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Home / Equine Research Bank / J Exp Med / Lymphocyte specificity to protein antigens. II. Fine specifi...
The Journal of experimental medicine1979; 149(2); 436-447; doi: 10.1084/jem.149.2.436

Lymphocyte specificity to protein antigens. II. Fine specificity of T-cell activation with cytochrome c and derived peptides as antigenic probes.

Abstract: Murine T-lymphocyte specificity was determined in a system of antigen driven in vitro T-cell proliferation using cytochrome c molecules from different species, their derived peptides and reconstituted hybrid proteins. It was observed that primed T cells could discriminate between peptide fragments which differed from each other at a single amino acid residue. These conclusions were substantiated by the pattern of cross-reactivity noted in the response of closely related cytochrome c proteins as well as when artificial hybrid molecules reconstituted by the covalent linkage of peptide fragments were analyzed. The pattern of specificity observed appeared to be haplotype (BDF1) dependent although similar conclusions about the fine specificity of T cells in the response to cytochrome c have been obtained in other strains but associated with different residues.
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Publication Date: 1979-02-01 PubMed ID: 84044PubMed Central: PMC2184807DOI: 10.1084/jem.149.2.436Google Scholar: Lookup
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  • Journal Article
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines how T-lymphocyte cells in mice respond to specific protein antigens, specifically cytochrome c or its fragment peptides. It discovered that the T cells could distinguish between peptides differing by only one amino acid.

Objective of the Study

  • The primary focus of this research is to understand the specifics of T-lymphocyte reactions to cytochrome c molecules and their derived peptides from distinct species. This understanding helps determine the precise immunogenicity level and the lymphocyte response to the perturbations in the antigen structure.

Methodology

  • The methodology involved the application of antigen-driven in vitro T-cell proliferation techniques. They used cytochrome c molecules, derived peptides, and reconstituted hybrid proteins from diverse species as antigenic probes. This allowed the detection of T-cell responses with high sensitivity.

Findings

  • The research identified that the T cells of primed mice could differentiate between peptide fragments differing by merely a single amino acid residue. This indicates a refined specificity in the immune response of these cells.
  • This finding was confirmed by the pattern of cross-reactivity detected in the responses to closely related cytochrome c proteins. It was also verified when artificial hybrid molecules, synthesized by covalently linking peptide fragments, were analyzed.

Implications

  • The detected pattern of T-cell specificity showed a haplotype (BDF1) dependency, suggesting a genetic influence on this fine-tuned immune response. However, similar findings about this specificity have been found in other strains, but associated with different residues. This leads to the conclusion that this refined level of T-cell immune response is a fundamental characteristic of lymphocytes, albeit potentially influenced by the host’s genetic context.

Cite This Article

APA
Corradin G, Chiller JM. (1979). Lymphocyte specificity to protein antigens. II. Fine specificity of T-cell activation with cytochrome c and derived peptides as antigenic probes. J Exp Med, 149(2), 436-447. https://doi.org/10.1084/jem.149.2.436

Publication

The Journal of experimental medicine
ISSN: 0022-1007
NlmUniqueID: 2985109R
Country: United States
Language: English
Volume: 149
Issue: 2
Pages: 436-447

Researcher Affiliations

Corradin, G
    Chiller, J M

      MeSH Terms

      • Amino Acid Sequence
      • Animals
      • Cattle
      • Cytochrome c Group / immunology
      • Epitopes
      • H-2 Antigens
      • Horses
      • Immunologic Memory
      • Lymph Nodes / immunology
      • Lymphocyte Activation
      • Mice
      • Peptide Fragments / immunology
      • Rabbits
      • Species Specificity
      • T-Lymphocytes / immunology

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