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Home / Equine Research Bank / J Anim Sci / Hepatocyte growth factor acts as a mitogen for equine satell...
Journal of animal science2018; 96(9); 3645-3656; doi: 10.1093/jas/sky234

Hepatocyte growth factor acts as a mitogen for equine satellite cells via protein kinase C δ-directed signaling.

Abstract: Hepatocyte growth factor (HGF) signals mediate mouse skeletal muscle stem cell, or satellite cell (SC), reentry into the cell cycle and myoblast proliferation. Because the athletic horse experiences exercise-induced muscle damage, the objective of the experiment was to determine the effect of HGF on equine SC (eqSC) bioactivity. Fresh isolates of adult eqSC were incubated with increasing concentrations of HGF and the initial time to DNA synthesis was measured. Media supplementation with HGF did not shorten (P > 0.05) the duration of G0/G1 transition suggesting the growth factor does not affect activation. Treatment with 25 ng/mL HGF increased (P 0.05) on HGF-mediated 5-ethynyl-2'-deoxyuridine (EdU) incorporation. By contrast, treatment of eqSC with 2 µm Gö6983, a pan-protein kinase C (PKC) inhibitor, blocked (P 0.05) HGF-mediated EdU incorporation. The siPKCδ was specific to the kinase and did not affect (P > 0.05) expression of either PKCα or PKCε. Treatment of confluent eqSC with 25 ng/mL HGF suppressed (P < 0.05) nuclear myogenin expression during the early stages of differentiation. These results demonstrate that HGF may not affect activation but can act as a mitogen and modest suppressor of differentiation.
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Publication Date: 2018-06-20 PubMed ID: 29917108PubMed Central: PMC6127786DOI: 10.1093/jas/sky234Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores how Hepatocyte growth factor (HGF) affects the bioactivity of equine satellite cells (eqSC). The findings reveal that HGF does not impact cell activation but can act as a growth stimulant and a slight suppressor of cell differentiation.

Research Overview

  • The study aimed to examine how Hepatocyte growth factor (HGF) influences the bioactivity of equine satellite cells (eqSC), which are vital for muscle repair and regeneration in horses.
  • The researchers conducted experiments on fresh eqSC isolates, which were treated with varying concentrations of HGF, and then the initial time to DNA synthesis was measured.

Key Findings

  • The study discovered that HGF does not shorten the duration of the G0/G1 transition, indicating that this growth factor has no impact on cell activation.
  • However, upon treatment with 25 ng/mL of HGF, eqSC proliferation was observed along with the phosphorylation of ERK1/2 and AKT1, proteins that play significant roles in cell division and survival.
  • Surprisingly, the inhibition of upstream activators of ERK1/2 or AKT1 had no effect on HGF-mediated EdU incorporation, a measure of cell proliferation.
  • On the other hand, the use of a protein kinase C (PKC) inhibitor effectively blocked HGF-induced mitotic activity, indicating an essential role of PKC in the HGF-directed signaling pathway for eqSC proliferation.

Further Insights

  • Gene expression analysis revealed that eqSC express PKCα, PKCδ, and PKCε isoforms.
  • A targeted knockdown of PKCδ with a small interfering RNA (siRNA) effectively prevented HGF-mediated EdU incorporation, further underlining the role of PKC in this process.
  • The siRNA used was specific to PKCδ and had no observable impact on the expression of other PKC isoforms, namely PKCα and PKCε.
  • Finally, it was observed that treatment of eqSC with 25 ng/mL HGF suppressed nuclear myogenin expression during early differentiation stages showing that HGF has a modest inhibitory effect on differentiation processes.

Cite This Article

APA
Brandt AM, Kania JM, Gonzalez ML, Johnson SE. (2018). Hepatocyte growth factor acts as a mitogen for equine satellite cells via protein kinase C δ-directed signaling. J Anim Sci, 96(9), 3645-3656. https://doi.org/10.1093/jas/sky234

Publication

Journal of animal science
ISSN: 1525-3163
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 96
Issue: 9
Pages: 3645-3656

Researcher Affiliations

Brandt, Amanda M
  • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg VA.
Kania, Joanna M
  • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg VA.
Gonzalez, Madison L
  • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg VA.
Johnson, Sally E
  • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg VA.

