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The Journal of bone and joint surgery. British volume2002; 84(2); 276-288; doi: 10.1302/0301-620x.84b2.11167

Insulin-like growth factor-I enhances cell-based repair of articular cartilage.

Abstract: Composites of chondrocytes and polymerised fibrin were supplemented with insulin-like growth factor-I (IGF-I) during the arthroscopic repair of full-thickness cartilage defects in a model of extensive loss of cartilage in horses. Repairs facilitated with IGF-I and chondrocyte-fibrin composites, or control defects treated with chondrocyte-fibrin composites alone, were compared before death by the clinical appearance and repeated analysis of synovial fluid, and at termination eight months after surgery by tissue morphology, collagen typing, and biochemical assays. The structure of cartilage was evaluated histologically by Toluidine Blue reaction and collagen type-I and type-II in situ hybridisation and immunohistochemistry. Repair tissue was biochemically evaluated by DNA assay, proteoglycan quantitation and characterisation, assessment of collagen by reverse-phase high-performance liquid chromatography, and collagen typing using cyanogen bromide digestion and peptide separation by polyacrylamide gel electrophoresis. The results at eight months showed that the addition of IGF-I to chondrocyte grafts enhanced chondrogenesis in cartilage defects, including incorporation into surrounding cartilage. Gross filling of defects was improved, and the tissue contained a higher proportion of cells producing type-II collagen. Measurements of collagen type II showed improved levels in IGF-I-treated defects, supporting in situ hybridisation and immunohistochemical assessments of the defects. IGF-I improves the repair capabilities of chondrocyte-fibrin grafts in large full-thickness repair models.
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Publication Date: 2002-04-02 PubMed ID: 11922373DOI: 10.1302/0301-620x.84b2.11167Google Scholar: Lookup
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  • Non-U.S. Gov't
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study explores how adding Insulin-like growth factor-I (IGF-I) to chondrocyte grafts (cells promoting the growth of cartilage) enhances the repair of damaged cartilage in horses, leading to better integration of the repaired tissue into surrounding cartilage and a higher proportion of type II collagen-producing cells.

Explanation of the Experiment Methodology

  • The scientists used chondrocytes, cells that produce and maintain the extracellular matrix of cartilage, combined with polymerised fibrin, a protein involved in the clotting of blood.
  • This combination was further boosted with supplemental Insulin-like growth factor-I, a protein that has a vital role in childhood growth and continues to have anabolic effects in adults, to repair cartilage.
  • The experiment was conducted in a context of extensive loss of cartilage with the model comprising full-thickness cartilage defects in horses, which underwent arthroscopic repair.
  • The scientists compared the IGF-I and chondrocyte-fibrin composite group with control defects treated only with chondrocyte-fibrin composites, assessing results on clinical appearance, synovial fluid analysis before the animals were put down, and at the end of eight months via detailed tissue morphology, collagen typing, and biochemical assays.

Methods of Evaluation

  • Cartilage structure was evaluated via histological examination, specifically the Toluidine Blue reaction, and collagen type-I and type-II in situ hybridisation and immunohistochemistry.
  • The repaired tissue was evaluated biochemically via DNA assay, proteoglycan quantification, characterisation via high-performance liquid chromatography, and collagen typing methods using cyanogen bromide digestion and peptide separation.

Findings of the Study

  • The research at the end of eight months showed that adding IGF-I to chondrocyte grafts significantly boosts chondrogenesis (the process by which cartilage is formed) in cartilage defects. This includes better incorporation into surrounding cartilage.
  • The quality of defect filling was improved, and the tissue contained a higher proportion of type-II collagen-producing cells.
  • IGF-I-treated defects showed improved levels of type II collagen, supporting the in situ hybridisation and immunohistochemical assessments of the defects.
  • In conclusion, IGF-I enhances the repair capabilities of chondrocyte-fibrin grafts in full-thickness repair models.

Cite This Article

APA
Fortier LA, Mohammed HO, Lust G, Nixon AJ. (2002). Insulin-like growth factor-I enhances cell-based repair of articular cartilage. J Bone Joint Surg Br, 84(2), 276-288. https://doi.org/10.1302/0301-620x.84b2.11167

Publication

The Journal of bone and joint surgery. British volume
ISSN: 0301-620X
NlmUniqueID: 0375355
Country: England
Language: English
Volume: 84
Issue: 2
Pages: 276-288

Researcher Affiliations

Fortier, L A
  • Cornell University College of Veterinary Medicine, Ithaca, New York 14853, USA.
Mohammed, H O
    Lust, G
      Nixon, A J

        MeSH Terms

        • Animals
        • Cartilage, Articular / chemistry
        • Cartilage, Articular / drug effects
        • Cartilage, Articular / metabolism
        • Cartilage, Articular / pathology
        • Chondrogenesis / drug effects
        • Chondrogenesis / physiology
        • Collagen / analysis
        • Glycosaminoglycans / analysis
        • Horses
        • Immunohistochemistry
        • In Situ Hybridization
        • Insulin-Like Growth Factor I / pharmacology
        • Insulin-Like Growth Factor I / therapeutic use
        • Synovial Fluid / chemistry
        • Wound Healing / physiology

        Grant Funding

        • AR08360 / NIAMS NIH HHS

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