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Home / Equine Research Bank / J Mech Behav Biomed Mater / Mechanical degradation of TiO2 nanotubes with and without na...
Journal of the mechanical behavior of biomedical materials2016; 59; 508-518; doi: 10.1016/j.jmbbm.2016.02.028

Mechanical degradation of TiO2 nanotubes with and without nanoparticulate silver coating.

Abstract: The primary objective of this research was to evaluate the extent of mechanical degradation on TiO2 nanotubes on Ti with and without nano-particulate silver coating using two different lengths of TiO2 nanotubes-300nm and ~1µm, which were fabricated on commercially pure Titanium (cp-Ti) rods using anodization method using two different electrolytic mediums-(1) deionized (DI) water with 1% HF, and (2) ethylene glycol with 1% HF, 0.5wt% NH4F and 10% DI water. Nanotubes fabricated rods were implanted into equine cadaver bone to evaluate mechanical damage at the surface. Silver was electrochemically deposited on these nanotubes and using a release study, silver ion concentrations were measured before and after implantation, followed by surface characterization using a Field Emission Scanning Electron Microscope (FESEM). In vitro cell-material interaction study was performed using human fetal osteoblast cells (hFOB) to understand the effect of silver coating using an MTT assay for proliferation and to determine any cytotoxic effect on the cells and to study its biocompatibility. No significant damage due to implantation was observed for nanotubes up to ~1µm length under current experimental conditions. Cell-materials interaction showed no cytotoxic effects on the cells due to silver coating and anodization of samples.
Copyright © 2016 Elsevier Ltd. All rights reserved.
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Publication Date: 2016-03-03 PubMed ID: 27017285PubMed Central: PMC4860156DOI: 10.1016/j.jmbbm.2016.02.028Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates how much mechanical damage TiO2 nanotubes can endure when coated with nanoparticulate silver, also the biocompatibility of the silver-coated nanotubes, using different lengths and two testing methods.

Research Methodology

  • The researchers used two lengths of TiO2 nanotubes-300nm and around 1µm that were created on commercially pure Titanium (cp-Ti) rods through the anodization method.
  • The process of anodization was carried out in two different electrolytic solutions:
    • deionized (DI) water with 1% HF
    • ethylene glycol with 1% HF, 0.5% NH4F and 10% DI water.
  • These nanotubes fabricated rods were then inserted into equine cadaver bone to assess the extent of mechanical surface damage they could endure.

Silver Coating and Evaluation of Mechanical Degradation

  • The researchers proceeded to electrochemically deposit silver on these nanotubes.
  • The concentrations of silver ions were determined before and after implantation through a release study.
  • A Field Emission Scanning Electron Microscope (FESEM) was used for surface characterization afterwards.
  • Under the given experimental conditions, it was observed that for nanotubes up to around 1µm in length, significant damage because of implantation was absent.

In Vitro Study for Biocompatibility

  • An in vitro cell-material interaction study was carried out using human fetal osteoblast cells (hFOB) to understand the impact of silver coating.
  • This test also aimed at checking if there was a toxic effect on the cells due to the silver coating and anodization of samples, and assessing the materials’ biocompatibility.
  • The MTT assay was used for cell proliferation and determining any cytotoxic effect.
  • It was concluded that there were no cytotoxic effects on the cells due to the silver coating and the anodization of the samples, proving the biocompatibility of the silver coated nanotubes.

Cite This Article

APA
Shivaram A, Bose S, Bandyopadhyay A. (2016). Mechanical degradation of TiO2 nanotubes with and without nanoparticulate silver coating. J Mech Behav Biomed Mater, 59, 508-518. https://doi.org/10.1016/j.jmbbm.2016.02.028

Publication

Journal of the mechanical behavior of biomedical materials
ISSN: 1878-0180
NlmUniqueID: 101322406
Country: Netherlands
Language: English
Volume: 59
Pages: 508-518
PII: S1751-6161(16)30001-7

Researcher Affiliations

Shivaram, Anish
  • W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA.
Bose, Susmita
  • W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA.
Bandyopadhyay, Amit
  • W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA. Electronic address: amitband@wsu.edu.

MeSH Terms

  • Animals
  • Biocompatible Materials / chemistry
  • Cells, Cultured
  • Horses
  • Humans
  • Materials Testing
  • Metal Nanoparticles / chemistry
  • Nanotubes / chemistry
  • Osteoblasts / cytology
  • Silver / chemistry
  • Surface Properties
  • Titanium / chemistry

Grant Funding

  • R01 AR067306 / NIAMS NIH HHS

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Citations

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