MeSH Terms

  • Animals
  • Cell Differentiation / physiology
  • Cell Division
  • Hepatocyte Growth Factor / physiology
  • Horses / genetics
  • Horses / metabolism
  • Humans
  • Mitogen-Activated Protein Kinase 3
  • Mitogens
  • Myoblasts
  • Phosphorylation
  • Protein Kinase C-alpha
  • Protein Kinase C-delta / physiology
  • RNA, Small Interfering / metabolism
  • Signal Transduction

References

This article includes 53 references
  1. Allen RE, Sheehan SM, Taylor RG, Kendall TL, Rice GM. Hepatocyte growth factor activates quiescent skeletal muscle satellite cells in vitro.. J Cell Physiol 1995 Nov;165(2):307-12.
    doi: 10.1002/jcp.1041650211pubmed: 7593208google scholar: lookup
  2. Anderson JE. Hepatocyte Growth Factor and Satellite Cell Activation.. Adv Exp Med Biol 2016;900:1-25.
    doi: 10.1007/978-3-319-27511-6_1pubmed: 27003394google scholar: lookup
  3. Barrow-McGee R, Kermorgant S. Met endosomal signalling: in the right place, at the right time.. Int J Biochem Cell Biol 2014 Apr;49:69-74.
    doi: 10.1016/j.biocel.2014.01.009pubmed: 24440758google scholar: lookup
  4. Bi P, Yue F, Sato Y, Wirbisky S, Liu W, Shan T, Wen Y, Zhou D, Freeman J, Kuang S. Stage-specific effects of Notch activation during skeletal myogenesis. Elife 5:3593.
    doi: 10.7554/elife.17355google scholar: lookup
  5. Bjornson CR, Cheung TH, Liu L, Tripathi PV, Steeper KM, Rando TA. Notch signaling is necessary to maintain quiescence in adult muscle stem cells.. Stem Cells 2012 Feb;30(2):232-42.
    doi: 10.1002/stem.773pmc: PMC3384696pubmed: 22045613google scholar: lookup
  6. Bryan K, McGivney BA, Farries G, McGettigan PA, McGivney CL, Gough KF, MacHugh DE, Katz LM, Hill EW. Equine skeletal muscle adaptations to exercise and training: evidence of differential regulation of autophagosomal and mitochondrial components.. BMC Genomics 2017 Aug 9;18(1):595.
    doi: 10.1186/s12864-017-4007-9pmc: PMC5551008pubmed: 28793853google scholar: lookup
  7. Cassano M, Biressi S, Finan A, Benedetti L, Omes C, Boratto R, Martin F, Allegretti M, Broccoli V, Cusella De Angelis G, Comoglio PM, Basilico C, Torrente Y, Michieli P, Cossu G, Sampaolesi M. Magic-factor 1, a partial agonist of Met, induces muscle hypertrophy by protecting myogenic progenitors from apoptosis.. PLoS One 2008 Sep 16;3(9):e3223.
    pmc: PMC2528937pubmed: 18795097doi: 10.1371/journal.pone.0003223google scholar: lookup
  8. Charville GW, Cheung TH, Yoo B, Santos PJ, Lee GK, Shrager JB, Rando TA. Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells.. Stem Cell Reports 2015 Oct 13;5(4):621-32.
    doi: 10.1016/j.stemcr.2015.08.004pmc: PMC4624935pubmed: 26344908google scholar: lookup
  9. Egner IM, Bruusgaard JC, Gundersen K. Satellite cell depletion prevents fiber hypertrophy in skeletal muscle.. Development 2016 Aug 15;143(16):2898-906.
    doi: 10.1242/dev.134411pubmed: 27531949google scholar: lookup
  10. Fukada S, Yamaguchi M, Kokubo H, Ogawa R, Uezumi A, Yoneda T, Matev MM, Motohashi N, Ito T, Zolkiewska A, Johnson RL, Saga Y, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H. Hesr1 and Hesr3 are essential to generate undifferentiated quiescent satellite cells and to maintain satellite cell numbers.. Development 2011 Nov;138(21):4609-19.
    doi: 10.1242/dev.067165pmc: PMC3265560pubmed: 21989910google scholar: lookup
