In vitro cultivation of Plasmodium falciparum: studies with modified medium supplemented with ALBUMAX II and various animal sera.
Abstract: RPNI, a combination of three commercially available growth media (RPMI-1640, NCTC-135 and IMDM) has been found to support long term continuous cultivation of 3D7 strain of Plasmodium falciparum in the presence of 10% bovine calf serum. During the present study, the suitability of this medium was evaluated for the development of P. falciparum in the presence of horse, goat and rabbit sera as well as various concentrations of ALBUMAX II. RPNI medium supplemented with 10% bovine calf serum (RPNI-BCS) was used as control. The cultures were maintained in candle jars protocol and parasitaemia was monitored daily up to day 7. Horse, goat and rabbit sera all supported the development of P. falciparum. Horse serum gave best results in RPNI medium and supported continuous culture up to day 100. The parasitaemia in the presence of ALBUMAX was significantly higher in RPNI than in RPMI-1640. Addition of hypoxanthine in RPMI-1640 caused an increase in parasitaemia whereas no obvious advantage could be observed in RPNI. The findings exhibited that medium RPNI has an edge over conventional RPMI-1640 medium for in vitro cultivation of P. falciparum.
Publication Date: 2006-12-30 PubMed ID: 17275813DOI: 10.1016/j.exppara.2006.12.003Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates and demonstrates the efficiency of the RPNI medium, a combination of three commercial growth media, in the long-term cultivation of the Plasmodium falciparum parasite. The study finds that RPNI, supplemented with horse, goat, or rabbit serum and ALBUMAX II, outperforms conventional RPMI-1640 media in supporting the growth of P. falciparum.
Research Methods and Evaluation
- The team conducted their experiments with the 3D7 strain of Plasmodium falciparum, a parasite known for causing the harmful disease malaria.
- These parasites were grown in the presence of 10% bovine calf serum with RPNI as the primary medium. The efficacy of this method was then compared when horse, goat, and rabbit sera were used instead.
- Apart from this, the effect of various concentrations of ALBUMAX II, a nutritional supplement, on the parasites was also tracked.
- All these cultures were maintained in an environment following the candle jars protocol, where parasitaemia was checked each day until the seventh day.
Research Findings
- All three types of sera – horse, goat, and rabbit were found effective in growing P. falciparum.
- Of these, horse serum gave the best results in the RPNI medium and supported continuous culture up to 100 days.
- The cultures grown in the presence of ALBUMAX showed significantly higher parasitaemia in RPNI, as compared to in RPMI-1640.
- While the addition of hypoxanthine, a naturally occurring purine derivative, to RPMI-1640 resulted in an increase in parasitaemia, RPNI did not observe any perceptible advantages.
- The findings suggest that the RPNI medium may be more efficacious than the traditional RPMI-1640 medium for the in vitro cultivation of P. falciparum.
Cite This Article
APA
Srivastava K, Singh S, Singh P, Puri SK.
(2006).
In vitro cultivation of Plasmodium falciparum: studies with modified medium supplemented with ALBUMAX II and various animal sera.
Exp Parasitol, 116(2), 171-174.
https://doi.org/10.1016/j.exppara.2006.12.003 Publication
Researcher Affiliations
- Division of Parasitology, Central Drug Research Institute, Lucknow, India. kumkum1110@Yahoo.com
MeSH Terms
- Animals
- Cattle
- Culture Media / classification
- Goats
- Horses
- Hypoxanthine / physiology
- Plasmodium falciparum / growth & development
- Rabbits
- Serum / physiology
Citations
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