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Home / Equine Research Bank / Vet Dermatol / Longitudinal pilot study examining the effect of punch biops...
Veterinary dermatology2024; doi: 10.1111/vde.13236

Longitudinal pilot study examining the effect of punch biopsy on equine sarcoid growth dynamics.

Abstract: Nonexcisional tissue biopsies facilitate pre-operative confirmation of equine sarcoid yet fear of lesion deterioration currently limits its use in the diagnostic workup. Objective: To evaluate the effect of a single punch biopsy on tumour growth dynamics [thickness, area, circumference, viral load (VL) and Visual Analog Scale (VAS)]. Methods: Six client-owned horses with 11 sarcoids of various classification. Methods: Growth dynamics were recorded on a weekly basis, 12 weeks pre- and 24 weeks post-biopsy. The effect of a single punch biopsy on growth dynamics was estimated by linear mixed-effect models. Spearman's rank correlation coefficient (SRCC) was calculated to investigate correlations with the number of weeks before and after the intervention. Results: While statistically significant post-biopsy changes were recorded for all parameters except VL, no parameter was consistently increased after the biopsy intervention. In two of 11 sarcoids, the VL correlation pattern revealed significant strong correlations: sarcoid 6 (pre: r = -0.66, p < 0.05; post: r = 0.81, p < 0.001), increased VL; and sarcoid 8 (pre: r = 0.85, p  0.05), no further increase after biopsy. Conclusions: Our results indicate that post-biopsy lesion deterioration is not a general concept that applies to all sarcoids, and both deterioration or improvement are possible outcomes over a 24-week period. Further clinical studies with a larger sample size are needed before a definitive conclusion can be made.
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Publication Date: 2024-01-02 PubMed ID: 38168049DOI: 10.1111/vde.13236Google Scholar: Lookup
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Summary

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The research investigated the impact of a single punch biopsy in the growth dynamics of equine sarcoid tumors, revealing that changes to the tumor are not consistently negative or positive post-biopsy.

Objective and Methodology

  • The main objective of the study was to understand the effect of a single punch biopsy on the growth dynamics of equine sarcoid tumors, specifically, changes in their thickness, area, circumference, viral load (VL), and Visual Analog Scale (VAS score).
  • This longitudinal pilot investigation was conducted on eleven sarcoids found in six client-owned horses with various sarcoid classifications.
  • To have a comprehensive view, the researchers monitored the growth dynamics of the tumors twelve weeks prior to and twenty-four weeks after the punch biopsy.
  • They utilized linear mixed-effect models to measure the change in growth dynamics caused by a single punch biopsy.
  • Additionally, Spearman’s rank correlation coefficient (SRCC) was calculated to determine correlations with the number of weeks before and after the biopsy procedure.

Results

  • The post-biopsy changes in thickness, area, circumference, VL and VAS were statistically significant for all parameters except VL. However, none of these parameters were found to consistently increase after the biopsy intervention.
  • In terms of the VL, two of the eleven sarcoids showed significant strong correlations. In sarcoid 6, the VL increased pre-biopsy (r = -0.66, p < 0.05) and greatly increased post-biopsy (r = 0.81, p < 0.001). Whereas in sarcoid 8, the VL significantly increased pre-biopsy (r = 0.85, p 0.05).

Conclusion

  • Contrary to the prevalent fear of sarcoid deterioration post-biopsy, the results suggest that lesion progression after biopsy is not a universal concept and can have different outcomes—either deterioration or improvement—over a 24-week period.
  • However, the researchers emphasize the need for further clinical studies with larger sample sizes to achieve a definitive conclusion on this matter.

Cite This Article

APA
Gysens L, Martens A, Haspeslagh M. (2024). Longitudinal pilot study examining the effect of punch biopsy on equine sarcoid growth dynamics. Vet Dermatol. https://doi.org/10.1111/vde.13236

Publication

Veterinary dermatology
ISSN: 1365-3164
NlmUniqueID: 9426187
Country: England
Language: English

Researcher Affiliations

Gysens, Lien
  • Department of Large Animal Surgery, Anaesthesia and Orthopaedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Martens, Ann
  • Department of Large Animal Surgery, Anaesthesia and Orthopaedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Haspeslagh, Maarten
  • Department of Large Animal Surgery, Anaesthesia and Orthopaedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Grant Funding

  • Bijzonder Onderzoeksfonds UGent

References

This article includes 15 references
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