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Home / Equine Research Bank / Vet Res Commun / Immunological and pathological investigations in equine expe...
Veterinary research communications2016; 40(3-4); 107-115; doi: 10.1007/s11259-016-9659-4

Immunological and pathological investigations in equine experimental uveitis.

Abstract: The pathogenic mechanism of equine recurrent uveitis (ERU) is still poorly defined and many variations between experimental animal models and spontaneous disease exist. Objective: The aim of our study was to investigate if Th17 cell-mediated response plays role in the pathogenesis of the used experimental model in horses and to reveal its pathological findings. Methods: Experimental uveitis was induced in 6 healthy horses. The concentrations of retinal autoantigen CRALBP and IL-17 were measured using ELISA in aqueous humor and vitreous body of the 12 inflamed eyes as well as in 12 control non-inflamed eyes taken from 6 horses in slaughter house. After centrifugation of the two eye media, smears were prepared and cytological investigation was performed. Tissue specimens were taken from all eye globes and were submitted to histopathological investigation. Results: CRALBP and IL-17 concentrations were significantly elevated in eye media of horses with experimental uveitis in comparison with controls. Cytological and histopathological findings corresponded to the changes characteristic of chronic immune-mediated inflammation with mononuclear cell infiltration of uvea, choroid, retina, and eye media as well as severe retinal destruction. Conclusions: Our study demonstrated the involvement of the retinal autoantigen CRALBP as well as IL-17 in the pathogenesis of experimental uveitis in horses. These findings suggests that this experimental uveitis in horses may serve as a suitable animal model for investigation of IL-17- mediated immune response during spontaneous autoimmune uveitis in horses as well as in humans.
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Publication Date: 2016-06-25 PubMed ID: 27344152DOI: 10.1007/s11259-016-9659-4Google Scholar: Lookup
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Summary

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The study discussed in the article investigates whether the Th17 cell-mediated response plays a role in the pathogenesis of experimental uveitis in horses, comparing concentrations of retinal autoantigen CRALBP and IL-17 in inflamed and non-inflamed eyes. The results indicate a significant involvement of retinal autoantigen CRALBP and IL-17 in the pathogenesis of experimental uveitis in horses.

Objectives of the Study

  • The primary aim of this research was to understand whether a Th17 cell-mediated response has a role in the pathogenesis of an experimental model of uveitis developed in horses.
  • The researchers set out to identify any pathological findings related to the disease.

Methods to Achieve Study Objectives

  • The researchers instigated Experimental uveitis in six healthy horses.
  • They measured the concentrations of retinal autoantigen CRALBP and IL-17 using an assay called ELISA (Enzyme-linked Immunosorbent Assay) in the aqueous humor and vitreous body of the inflamed eyes as well as in non-inflamed eyes taken from six other horses.
  • Cytological and histopathological investigations were carried out on tissue specimens from all affected eyes.

Key Results and Findings

  • The study found that concentrations of both CRALBP and IL-17 were significantly higher in the eyes of horses with experimental uveitis, when compared to control animals.
  • The researchers observed cytological and histopathological evidence of chronic immune-mediated inflammation with mononuclear cell infiltration of various ocular tissues, as well as severe retinal destruction.

Conclusions and Implications of the Study

  • The elevated concentrations of the retinal autoantigen CRALBP and IL-17 in the eye media of the horses with experimentally induced uveitis suggest their involvement in the disease’s pathogenesis.
  • These findings imply that the experimental uveitis in horses could potentially serve as a useful animal model for the investigation of the role of the IL-17-mediated immune response in spontaneous autoimmune uveitis in horses and humans.

Cite This Article

APA
Simeonova GP, Krastev SZ, Simeonov RS. (2016). Immunological and pathological investigations in equine experimental uveitis. Vet Res Commun, 40(3-4), 107-115. https://doi.org/10.1007/s11259-016-9659-4

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 40
Issue: 3-4
Pages: 107-115

Researcher Affiliations

Simeonova, Galina P
  • Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria. galinavet@abv.bg.
Krastev, Svetozar Z
  • Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.
Simeonov, Radostin S
  • Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria.

MeSH Terms

  • Animals
  • Aqueous Humor / chemistry
  • Aqueous Humor / immunology
  • Carrier Proteins / analysis
  • Carrier Proteins / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Eye / physiopathology
  • Horse Diseases / immunology
  • Horse Diseases / pathology
  • Horse Diseases / physiopathology
  • Horses
  • Interleukin-17 / analysis
  • Interleukin-17 / immunology
  • Th17 Cells / immunology
  • Uveitis / immunology
  • Uveitis / pathology
  • Uveitis / physiopathology
  • Uveitis / veterinary
  • Vitreous Body / chemistry
  • Vitreous Body / immunology
  • Vitreous Body / physiopathology

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Citations

This article has been cited 3 times.
  1. Dammak A, Pastrana C, Martin-Gil A, Carpena-Torres C, Peral Cerda A, Simovart M, Alarma P, Huete-Toral F, Carracedo G. Oxidative Stress in the Anterior Ocular Diseases: Diagnostic and Treatment.. Biomedicines 2023 Jan 20;11(2).
    doi: 10.3390/biomedicines11020292pubmed: 36830827google scholar: lookup
  2. Lu H, Chen DD, Zhu SQ. Therapeutic potential of pupilloplasty combined with phacomulsification and intraocular lens implantation against uveitis-induced cataract.. Int J Ophthalmol 2022;15(10):1598-1603.
    doi: 10.18240/ijo.2022.10.06pubmed: 36262864google scholar: lookup
  3. Degroote RL, Deeg CA. Immunological Insights in Equine Recurrent Uveitis.. Front Immunol 2020;11:609855.
    doi: 10.3389/fimmu.2020.609855pubmed: 33488614google scholar: lookup
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