenomic analysis of Balantidium ctenopharyngodoni (Ciliophora, Litostomatea) based on single-cell transcriptome sequencing.. Parasite 2017;24:43.
    doi: 10.1051/parasite/2017043pmc: PMC5684829pubmed: 29134943google scholar: lookup
  18. Bartolomé Del Pino LE, Nardini R, Veneziano V, Iacoponi F, Cersini A, Autorino GL, Buono F, Scicluna M. Babesia caballi and Theileria equi infections in horses in Central-Southern Italy: Sero-molecular survey and associated risk factors.. Ticks Tick Borne Dis 2016 Apr;7(3):462-9.
    doi: 10.1016/j.ttbdis.2016.01.011pubmed: 26847198google scholar: lookup
  19. Urdaz-Rodríguez JH, Fosgate GT, Alleman AR, Rae DO, Donovan GA, Melendez P. Seroprevalence estimation and management factors associated with high herd seropositivity for Anaplasma marginale in commercial dairy farms of Puerto Rico.. Trop Anim Health Prod 2009 Oct;41(7):1439-48.
    doi: 10.1007/s11250-009-9332-9pubmed: 19337849google scholar: lookup
  20. Dahiya R, Salar RK, Mandal KD, Kumar R, Tripathi BN, Pal Y, Kumar S. Risk factor analysis associated with Theileria equi infected equines in semi-arid and sub-humid ecological enzootic zones of India.. Vet Parasitol Reg Stud Reports 2018 May;12:17-21.
    pubmed: 31014801doi: 10.1016/j.vprsr.2018.01.005google scholar: lookup
  21. Liu Q, Meli ML, Zhang Y, Meili T, Stirn M, Riond B, Weibel B, Hofmann-Lehmann R. Sequence heterogeneity in the 18S rRNA gene in Theileria equi from horses presented in Switzerland.. Vet Parasitol 2016 May 15;221:24-9.
    doi: 10.1016/j.vetpar.2016.03.003pubmed: 27084467google scholar: lookup
  22. Xiong HZ, Zhang SF, Liu B. An investigation of Babesia equi in horses in Jilin Province.. Chin J Vet Med 2009;45:40–41.
  23. García-Bocanegra I, Arenas-Montes A, Hernández E, Adaszek L, Carbonero A, Almería S, Jaén-Téllez JA, Gutiérrez-Palomino P, Arenas A. Seroprevalence and risk factors associated with Babesia caballi and Theileria equi infection in equids.. Vet J 2013 Feb;195(2):172-8.
    doi: 10.1016/j.tvjl.2012.06.012pubmed: 22784418google scholar: lookup
  24. Qablan MA, Oborník M, Petrželková KJ, Sloboda M, Shudiefat MF, Hořín P, Lukeš J, Modrý D. Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity.. Parasitology 2013 Aug;140(9):1096-103.
    doi: 10.1017/S0031182013000486pubmed: 23673249google scholar: lookup
  25. Hussain MH, Saqib M, Raza F, Muhammad G, Asi MN, Mansoor MK, Saleem M, Jabbar A. Seroprevalence of Babesia caballi and Theileria equi in five draught equine populated metropolises of Punjab, Pakistan.. Vet Parasitol 2014 May 28;202(3-4):248-56.
    doi: 10.1016/j.vetpar.2014.01.026pubmed: 24582524google scholar: lookup
  26. de Waal DT. Equine piroplasmosis: a review.. Br Vet J 1992 Jan-Feb;148(1):6-14.
    doi: 10.1016/0007-1935(92)90061-5pubmed: 1551016google scholar: lookup
  27. Ueti MW, Palmer GH, Scoles GA, Kappmeyer LS, Knowles DP. Persistently infected horses are reservoirs for intrastadial tick-borne transmission of the apicomplexan parasite Babesia equi.. Infect Immun 2008 Aug;76(8):3525-9.
    doi: 10.1128/IAI.00251-08pmc: PMC2493223pubmed: 18490466google scholar: lookup
  28. Heuchert CM, de Giulli V Jr, de Athaide DF, Böse R, Friedhoff KT. Seroepidemiologic studies on Babesia equi and Babesia caballi infections in Brazil.. Vet Parasitol 1999 Aug 16;85(1):1-11.
    doi: 10.1016/S0304-4017(99)00108-9pubmed: 10447188google scholar: lookup
  29. Abutarbush SM, Alqawasmeh DM, Mukbel RM, Al-Majali AM. Equine babesiosis: seroprevalence, risk factors and comparison of different diagnostic methods in Jordan.. Transbound Emerg Dis 2012 Feb;59(1):72-8.
    doi: 10.1111/j.1865-1682.2011.01244.xpubmed: 21771287google scholar: lookup
  30. Sumbria D, Singla LD, Sharma A, Bal MS, Randhawa CS. Molecular survey in relation to risk factors and haemato-biochemical alteration in Theileria equi infection of equines in Punjab Province, India.. Vet Parasitol Reg Stud Reports 2017 May;8:43-50.
    pubmed: 31014636doi: 10.1016/j.vprsr.2017.01.009google scholar: lookup
  31. Dos Santos TM, Roier EC, Santos HA, Pires MS, Vilela JA, Moraes LM, Almeida FQ, Baldani CD, Machado RZ, Massard CL. Factors associated to Theileria equi in equids of two microregions from Rio de Janeiro, Brazil.. Rev Bras Parasitol Vet 2011 Jul-Sep;20(3):235-41.
