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Journal of inorganic biochemistry1980; 13(4); 305-316; doi: 10.1016/s0162-0134(00)80251-2

Iron mobilization from ferritin by chelating agents.

Abstract: The release of iron from horse spleen ferritin by the chelating agents desferrioxamine B, rhodotorulic acid, 2,3-dihydroxybenzoate, 2,2'-bipyridyl and pyridine-2-aldehyde-2-pyridyl hydrazone (Paphy) has been studied in vitro. Ferritin prepared by classical procedures involving thermal denaturation releases its iron less effectively than ferritin isolated by a modified procedure that avoids this step. Desferrioxamine B and rhodotorulic acid are the most effective in releasing iron from both preparations of ferritin. When FMN is added, iron release by desferrioxamine B, rhodotorulic acid, and 2,3-dihydroxybenzoate was effectively blocked, whereas both bipyridyl and Paphy showed a marked simulation. A substantial increase in iron release was also observed for bipyridyl and Paphy with ascorbate; a less important increase was noted for rhodotorulic acid. EDTA exerted a marked inhibition of iron release from ferritin with rhodotorulic acid, 2,3-dihydroxybenzoate, bipyridyl, and Paphy. The effects of citrate and oxalate on iron release by the chelators was small. The effect of the concentration of flavin on iron release from ferritin by bipyridyl and desferrioxamine B have been studied. Desferrioxamine is unable to mobilize FeII from ferritin following reduction by reduced FMN, whereas bipyridyl can rapidly complex the ferrous iron. The results are discussed in the context of our current concepts of storage iron mobilization in the treatment of iron overload.
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Publication Date: 1980-12-01 PubMed ID: 7193239DOI: 10.1016/s0162-0134(00)80251-2Google Scholar: Lookup
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  • Journal Article
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how different chelating agents can promote the release of iron from a protein called ferritin. The most efficient chelating agents were found to be desferrioxamine B and rhodotorulic acid, and other substances like FMN, ascorbate, and EDTA showed different levels of influence on the process.

Study Methodology

  • The researchers studied the ability of various chelating agents – desferrioxamine B, rhodotorulic acid, 2,3-dihydroxybenzoate, 2,2′-bipyridyl and pyridine-2-aldehyde-2-pyridyl hydrazone (Paphy) – to release iron from ferritin prepared from horse spleen. They tested this using two different preparation methods for ferritin to comprehend any difference linked with its preparation.

Findings on Chelating Agents

  • Desferrioxamine B and rhodotorulic acid were identified as the most effective agents to release iron from ferritin prepared in both ways. This implies these substances have a high binding efficiency with iron, allowing it to be mobilized from ferritin effectively.
  • The other three agents – 2,3-dihydroxybenzoate, 2,2′-bipyridyl, and Paphy – had varying effectiveness. Particularly, when FMN (flavin mononucleotide) was added, the iron release with these three agents showed different results. For instance, iron release by 2,3-dihydroxybenzoate was blocked, while Paphy and bipyridyl resulted in boosted iron release.

Influence of Other Substances

  • The inclusion of ascorbate significantly increased iron release in bipyridyl and Paphy. A smaller increase was seen with rhodotorulic acid.
  • EDTA (Ethylenediaminetetraacetic acid) was found to inhibit iron release from ferritin when paired with rhodotorulic acid, 2,3-dihydroxybenzoate, bipyridyl, and Paphy. This suggests that EDTA may interfere with these chelating agents’ ability to mobilize iron from ferritin.
  • The impacts of citrate and oxalate on iron release by the chelating agents were minimal, showing these substances don’t substantially impact the chelating abilities of examined agents.

Special Case of Flavin

  • The researchers further investigated the effect of flavin concentration on the iron release by specific chelating agents – bipyridyl and desferrioxamine B. Interestingly, while bipyridyl was able to mobilize FeII from ferritin quickly after reduction by reduced FMN, desferrioxamine could not.

The findings of this study provide valuable insights into how different chelating agents and substances can influence iron mobilization from ferritin. It is beneficial in the broader context of understanding the treatment of conditions like iron overload.

Cite This Article

APA
Crichton RR, Roman F, Roland F. (1980). Iron mobilization from ferritin by chelating agents. J Inorg Biochem, 13(4), 305-316. https://doi.org/10.1016/s0162-0134(00)80251-2

Publication

Journal of inorganic biochemistry
ISSN: 0162-0134
NlmUniqueID: 7905788
Country: United States
Language: English
Volume: 13
Issue: 4
Pages: 305-316

Researcher Affiliations

Crichton, R R
    Roman, F
      Roland, F

        MeSH Terms

        • Animals
        • Chelating Agents
        • Ferritins
        • Horses
        • Iron
        • Kinetics
        • Protein Binding
        • Spleen
        • Structure-Activity Relationship

        Citations

        This article has been cited 15 times.
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