Immunohistochemical detection of P53 overexpression in paraffin wax-embedded squamous cell carcinomas of cattle, horses, cats and dogs.
Abstract: One hundred and six squamous cell carcinomas (SCCs) of cattle, horses, cats and dogs were analysed immunohistochemically for overexpression of p53 protein. The monoclonal antibody pAb 240, which recognizes only mutant p53, was used. Of 41 bovine ocular SCCs, 26 (63.4%) showed p53 nuclear reactivity. All of six (100%) equine ocular SCCs and seven of nine (77.7%) SCCs of the equine penis or vulva gave positive reactions. In nine of 11 (81.8%) feline SCCs of the ear and in seven of 14 (50%) feline SCCs of other locations, p53 immunoreactivity was detected. Only seven of 25 (29.5%) canine cutaneous SCCs gave a positive reaction. Thus p53 antigen could be detected immunohistochemically in formalin-fixed and paraffin wax-embedded tissues of SCCs of domestic animals. The results support the view that, as in man, p53 overexpression plays an important role in the development of most SCCs of the animal species studied. This was in particular true for feline SCCs of the ear and for bovine and equine ocular SCCs, which are assumed to be related to ultraviolet radiation.
Publication Date: 1996-02-01 PubMed ID: 8920221DOI: 10.1016/s0021-9975(96)80010-7Google Scholar: Lookup
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- Journal Article
Summary
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This study examined overexpression of the p53 protein in various types of squamous cell carcinomas (SCCs) in cattle, horses, cats, and dogs. The findings suggest that, similar to human cancers, p53 overexpression might play a significant role in the development of most SCCs in these animals, particularly in feline ear SCCs and bovine and equine ocular SCCs.
Research Methods
- The researchers conducted a study on a total of 106 squamous cell carcinomas (SCCs) found in diverse domestic animals including cattle, horses, cats, and dogs.
- The overexpression of p53 protein, which is a common indicator of many cancer types, was investigated in these SCCs using immunohistochemical analysis. This involves using antibodies to detect the presence and level of specific proteins in tissue samples.
- The specific antibody used in this case, pAb 240, reacts only with the mutant form of the p53 protein, indicating cells where the p53 gene has mutated and possibly leading to uncontrolled growth.
- Once the immunohistochemical analysis was performed, researchers tracked the p53 nuclear reactivity in these SCCs.
Research Findings
- Out of 41 bovine ocular SCCs, p53 overexpression was detected in about 63.4% of the cases.
- Overexpression of p53 was found in all six equine ocular SCCs examined and in 77.7% of equine penile or vulvar SCCs.
- Among feline SCCs, 81.8% of ear SCCs and 50% of SCCs from other locations showed p53 overexpression.
- Only 29.5% of the 25 canine cutaneous SCCs examined showed a positive reaction for p53 overexpression.
Conclusion and Implications
- Through these findings, the researchers concluded that overexpression of p53 seems to play a crucial role in the development of the majority of SCCs in domestic animals, similar to its role in human cancers.
- The overexpression was particularly prevalent in SCCs of the feline ear as well as bovine and equine ocular SCCs.
- The study gives rise to the hypothesis that these SCCs could be related to exposure to ultraviolet radiation, which is known to cause DNA damage potentially leading to mutations like those seen in the p53 gene.
Cite This Article
APA
Teifke JP, Löhr CV.
(1996).
Immunohistochemical detection of P53 overexpression in paraffin wax-embedded squamous cell carcinomas of cattle, horses, cats and dogs.
J Comp Pathol, 114(2), 205-210.
https://doi.org/10.1016/s0021-9975(96)80010-7 Publication
Researcher Affiliations
- Institut für Veterinär-Pathologie, Justus-Liebig-Universität, Giessen, Germany.
MeSH Terms
- Animals
- Carcinoma, Squamous Cell / chemistry
- Carcinoma, Squamous Cell / pathology
- Carcinoma, Squamous Cell / veterinary
- Cat Diseases / metabolism
- Cat Diseases / pathology
- Cats
- Cattle
- Cattle Diseases / metabolism
- Cattle Diseases / pathology
- Dog Diseases / metabolism
- Dog Diseases / pathology
- Dogs
- Female
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Male
- Paraffin Embedding
- Skin Neoplasms / chemistry
- Skin Neoplasms / pathology
- Skin Neoplasms / veterinary
- Tumor Suppressor Protein p53 / analysis
- Tumor Suppressor Protein p53 / biosynthesis
Citations
This article has been cited 12 times.- Sequeira I, Pires MDA, Leitão J, Henriques J, Viegas C, Requicha J. Feline Oral Squamous Cell Carcinoma: A Critical Review of Etiologic Factors.. Vet Sci 2022 Oct 11;9(10).
- Ludwig L, Dobromylskyj M, Wood GA, van der Weyden L. Feline Oncogenomics: What Do We Know about the Genetics of Cancer in Domestic Cats?. Vet Sci 2022 Oct 4;9(10).
- Gudenschwager-Basso EK, Stevenson V, Sponenberg DP, Cecere TE, Huckle WR. Characterization of the Expression of Angiogenic Factors in Cutaneous Squamous Cell Carcinoma of Domestic Cats.. Vet Sci 2022 Jul 21;9(7).
- Jasik A, Kycko A, Olech M, Wyrostek K, Śmiech A, Łopuszyński W, Otrocka-Domagała I, Mikiewicz M, Dolka I. Mutations of p53 Gene in Canine Sweat Gland Carcinomas Probably Associated with UV Radiation.. J Vet Res 2021 Dec;65(4):519-526.
- Renzi A, De Bonis P, Morandi L, Lenzi J, Tinto D, Rigillo A, Bettini G, Bellei E, Sabattini S. Prevalence of p53 dysregulations in feline oral squamous cell carcinoma and non-neoplastic oral mucosa.. PLoS One 2019;14(4):e0215621.
- Fornazari GA, Kravetz J, Kiupel M, Sledge D, Filho IRB, Montiani-Ferreira F. Ocular squamous cell carcinoma in Holstein cows from the South of Brazil.. Vet World 2017 Dec;10(12):1413-1420.
- Tmumen SK, Al-Azreg SA, Abushhiwa MH, Alkoly MA, Bennour EM, Al Attar SR. Cutaneous squamous cell carcinoma in the lateral abdominal wall of local Libyan ewes.. Open Vet J 2016;6(2):139-42.
- Couture ÉL, Langlois I, Santamaria-Bouvier A, Benoit-Biancamano MO. Cutaneous squamous cell carcinoma in an African pygmy hedgehog (Atelerix albiventris).. Can Vet J 2015 Dec;56(12):1275-8.
- Bocaneti F, Altamura G, Corteggio A, Velescu E, Borzacchiello G. Expression of bcl-2 and p53 in bovine cutaneous fibropapillomas.. Infect Agent Cancer 2015;10(1):2.
- Benazzi C, Al-Dissi A, Chau CH, Figg WD, Sarli G, de Oliveira JT, Gärtner F. Angiogenesis in spontaneous tumors and implications for comparative tumor biology.. ScientificWorldJournal 2014;2014:919570.
- Wypij JM. A naturally occurring feline model of head and neck squamous cell carcinoma.. Patholog Res Int 2013;2013:502197.
- Lanz OI, Wood BC. Surgery of the ear and pinna.. Vet Clin North Am Small Anim Pract 2004 Mar;34(2):567-99, viii.
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