Hemosiderin; isolation from horse spleen and characterization.
Abstract: 1. A method is outlined for isolating hemosiderin from horse spleen by sedimentation from salt solutions of different specific gravities. 2. The iron, nitrogen, phosphorus and ash concentration varied considerably in hemosiderin samples obtained from different spleens. Fractionation of these preparations by sedimentation in organic liquids showed that fractions could be obtained which varied in their iron concentrations from about 25 to 41% iron. 3. Hemosiderin has been found to contain hexoseamine, galactose, mannose and fucose.
Publication Date: 1957-07-01 PubMed ID: 13453489DOI: 10.3181/00379727-95-23272Google Scholar: Lookup
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- Journal Article
Summary
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This research outlines a method for isolating a naturally occurring iron-storage substance, hemosiderin, from horse spleen and provides details regarding its varied composition of iron, nitrogen, phosphorus and ash.
Method of Isolating Hemosiderin
- The researchers have detailed a method of extracting hemosiderin – which is a form of stored iron in the body – from horse spleen.
- This process is accomplished through sedimentation using different salt solutions with varying degrees of specific gravity.
Variations In Chemical Concentration
- It was found that the chemical concentration of iron, nitrogen, phosphorous, and ash significantly varied in hemosiderin samples collected from different spleens.
- By segregating these samples through sedimentation in organic liquids, fractions with different iron concentrations, ranging between 25-41%, were revealed.
Hemosiderin Constituents
- Furthermore, the study found that hemosiderin contains several sugar-type substances: hexoseamine, galactose, mannose, and fucose.
- These components suggest a complex biochemical composition and functional role of hemosiderin in the body, which is important for understanding the body’s iron storage system and could have implications for managing iron-related conditions.
Cite This Article
APA
LUDEWIG S.
(1957).
Hemosiderin; isolation from horse spleen and characterization.
Proc Soc Exp Biol Med, 95(3), 514-517.
https://doi.org/10.3181/00379727-95-23272 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Hematologic Tests
- Hemosiderin
- Horses
- Humans
- Pigments, Biological / analysis
- Spleen
Citations
This article has been cited 6 times.- SCHREIBER M, SANDRITTER W, GEDIGK P. [Ultraviolet microspectrophotometric investigations on iron pigment].. Virchows Arch Pathol Anat Physiol Klin Med 1960;333:288-93.
- DRYSDALE JW, RAMSAY WN. THE SEPARATION OF FERRITIN AND HAEMOSIDERIN FOR STUDIES IN THE METABOLISM OF IRON.. Biochem J 1965 Apr;95(1):282-8.
- RICHTER GW. The nature of storage iron in idiopathic hemochromatosis and in hemosiderosis. Electron optical, chemical, and serologic studies on isolated hemosiderin granules.. J Exp Med 1960 Oct 1;112(4):551-70.
- GOLBERG L, MARTIN LE, BATCHELOR A. Biochemical changes in the tissues of animals injected with iron: acid phosphatase and other enzymes.. Biochem J 1960 Nov;77(2):252-62.
- Lillie RD, Geer JC. On the relation of enterosiderosis pigments of man and guinea pig. Melanosis and pseudomelanosis of colon and villi and the intestinal iron uptake and storage mechanism. Histochemical and experimental studies.. Am J Pathol 1965 Dec;47(6):965-1009.
- Richter GW. The iron-loaded cell--the cytopathology of iron storage. A review.. Am J Pathol 1978 May;91(2):362-404.
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