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Scientific reports2017; 7(1); 696; doi: 10.1038/s41598-017-00803-7

From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species.

Abstract: Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells. We further characterized the cells and identified their clonogenic and immunomodulatory capacities, their immune-phenotyping behavior and their capability to differentiate into chondroblasts, osteoblasts and adipocytes. In conclusion, this report describes a novel and easy-to-use technique of skeletal muscle-derived mesenchymal stem cell harvest, culture, characterization. This technique is transposable to a multitude of different animal species.
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Publication Date: 2017-04-06 PubMed ID: 28386120PubMed Central: PMC5429713DOI: 10.1038/s41598-017-00803-7Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a novel and minimally invasive technique for obtaining adult mesenchymal stem cells from skeletal muscle, instead of the traditional sources – bone marrow and adipose tissue. This method applies to multiple species such as humans, pigs, dogs and horses, and the harvested cells are capable of differentiating into several cell types.

Introduction

  • The study presents a new way of extracting adult mesenchymal stem cells, which are cells that can differentiate into a variety of cell types. Instead of utilising bone marrow and fat tissue, which are commonly used but difficult to harvest and do not always yield sufficient cells, this research proposes using skeletal muscle tissue.

Methodology

  • Microbiopsies of skeletal muscle in man, pigs, dogs and horses were taken for the study. This sampling method is easier, less invasive and more efficient than traditional extraction from bone marrow and adipose (fat) tissue. This ensures that sufficient cell numbers can be obtained without resorting to invasive procedures.
  • The process involves explant of the sample and using a Percoll density gradient for isolation, followed by growing the cells in a cultured environment. Percoll density gradient is a type of cell isolation method that separates cells based on their size and density.

Characterization of Cells

  • The extracted mesenchymal stem cells were further examined for their clonogenic (ability to produce clones) and immunomodulatory capacities (capacity of the cells to modulate or regulate the functioning of the immune system).
  • The immune-phenotyping behavior, i.e., the phenotypic properties of the cells that affect their recognition by the immune system, was also studied.
  • Furthermore, these cells were analyzed for their potential to differentiate into chondroblasts (cells that form cartilage), osteoblasts (cells that form bone) and adipocytes (fat cells).

Conclusion

  • In summary, this study introduces a new, less invasive technique to harvest and culture skeletal muscle-derived mesenchymal stem cells. The novelty also lies in its adaptability to multiple species including humans, pigs, dogs, and horses. The harvested cells show potential in differentiating into several cell types, thereby, offering broad applications in the medical and veterinary field.

Cite This Article

APA
Ceusters J, Lejeune JP, Sandersen C, Niesten A, Lagneaux L, Serteyn D. (2017). From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species. Sci Rep, 7(1), 696. https://doi.org/10.1038/s41598-017-00803-7

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 7
Issue: 1
Pages: 696
PII: 696

Researcher Affiliations

Ceusters, J
  • Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000, Liège, Belgium. j.ceusters@ulg.ac.be.
Lejeune, J-Ph
  • Mont Le Soie Equine Research Center, Mont Le Soie, 1 6690, Vielsalm, Belgium.
Sandersen, C
  • Department of Clinical Sciences, Equine Surgery, Faculty of Veterinary Medicine B41, University of Liège, Sart Tilman, 4000, Liège, Belgium.
Niesten, A
  • Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000, Liège, Belgium.
Lagneaux, L
  • Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Université Libre de Bruxelles, Campus Erasme, Brussels, Belgium.
Serteyn, D
  • Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000, Liège, Belgium.
  • Mont Le Soie Equine Research Center, Mont Le Soie, 1 6690, Vielsalm, Belgium.
  • Department of Clinical Sciences, Equine Surgery, Faculty of Veterinary Medicine B41, University of Liège, Sart Tilman, 4000, Liège, Belgium.

MeSH Terms

  • Adult Stem Cells / cytology
  • Adult Stem Cells / metabolism
  • Animals
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • Cell Differentiation
  • Cells, Cultured
  • Dogs
  • Horses
  • Humans
  • Immunomodulation
  • Immunophenotyping
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Muscle, Skeletal / cytology
  • Phenotype
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Swine

Conflict of Interest Statement

The method described in this manuscript is patented: Mammalian muscle-derived stem cells, WO2015091210. J. Ceusters and D. Serteyn are co-inventors on this patent. The remaining authors declare no competing financial interest.

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