  11. Galimov A, Merry TL, Luca E, Rushing EJ, Mizbani A, Turcekova K, Hartung A, Croce CM, Ristow M, Krützfeldt J. MicroRNA-29a in Adult Muscle Stem Cells Controls Skeletal Muscle Regeneration During Injury and Exercise Downstream of Fibroblast Growth Factor-2.. Stem Cells 2016 Mar;34(3):768-80.
    doi: 10.1002/stem.2281pubmed: 26731484google scholar: lookup
  12. Goh Q, Millay DP. Requirement of myomaker-mediated stem cell fusion for skeletal muscle hypertrophy.. Elife 2017 Feb 10;6.
    pmc: PMC5338923pubmed: 28186492doi: 10.7554/elife.20007google scholar: lookup
  13. Griger J, Schneider R, Lahmann I, Schöwel V, Keller C, Spuler S, Nazare M, Birchmeier C. Loss of Ptpn11 (Shp2) drives satellite cells into quiescence.. Elife 2017 May 2;6.
    pmc: PMC5441871pubmed: 28463680doi: 10.7554/elife.21552google scholar: lookup
  14. Gu JM, Wang DJ, Peterson JM, Shintaku J, Liyanarachchi S, Coppola V, Frakes AE, Kaspar BK, Cornelison DD, Guttridge DC. An NF-κB--EphrinA5-Dependent Communication between NG2(+) Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates.. Dev Cell 2016 Jan 25;36(2):215-24.
    doi: 10.1016/j.devcel.2015.12.018pmc: PMC4732710pubmed: 26777211google scholar: lookup
  15. Halevy O, Cantley LC. Differential regulation of the phosphoinositide 3-kinase and MAP kinase pathways by hepatocyte growth factor vs. insulin-like growth factor-I in myogenic cells.. Exp Cell Res 2004 Jul 1;297(1):224-34.
    doi: 10.1016/j.yexcr.2004.03.024pubmed: 15194438google scholar: lookup
  16. Jackson JR, Mula J, Kirby TJ, Fry CS, Lee JD, Ubele MF, Campbell KS, McCarthy JJ, Peterson CA, Dupont-Versteegden EE. Satellite cell depletion does not inhibit adult skeletal muscle regrowth following unloading-induced atrophy.. Am J Physiol Cell Physiol 2012 Oct 15;303(8):C854-61.
    doi: 10.1152/ajpcell.00207.2012pmc: PMC3469717pubmed: 22895262google scholar: lookup
  17. Kandalla PK, Goldspink G, Butler-Browne G, Mouly V. Mechano Growth Factor E peptide (MGF-E), derived from an isoform of IGF-1, activates human muscle progenitor cells and induces an increase in their fusion potential at different ages.. Mech Ageing Dev 2011 Apr;132(4):154-62.
    doi: 10.1016/j.mad.2011.02.007pubmed: 21354439google scholar: lookup
  18. Kawai M, Aida H, Hiraga A, Miyata H. Muscle satellite cells are activated after exercise to exhaustion in Thoroughbred horses.. Equine Vet J 2013 Jul;45(4):512-7.
    doi: 10.1111/evj.12010pubmed: 23206314google scholar: lookup
  19. Lee JD, Fry CS, Mula J, Kirby TJ, Jackson JR, Liu F, Yang L, Dupont-Versteegden EE, McCarthy JJ, Peterson CA. Aged Muscle Demonstrates Fiber-Type Adaptations in Response to Mechanical Overload, in the Absence of Myofiber Hypertrophy, Independent of Satellite Cell Abundance.. J Gerontol A Biol Sci Med Sci 2016 Apr;71(4):461-7.
    doi: 10.1093/gerona/glv033pmc: PMC5175449pubmed: 25878030google scholar: lookup
  20. Leshem Y, Gitelman I, Ponzetto C, Halevy O. Preferential binding of Grb2 or phosphatidylinositol 3-kinase to the met receptor has opposite effects on HGF-induced myoblast proliferation.. Exp Cell Res 2002 Apr 1;274(2):288-98.
    doi: 10.1006/excr.2002.5473pubmed: 11900489google scholar: lookup
