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Methods in molecular biology (Clifton, N.J.)2025; 2938; 171-185; doi: 10.1007/978-1-0716-4607-6_19

Isolation and Characterization of Equine Mesenchymal Stromal/Stem Cells.

Abstract: Regenerative medicine is a relatively new branch of therapeutics in equine medicine, which aims to restore and reconstitute tissue function and structure via cellular and/or noncellular approaches. Biological constituents such as mesenchymal stromal/stem cells (MSCs) are potent therapeutics, which can aid in damaged tissue regeneration due to their differentiation capacity into many different cell types such as adipose tissue, bone, and cartilage. MSCs can be successfully and conveniently isolated from equine subcutaneous adipose tissue (adipose-derived stromal cells, ASCs). In horses, there are currently no standardized methods for characterizing ASCs both in research and clinical practice. However, many studies are investigating antibodies which may be expressed on the ASC cell surface through multicolor immunophenotyping. This chapter describes techniques used to isolate, grow, characterize, and store equine ASCs, which have been adapted from extensive published research from the Institute of Cellular and Molecular Medicine (ICMM) at the University of Pretoria.
© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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Publication Date: 2025-05-30 PubMed ID: 40445292PubMed Central: 8064371DOI: 10.1007/978-1-0716-4607-6_19Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the process of isolating, cultivating, characterizing, and storing mesenchymal stromal/stem cells (MSCs) from equine subcutaneous adipose tissue—techniques that could help advance regenerative medicine in horses.

Overview of Equine Regenerative Medicine

  • The study dives into the field of regenerative medicine, particularly in equine medicine. Regenerative medicine aims to restore and reconstitute tissue function and structure using cellular and noncellular approaches.
  • It introduces mesenchymal stromal/stem cells (MSCs) as an integral part of these therapeutics because of their ability to transform into a variety of cell types like adipose tissue, bone, and cartilage.
  • Consequently, MSCs could help in regenerating damaged tissue, making significant strides in the field of equine medicine.

Isolating Mesenchymal Stromal/Stem Cells

  • MSCs are isolated from subcutaneous adipose tissue in horses, resulting in what is known as adipose-derived stromal cells (ASCs). The process is highlighted as both convenient and successful.
  • However, there are currently no standard procedures for obtaining ASCs in research and clinical practice in horses.

Characterizing Mesenchymal Stromal/Stem Cells

  • Numerous ongoing studies are exploring specific antibodies that could be expressed on the ASC cell surface.
  • These antibodies could potentially play a key role in characterizing ASCs, and this is often carried out through multicolor immunophenotyping.

Techniques for Equine ASCs

  • The research outlines methods for isolating, growing, characterizing, and storing equine ASCs.
  • These methods have been adapted from extensive published research from the Institute of Cellular and Molecular Medicine (ICMM) at the University of Pretoria.
  • Understanding these techniques and their complexities would help researchers in applying regenerative medicine in equine practice more efficiently.

Cite This Article

APA
Bosman LM, Ambele MA, Pepper MS. (2025). Isolation and Characterization of Equine Mesenchymal Stromal/Stem Cells. Methods Mol Biol, 2938, 171-185. https://doi.org/10.1007/978-1-0716-4607-6_19

Publication

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
NlmUniqueID: 9214969
Country: United States
Language: English
Volume: 2938
Pages: 171-185

Researcher Affiliations

Bosman, Lisa Marie
  • Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Ambele, Melvin A
  • Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Pepper, Michael S
  • Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

MeSH Terms

  • Horses
  • Animals
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Cell Separation / methods
  • Cell Differentiation
  • Immunophenotyping / methods
  • Adipose Tissue / cytology
  • Cell Culture Techniques / methods
  • Flow Cytometry / methods
  • Cells, Cultured

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