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Home / Equine Research Bank / Reprod Domest Anim / Glass wool column filtration for stallion sperm selection: A...
Reproduction in domestic animals = Zuchthygiene2023; 58(9); 1244-1250; doi: 10.1111/rda.14424

Glass wool column filtration for stallion sperm selection: A comparative analysis with the single-layer colloid centrifugation.

Abstract: Glass wool column filtration (GWCF) selects human, bull, boar, dog and buffalo spermatozoa, but reports in the horse are scarce. Single-layer colloid centrifugation with Androcoll-E™ is currently the standard procedure to select good-quality equine sperm. This study was designed to assess GWCF (50 and 75 mg columns; GWCF-50 and GWCF-75, respectively) efficacy to select good-quality sperm from fresh and frozen-thawed equine semen, and to compare its performance with Androcoll-E™ colloid centrifugation. Percentage total motile (TM), progressively motile (PM), morphologically normal (MN), osmotically competent (HOS+) and acrosome-intact/osmotically competent (AI/HOS+) sperm were determined. In studies done with fresh semen samples (n = 17), suspensions subjected to GWCF-50 showed an improvement (p < .05) in PM and HOS+ sperm after selection. With GWCF-75, an increase (p < .05) in PM, MN and HOS+ sperm was observed. Results with GWCF were comparable or better than with Androcoll-E™ selection. Sperm recovery was similar between procedures for all semen parameters. Total sperm count recovery was lower after GWCF-75 (GWCF-50 = 60.0; GWCF-75 = 51.0; Androcoll-E™ = 76.0 million sperm; median; p = .013), but results on total progressive sperm count were similar (GWCF-50 = 23.0; GWCF-75 = 27.0; Androcoll-E™ = 24.0 million sperm; median; p = .3850). Using frozen-thawed semen samples (n = 16), an improvement (p < .05) in TM, PM, NM, HOS+ and AI/HOS+ sperm was observed in GWCF-75 filtrates. Results were comparable to Androcoll-E™ centrifugation, except HOS+ that increased (p < .05) only after GWCF-75. Recovery was comparable for all parameters in frozen samples. GWCF is a simple and low-cost procedure that selects equine sperm with a quality comparable to colloid centrifugation with Androcoll-E™.
© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
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Publication Date: 2023-07-19 PubMed ID: 37402154DOI: 10.1111/rda.14424Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study comparing the effectiveness of glass wool column filtration (GWCF) and Androcoll-E™ colloid centrifugation in selecting high-quality sperm from stallions. The main finding is that GWCF, a simpler and less expensive procedure, performs comparably, and in some cases better than the standard centrifugation method.

Objective of the Study

  • The study aimed to evaluate the efficacy of GWCF, specifically using 50mg and 75mg columns (designated as GWCF-50 and GWCF-75), in selecting high-quality sperm from both fresh and frozen-thawed equine semen.
  • It also sought to compare the performance of GWCF with the currently accepted method of sperm selection in horses, which is single-layer Androcoll-E™ colloid centrifugation.

Methodology and Parameters

  • The researchers examined various parameters of sperm quality, including the percentage of total motile (TM), progressively motile (PM), morphologically normal (MN), osmotically competent (HOS+), and acrosome-intact/osmotically competent (AI/HOS+) sperm.
  • The study was conducted using fresh semen samples (n = 17) and frozen-thawed semen samples (n = 16).

Findings

  • The results showed an improvement in the PM and HOS+ parameters in samples subjected to GWCF-50, while GWCF-75 led to an increase in PM, MN and HOS+ parameters.
  • The performance of GWCF in selecting high-quality sperm was found to be comparable or better than that of Androcoll-E™ centrifugation.
  • The total sperm count recovery was lower after GWCF-75, but the total progressive sperm count was similar for both GWCF and Androcoll-E™.
  • With frozen-thawed semen samples, an improvement was observed in all sperm parameters with GWCF-75 filtration, and its results were comparable to Androcoll-E™, except for a notable increase in the HOS+ parameter which only occurred with GWCF-75.

Conclusion

  • The research concludes that GWCF is a simpler and cost-effective alternative to Androcoll-E™ centrifugation for selecting high-quality equine sperm.
  • The study suggests that its effectiveness is comparable, and in some regards superior, to the standard centrifugation method, making it a viable alternative for sperm selection in horses.

Cite This Article

APA
Zevallos Valenzuela GE, Ferrante A, Verón GL, Miragaya M, Marín-Briggiler CI, Vazquez-Levin MH. (2023). Glass wool column filtration for stallion sperm selection: A comparative analysis with the single-layer colloid centrifugation. Reprod Domest Anim, 58(9), 1244-1250. https://doi.org/10.1111/rda.14424

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 58
Issue: 9
Pages: 1244-1250

Researcher Affiliations

Zevallos Valenzuela, Giuliana Elizabeth
  • Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET), Fundación IBYME (FIBYME), Buenos Aires, Argentina.
Ferrante, Alejandro
  • Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET), Fundación IBYME (FIBYME), Buenos Aires, Argentina.
  • Cátedra de Teriogenología, UBA, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Buenos Aires, Argentina.
Verón, Gustavo Luis
  • Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET), Fundación IBYME (FIBYME), Buenos Aires, Argentina.
Miragaya, Marcelo
  • Cátedra de Teriogenología, UBA, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Buenos Aires, Argentina.
Marín-Briggiler, Clara Isabel
  • Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET), Fundación IBYME (FIBYME), Buenos Aires, Argentina.
Vazquez-Levin, Mónica Hebe
  • Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET), Fundación IBYME (FIBYME), Buenos Aires, Argentina.

MeSH Terms

  • Male
  • Animals
  • Horses
  • Swine
  • Humans
  • Dogs
  • Semen
  • Cryopreservation / veterinary
  • Cryopreservation / methods
  • Spermatozoa
  • Semen Preservation / veterinary
  • Semen Preservation / methods
  • Colloids
  • Centrifugation / veterinary
  • Centrifugation / methods
  • Buffaloes
  • Bison
  • Sperm Motility

Grant Funding

  • CONICET PIP881
  • Fundación René Barón
  • Fundación Williams

References

This article includes 21 references
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Citations

This article has been cited 1 times.
  1. Andrei CR, Posastiuc FP, Constantin NT, Mitrea IL. New insights into semen separation techniques in buffaloes. Front Vet Sci 2023;10:1347482.
    doi: 10.3389/fvets.2023.1347482pubmed: 38269362google scholar: lookup
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