  21. Leshem Y, Spicer DB, Gal-Levi R, Halevy O. Hepatocyte growth factor (HGF) inhibits skeletal muscle cell differentiation: a role for the bHLH protein twist and the cdk inhibitor p27.. J Cell Physiol 2000 Jul;184(1):101-9.
    doi: 10.1002/(SICI)1097-4652(200007)pubmed: 10825239google scholar: lookup
  22. Li J, Reed SA, Johnson SE. Hepatocyte growth factor (HGF) signals through SHP2 to regulate primary mouse myoblast proliferation.. Exp Cell Res 2009 Aug 1;315(13):2284-92.
    doi: 10.1016/j.yexcr.2009.04.011pmc: PMC2700832pubmed: 19393645google scholar: lookup
  23. von Maltzahn J, Jones AE, Parks RJ, Rudnicki MA. Pax7 is critical for the normal function of satellite cells in adult skeletal muscle.. Proc Natl Acad Sci U S A 2013 Oct 8;110(41):16474-9.
    doi: 10.1073/pnas.1307680110pmc: PMC3799311pubmed: 24065826google scholar: lookup
  24. MAURO A. Satellite cell of skeletal muscle fibers.. J Biophys Biochem Cytol 1961 Feb;9(2):493-5.
    pmc: PMC2225012pubmed: 13768451doi: 10.1083/jcb.9.2.493google scholar: lookup
  25. McCarthy JJ, Mula J, Miyazaki M, Erfani R, Garrison K, Farooqui AB, Srikuea R, Lawson BA, Grimes B, Keller C, Van Zant G, Campbell KS, Esser KA, Dupont-Versteegden EE, Peterson CA. Effective fiber hypertrophy in satellite cell-depleted skeletal muscle.. Development 2011 Sep;138(17):3657-66.
    doi: 10.1242/dev.068858pmc: PMC3152923pubmed: 21828094google scholar: lookup
  26. Miller KJ, Thaloor D, Matteson S, Pavlath GK. Hepatocyte growth factor affects satellite cell activation and differentiation in regenerating skeletal muscle.. Am J Physiol Cell Physiol 2000 Jan;278(1):C174-81.
    doi: 10.1152/ajpcell.2000.278.1.C174pubmed: 10644525google scholar: lookup
  27. Murach KA, Confides AL, Ho A, Jackson JR, Ghazala LS, Peterson CA, Dupont-Versteegden EE. Depletion of Pax7+ satellite cells does not affect diaphragm adaptations to running in young or aged mice.. J Physiol 2017 Oct 1;595(19):6299-6311.
    doi: 10.1113/JP274611pmc: PMC5621498pubmed: 28736900google scholar: lookup
  28. Murach KA, White SH, Wen Y, Ho A, Dupont-Versteegden EE, McCarthy JJ, Peterson CA. Differential requirement for satellite cells during overload-induced muscle hypertrophy in growing versus mature mice.. Skelet Muscle 2017 Jul 10;7(1):14.
    doi: 10.1186/s13395-017-0132-zpmc: PMC5504676pubmed: 28693603google scholar: lookup
  29. Müssig K, Staiger H, Fiedler H, Moeschel K, Beck A, Kellerer M, Häring HU. Shp2 is required for protein kinase C-dependent phosphorylation of serine 307 in insulin receptor substrate-1.. J Biol Chem 2005 Sep 23;280(38):32693-9.
    doi: 10.1074/jbc.M506549200pubmed: 16055440google scholar: lookup
  30. Paulsen G, Mikkelsen UR, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise?. Exerc Immunol Rev 2012;18:42-97.
    pubmed: 22876722
  31. Pawlikowski B, Vogler TO, Gadek K, Olwin BB. Regulation of skeletal muscle stem cells by fibroblast growth factors.. Dev Dyn 2017 May;246(5):359-367.
    doi: 10.1002/dvdy.24495pubmed: 28249356google scholar: lookup
  32. Perini I, Elia I, Lo Nigro A, Ronzoni F, Berardi E, Grosemans H, Fukada S, Sampaolesi M. Myogenic induction of adult and pluripotent stem cells using recombinant proteins.. Biochem Biophys Res Commun 2015 Aug 28;464(3):755-61.
    doi: 10.1016/j.bbrc.2015.07.022pubmed: 26164231google scholar: lookup
