Identification and comparison of milk fat globule membrane and whey proteins from Selle Français, Welsh pony, and Tieling Draft horse mare’s milk.
Abstract: Horse's milk, with a high nutritional value and few allergenic proteins, could substitute cow's milk for infant consumption. Herein, a label-free, proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from three different horse breeds: Selle Français (SF), Welsh pony (WP), and Tieling Draft Horse (TDH). In MFGMs, 16 (SF), 66 (WP), and 45 (TDH) unique proteins were identified, which are involved in the endocytosis, ribosome, and staphylococcus aureus infection pathways, respectively. In whey, 31 (SF), 75 (WP), and 23 (TDH) unique proteins were identified, which are involved in the autophagy-animal, phenylalanine metabolism, and Vasopressin-regulated water reabsorption pathways, respectively. SF contained the lowest concentration of β-lactoglobulin, which can cause allergic reactions in humans. Our findings describe the nutritional differences and functional diversities of MFGM and whey proteins in different horse breeds, which could support developing formula more suitable for human infants.
Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Publication Date: 2023-11-02 PubMed ID: 37931453DOI: 10.1016/j.foodchem.2023.137915Google Scholar: Lookup
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Summary
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The research article discusses the protein profile of milk fat globule membrane (MFGM) and whey proteins in three breeds of horse milk: Selle Français, Welsh pony, and Tieling Draft Horse. The study found differences in their protein compositions and suggested that these mare milks may be used to develop infant formulas due to their lower concentrations of potentially allergenic components.
Objective and Methodology
- The goal of this research is to determine the nutritional differences and functional diversities of MFGM and whey proteins in three distinct horse breeds’ milk. These findings could potentially aid in developing more suitable human infant formulas.
- The researchers used a label-free, proteomic method to identify and compare the MFGM and whey proteins from milk samples collected from Selle Français (SF), Welsh pony (WP), and Tieling Draft Horse (TDH) breeds.
Findings
- In the MFGMs of each breed, the researchers identified unique proteins involved in various pathways. These proteins can be responsible for different physiological functions such as endocytosis, ribosome synthesis, and controlling bacterial infections.
- The whey proteins identified in the milk samples were involved in several pathways as well, which included the autophagy, phenylalanine metabolism, and Vasopressin-regulated water reabsorption pathways.
- Each breed’s milk had a different number of unique proteins identified, both in their MFGMs and whey fractions. This suggests significant diversity in protein composition across horse breeds. Selle Français breed milk in particular was found to have the lowest concentration of β-lactoglobulin, a protein known to cause allergic reactions in some individuals.
Implications
- The discovery of the vast diversity of proteins in horse milk, especially the lower concentration of potentially allergenic proteins such as β-lactoglobulin, suggests that horse milk may be a suitable substitute for cow’s milk in human infant formulas.
- Furthermore, the understanding of unique proteins in MFGM and whey proteins of different horse breeds contributes valuable information to their nutritional content and functions. This could guide the development of dairy products that are tailored to specific nutritional needs.
Cite This Article
APA
Lv K, Yang Y, Li Q, Chen R, Deng L, Zhang Y, Jiang N.
(2023).
Identification and comparison of milk fat globule membrane and whey proteins from Selle Français, Welsh pony, and Tieling Draft horse mare’s milk.
Food Chem, 437(Pt 2), 137915.
https://doi.org/10.1016/j.foodchem.2023.137915 Publication
Researcher Affiliations
- Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China; Research Unit of Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang, 110866, China.
- Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China; Research Unit of Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang, 110866, China.
- Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China; Research Unit of Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang, 110866, China.
- Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China; Research Unit of Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang, 110866, China.
- Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China.
- Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China; Research Unit of Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang, 110866, China. Electronic address: zhangyiwei@syau.edu.cn.
- Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China; Research Unit of Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang, 110866, China. Electronic address: jiangning@syau.edu.cn.
MeSH Terms
- Infant
- Cattle
- Horses
- Animals
- Female
- Humans
- Whey Proteins
- Proteomics / methods
- Milk Proteins
- Glycolipids
- Glycoproteins / genetics
- Lipid Droplets
- Allergens
Conflict of Interest Statement
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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