    doi: 10.1590/S1984-29612011000300011pubmed: 21961755google scholar: lookup
  32. Roberts CW, Walker W, Alexander J. Sex-associated hormones and immunity to protozoan parasites.. Clin Microbiol Rev 2001 Jul;14(3):476-88.
    doi: 10.1128/CMR.14.3.476-488.2001pmc: PMC88985pubmed: 11432809google scholar: lookup
  33. Sumbria D, Singla LD, Kaur P. Sero-prevalence and risk factor analysis of Theileria equi infection in equids from different agro-climatic zones of Punjab (India) by Indirect Immunofluorescence Antibody test.. Vet Parasitol Reg Stud Reports 2018 Aug;13:18-20.
    pubmed: 31014870doi: 10.1016/j.vprsr.2018.03.003google scholar: lookup
  34. Piantedosi D, D'Alessio N, Di Loria A, Di Prisco F, Mariani U, Neola B, Santoro M, Montagnaro S, Capelli G, Veneziano V. Seroprevalence and risk factors associated with Babesia caballi and Theileria equi infections in donkeys from Southern Italy.. Vet J 2014 Dec;202(3):578-82.
    doi: 10.1016/j.tvjl.2014.09.025pubmed: 25457263google scholar: lookup
  35. Kouam MK, Kantzoura V, Masuoka PM, Gajadhar AA, Theodoropoulos G. Genetic diversity of equine piroplasms in Greece with a note on speciation within Theileria genotypes (T. equi and T. equi-like).. Infect Genet Evol 2010 Oct;10(7):963-8.
    doi: 10.1016/j.meegid.2010.06.008pubmed: 20601168google scholar: lookup
  36. Nagore D, García-Sanmartín J, García-Pérez AL, Juste RA, Hurtado A. Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis.. Vet Parasitol 2004 Aug 13;123(1-2):41-54.
    doi: 10.1016/j.vetpar.2004.04.010pubmed: 15265570google scholar: lookup

Citations

This article has been cited 9 times.
  1. Liang W, Zhao S, Wang N, Tang Z, Zhao F, Liu M, Jin W, Meng Y, Jia L. Molecular occurrence and risk factors for Toxoplasma gondii infection in equids in Jilin, China. Sci Rep 2022 Jul 30;12(1):13121.
    doi: 10.1038/s41598-022-16658-6pubmed: 35907906google scholar: lookup
  2. Elsawy BSM, Nassar AM, Alzan HF, Bhoora RV, Ozubek S, Mahmoud MS, Kandil OM, Mahdy OA. Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of Theileria haneyi in Egypt. Pathogens 2021 Oct 31;10(11).
    doi: 10.3390/pathogens10111414pubmed: 34832570google scholar: lookup
  3. Galon EM, Macalanda AM, Garcia MM, Ibasco CJ, Garvida A, Ji S, Zafar I, Hasegawa Y, Liu M, Ybañez RH, Umemiya-Shirafuji R, Ybañez A, Claveria F, Xuan X. Molecular Identification of Selected Tick-Borne Protozoan and Bacterial Pathogens in Thoroughbred Racehorses in Cavite, Philippines. Pathogens 2021 Oct 13;10(10).
    doi: 10.3390/pathogens10101318pubmed: 34684266google scholar: lookup
  4. Dao TTH, Szűts T, Duong NN, Troung DTQ, Solymosi N, Takács N, Hornok S, Farkas R. The first molecular detection of equine piroplasmosis in Vietnam and genetic characterization of three co-circulating genotypes of Theileria equi. Parasitol Res 2026 Feb 5;125(1):14.
    doi: 10.1007/s00436-026-08630-4pubmed: 41644779google scholar: lookup
  5. Qin S, Kulabieke T, Mizhamuhan D, Zhang M, Jin M, Abula G, Pi M, Wang H, Zhang Y, Guo Q. Molecular Prevalence and Genotypic Diversity of Theileria equi in Xinjiang, China, Based on Three Genes. Vet Sci 2025 Dec 25;13(1).
    doi: 10.3390/vetsci13010027pubmed: 41600683google scholar: lookup
  6. Khan MZ, Li Y, Zhu M, Li M, Wang T, Zhang Z, Liu W, Ma Q, Wang C. Advances in Donkey Disease Surveillance and Microbiome Characterization in China. Microorganisms 2025 Mar 26;13(4).
    doi: 10.3390/microorganisms13040749pubmed: 40284586google scholar: lookup
  7. Sadeddine R, Righi S, Saidani K, Benakhla A. First Molecular Characterization of Theileria equi from Northeastern Algeria. Acta Parasitol 2025 Mar 6;70(2):66.
    doi: 10.1007/s11686-025-01006-1pubmed: 40050535google scholar: lookup
  8. Min P, Song J, Zhao S, Ma Z, Meng Y, Tang Z, Wang Z, Lin S, Zhao F, Liu M, Wang L, Jia L. Tick species, tick-borne pathogen distribution and risk factor analysis in border areas of China, Russia and North Korea. Front Vet Sci 2025;12:1529253.
    doi: 10.3389/fvets.2025.1529253pubmed: 40007747google scholar: lookup
  9. Cui Y, Cao M, Yu F, Zhao A, Tao D, Zhu T, Zhang Z, Qi M. Molecular detection of piroplasms in domestic donkeys in Xinjiang, China. Vet Med Sci 2024 Jul;10(4):e1468.
    doi: 10.1002/vms3.1468pubmed: 38879882google scholar: lookup
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