  33. Poli A, Mongiorgi S, Cocco L, Follo MY. Protein kinase C involvement in cell cycle modulation.. Biochem Soc Trans 2014 Oct;42(5):1471-6.
    doi: 10.1042/BST20140128pubmed: 25233434google scholar: lookup
  34. Quarta M, Brett JO, DiMarco R, De Morree A, Boutet SC, Chacon R, Gibbons MC, Garcia VA, Su J, Shrager JB, Heilshorn S, Rando TA. An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy.. Nat Biotechnol 2016 Jul;34(7):752-9.
    doi: 10.1038/nbt.3576pmc: PMC4942359pubmed: 27240197google scholar: lookup
  35. Reed SA, Ouellette SE, Liu X, Allen RE, Johnson SE. E2F5 and LEK1 translocation to the nucleus is an early event demarcating myoblast quiescence.. J Cell Biochem 2007 Aug 15;101(6):1394-408.
    doi: 10.1002/jcb.21256pubmed: 17295207google scholar: lookup
  36. Ronzoni F, Ceccarelli G, Perini I, Benedetti L, Galli D, Mulas F, Balli M, Magenes G, Bellazzi R, De Angelis GC, Sampaolesi M. Met-Activating Genetically Improved Chimeric Factor-1 Promotes Angiogenesis and Hypertrophy in Adult Myogenesis.. Curr Pharm Biotechnol 2017;18(4):309-317.
    doi: 10.2174/1389201018666170201124602pubmed: 28155605google scholar: lookup
  37. Sachs M, Brohmann H, Zechner D, Müller T, Hülsken J, Walther I, Schaeper U, Birchmeier C, Birchmeier W. Essential role of Gab1 for signaling by the c-Met receptor in vivo.. J Cell Biol 2000 Sep 18;150(6):1375-84.
    pmc: PMC2150711pubmed: 10995442doi: 10.1083/jcb.150.6.1375google scholar: lookup
  38. Sambasivan R, Yao R, Kissenpfennig A, Van Wittenberghe L, Paldi A, Gayraud-Morel B, Guenou H, Malissen B, Tajbakhsh S, Galy A. Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration.. Development 2011 Sep;138(17):3647-56.
    doi: 10.1242/dev.067587pubmed: 21828093google scholar: lookup
  39. Schaeper U, Vogel R, Chmielowiec J, Huelsken J, Rosario M, Birchmeier W. Distinct requirements for Gab1 in Met and EGF receptor signaling in vivo.. Proc Natl Acad Sci U S A 2007 Sep 25;104(39):15376-81.
    doi: 10.1073/pnas.0702555104pmc: PMC2000540pubmed: 17881575google scholar: lookup
  40. Schiaffino S, Dyar KA, Ciciliot S, Blaauw B, Sandri M. Mechanisms regulating skeletal muscle growth and atrophy.. FEBS J 2013 Sep;280(17):4294-314.
    doi: 10.1111/febs.12253pubmed: 23517348google scholar: lookup
  41. Schiaffino S, Mammucari C. Regulation of skeletal muscle growth by the IGF1-Akt/PKB pathway: insights from genetic models.. Skelet Muscle 2011 Jan 24;1(1):4.
    doi: 10.1186/2044-5040-1-4pmc: PMC3143906pubmed: 21798082google scholar: lookup
  42. Sheehan SM, Tatsumi R, Temm-Grove CJ, Allen RE. HGF is an autocrine growth factor for skeletal muscle satellite cells in vitro.. Muscle Nerve 2000 Feb;23(2):239-45.
    pubmed: 10639617doi: 10.1002/(sici)1097-4598(200002)23:2<239::aid-mus15>3.0.co;2-ugoogle scholar: lookup
  43. Stark DA, Karvas RM, Siegel AL, Cornelison DD. Eph/ephrin interactions modulate muscle satellite cell motility and patterning.. Development 2011 Dec;138(24):5279-89.
    doi: 10.1242/dev.068411pmc: PMC3222207pubmed: 22071104google scholar: lookup
  44. Strack V, Krützfeldt J, Kellerer M, Ullrich A, Lammers R, Häring HU. The Protein-tyrosine-phosphatase SHP2 is phosphorylated on serine residues 576 and 591 by protein kinase C isoforms alpha, beta 1, beta 2, and eta.. Biochemistry 2002 Jan 15;41(2):603-8.
    pubmed: 11781100doi: 10.1021/bi011327vgoogle scholar: lookup
  45. Tatsumi R, Hattori A, Ikeuchi Y, Anderson JE, Allen RE. Release of hepatocyte growth factor from mechanically stretched skeletal muscle satellite cells and role of pH and nitric oxide.. Mol Biol Cell 2002 Aug;13(8):2909-18.
    doi: 10.1091/mbc.E02-01-0062pmc: PMC117951pubmed: 12181355google scholar: lookup
  46. Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.. Am J Physiol Cell Physiol 2009 Apr;296(4):C922-9.
    doi: 10.1152/ajpcell.00471.2008pubmed: 19158401google scholar: lookup
  47. Walker N, Kahamba T, Woudberg N, Goetsch K, Niesler C. Dose-dependent modulation of myogenesis by HGF: implications for c-Met expression and downstream signalling pathways.. Growth Factors 2015;33(3):229-41.
    doi: 10.3109/08977194.2015.1058260pubmed: 26135603google scholar: lookup
  48. Webster MT, Fan CM. c-MET regulates myoblast motility and myocyte fusion during adult skeletal muscle regeneration.. PLoS One 2013;8(11):e81757.
    pmc: PMC3834319pubmed: 24260586doi: 10.1371/journal.pone.0081757google scholar: lookup
  49. Wozniak AC, Anderson JE. Nitric oxide-dependence of satellite stem cell activation and quiescence on normal skeletal muscle fibers.. Dev Dyn 2007 Jan;236(1):240-50.
    doi: 10.1002/dvdy.21012pubmed: 17117435google scholar: lookup
  50. Wozniak AC, Anderson JE. The dynamics of the nitric oxide release-transient from stretched muscle cells.. Int J Biochem Cell Biol 2009 Mar;41(3):625-31.
    doi: 10.1016/j.biocel.2008.07.005pubmed: 18694846google scholar: lookup
  51. Wozniak AC, Pilipowicz O, Yablonka-Reuveni Z, Greenway S, Craven S, Scott E, Anderson JE. C-Met expression and mechanical activation of satellite cells on cultured muscle fibers.. J Histochem Cytochem 2003 Nov;51(11):1437-45.
    doi: 10.1177/002215540305101104pmc: PMC3957553pubmed: 14566016google scholar: lookup
  52. Yablonka-Reuveni Z, Danoviz ME, Phelps M, Stuelsatz P. Myogenic-specific ablation of Fgfr1 impairs FGF2-mediated proliferation of satellite cells at the myofiber niche but does not abolish the capacity for muscle regeneration.. Front Aging Neurosci 2015;7:85.
    doi: 10.3389/fnagi.2015.00085pmc: PMC4446549pubmed: 26074812google scholar: lookup
  53. Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE. High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo.. Am J Physiol Cell Physiol 2010 Mar;298(3):C465-76.
    doi: 10.1152/ajpcell.00449.2009pmc: PMC2838568pubmed: 20007454google scholar: lookup

Citations

This article has been cited 5 times.
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  2. Barshick MR, Gonzalez ML, Busse NI, Helsel PJ, Johnson SE. The initial delay to mitotic activity in primary cultures of equine satellite cells is reduced by combinations of growth factors.. J Anim Sci 2022 Aug 1;100(8).
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  4. Gonzalez ML, Busse NI, Waits CM, Johnson SE. Satellite cells and their regulation in livestock.. J Anim Sci 2020 May 1;98(5).
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  5. Gonzalez ML, Jacobs RD, Ely KM, Johnson SE. Dietary tributyrin supplementation and submaximal exercise promote activation of equine satellite cells.. J Anim Sci 2019 Dec 17;97(12):4951-4956.
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